QIAamp DNA Mini Kit
- Rapid purification of high-quality, ready-to-use DNA
- Consistent, high yields
- Complete removal of contaminants and inhibitors
The QIAamp DNA Mini Kit provides silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids, or washed cells from urine. No mechanical homogenization is necessary as the tissues are lysed enzymatically. Theconvenient spin-column procedure reduces hands-on preparation time to 20 minutes.Purification of DNA using the QIAamp DNA Mini Kit can be automated on theQIAcube Connect.
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Cat No./ID:51304 QIAamp DNA Mini Kit (50) $175.00 For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml) |
Cat No./ID:51306 QIAamp DNA Mini Kit (250) $757.00 For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml) |
Product Details
DNA purified using the QIAamp DNA Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, Southern blotting, SNP and STR genotyping, and pharmocogenomic research.
The QIAamp DNA Mini Kit yields DNA sized up to 50 kb (see figure "Purification of up to 50 kb genomic DNA"). DNA of this length denatures completely and has the highest amplification efficiency. Yields of nucleic acids or DNA depend on the starting material (see table).
QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.
| Sample | Amount | Total nucleicacid yields (µg)* | DNA yields(µg)† |
|---|---|---|---|
| Blood | 200 µl | 4–12 | 4–12 |
| Buffy coat | 200 µl | 25–50 | 25–50 |
| Cells | 106 | 20–30 | 15–20 |
| Liver | 25 mg | 60–115 | 10–30 |
| Brain | 25 mg | 35–60 | 15–30 |
| Lung | 25 mg | 25–45 | 5–10 |
| Heart | 25 mg | 15–40 | 5–10 |
| Kidney | 25 mg | 40–85 | 15–30 |
| Spleen | 10 mg | 25–45 | 5–30 |
Vacuum processing
Blood or other body fluids can be processed by vacuum, instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp Mini spin columns are accommodated on the QIAvac 24 Plus manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows these QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters.The QIAamp DNA Mini Kit is ideal for purification of DNA from most commonly used human tissue samples, including muscle, liver, heart, brain, bone marrow, and other tissues, swabs (buccal, eye, nasal, pharyngeal, and others), CSF, blood, body fluids, and washed cells from urine. DNA can be purified from up to 25 mg tissue or from up to 200µl fluid in 20 minutes, and eluted in 50–200 µl.
Specifications
Features | Specifications |
| Applications | PCR, Southern blotting |
| Elution volume | 50–200 µl |
| Format | Spin column |
| Main sample type | Whole blood, tissue, cells |
| Processing | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA, bacterial DNA, parasite DNA, viral DNA |
| Sample amount | 200 µl/25 mg/5 x 10e6 |
| Technology | Silica technology |
| Time per run or per prep | 20 minutes |
| Yield | 4–30 µg |
Product Resources
Product citations in scientific articles
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