Qiagen/QIAprep Spin Miniprep试剂盒/用于50个高纯度质粒minipreps:50个QIAprep 2.0自旋柱、试剂、缓冲液、收集管（2 ml）/27104
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¥1938.00
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1162.80
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3392242852
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4000-520-616
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商品介绍
QIAprep Spin Miniprep Kit
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For purification of up to 20 μg molecular biology grade plasmid DNA
- Ready-to-use plasmid DNA in minutes
- Reproducible yields of molecular biology grade plasmid DNA
- Single protocol for high- and low-copy vectors
- Even higher yields with the High-Yield Supplementary Protocol
- Improved QIAprep 2.0 Spin Column
- GelPilot loading dye for convenient sample analysis
The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. Even higher yields (up to 30 μg) can be achieved using the High-Yield Supplementary Protocol. The QIAprep Spin Miniprep Kit can be automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
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- Product Details
- Specifications
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Cat No./ID:27104 QIAprep Spin Miniprep Kit (50) $96.90 For 50 high-purity plasmid minipreps: 50 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml) |
Cat No./ID:27106 QIAprep Spin Miniprep Kit (250) $422.00 For 250 high-purity plasmid minipreps: 250 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml) |
Cat No./ID:27106X4 QIAprep Spin Miniprep Kit (1000) $1,360.00 For 1000 high-purity plasmid minipreps: 1000 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml) |
Cat No./ID:27115 QIAprep 2.0Spin Miniprep Columns (100 spin columns) $98.90 For high-purity plasmid minipreps |
The QIAprep Spin Miniprep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep 2.0 Spin Columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep 2.0 Spin Column handling options A, B, and C"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep 2.0 Spin Columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.
| Format | Spin columns |
| Purification module | QIAprep 2.0 Spin Columns |
| Throughput | 1–24 samples |
| Preparation time | 24 minipreps in 30 minutes |
| Equipment required | Microcentrifuge or vacuum manifold; fully automatable using the QIAcube |
| Lysate clearing | Centrifugation |
| Capacity of column reservoir | 800 µl |
| Minimum elution buffer volume | 50 µl |
| Culture volume for high-copy plasmids | 1–5 ml |
| Culture volume for low-copy plasmids/cosmids | 1–10 ml |
Purified DNA can be used in restriction digestion (see figure "Complete digestion with various restriction enzymes").
Principle
QIAprep 2.0 Spin Columnscontain a unique silica membrane that binds up to 20 μg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol–chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted fromQIAprep 2.0 Spin Columns is immediately ready to use– there is no need to precipitate, concentrate, or desalt.To enable faster and more convenient sample processing and analysis, gel loading dye is provided in the kit. GelPilot Loading Dye contains 3 tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").
Procedure
Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see flowchart "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep 2.0 module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water. In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.
Applications
The QIAprep Miniprep Kits provides reproducible yields of high-purity DNA suitable for use in most applications, including:
- PCR
- Restriction digestion
- Ligation and transformation
- Sequencing
- Screening
Specifications
Features | Specifications |
| Applications | Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation, etc. |
| Culture volume/starting material | 1–10 ml culture volume |
| Elution volume | 50 µl (minimal) |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing | Manual (vacuum or centrifugation) |
| Samples per run (throughput) | 1–24 samples per run |
| Technology | Silica technology |
| Time per run or prep per run | <30> |
| Yield | <20> |
Product Resources
Product citations in scientific articles
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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