QIAGEN Plasmid Plus Kits
For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA
- Rapid purification of up to 24 samples
- Fast vacuum-based parallel processing
- Low elution volumes for highly concentrated DNA
- Very low endotoxin levels (<1 EU/μg) due to dedicated wash buffer
The QIAGEN Plasmid PlusKits enable ultrafast, large-scale purification of up to
10mg of highly pure plasmid DNA. The use of a vacuum manifold allows purification of up to 24 samples in parallel, reducing hands-on time. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. The QIAGEN Plasmid Plus Kits also feature adedicated wash buffer for endotoxin reduction. The plasmid DNA obtained is highly suitable for a multitude of applications, including transfection into sensitive cell lines.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
Want to try this solution for the first time? Request a quote for a trial kit.
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Cat No./ID:12941 QIAGEN Plasmid Plus Midi Sample Kit (5) $68.80 QIAGEN Plasmid Plus Midi Sample Kit (5) |
Cat No./ID:12943 QIAGEN Plasmid Plus Midi Kit (25) $359.00 25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges |
Cat No./ID:12945 QIAGEN Plasmid Plus Midi Kit (100) $1,336.00 100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges |
Cat No./ID:12963 QIAGEN Plasmid Plus Maxi Kit (25) $729.00 25 QIAGEN Plasmid Plus Maxi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Maxi Cartridges |
Cat No./ID:12965 QIAGEN Plasmid Plus Maxi Kit (100) $2,726.00 100 QIAGEN Plasmid Plus Maxi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Maxi Cartridges |
Cat No./ID:12981 QIAGEN Plasmid Plus Mega Kit (5) $371.00 5 QIAGEN Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges |
Cat No./ID:12991 QIAGEN Plasmid Plus Giga Kit (5) $663.00 5 QIAGEN Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges |
Cat No./ID:19408 VacValves (24) $115.00 24 valves for use with the QIAvac 24 and QIAvac 24 Plus |
Cat No./ID:19418 QIAvac Holder $136.00 Holder for stabilizing QIAGEN Plasmid Plus Mega and Giga columns with Tube Extenders on the QIAvac 24 Plus |
Product Details
Consistent quality and performance
QIAGEN Plasmid Plus technology delivers the same performance and quality as anion-exchange technology. The unique binding chemistry of the QIAGEN Plasmid Plus columns ensures that pure plasmid DNA is obtained every time. High-yield protocols and extra buffer volumes are provided with the QIAGEN Plasmid Plus Kits. Yields of up to 10 mg (giga),2 mg (mega), 1 mg (maxi), and 250 μg (midi) of highly pure plasmid DNA, free of contaminants such as RNA, proteins, and genomic DNA are achieved (see figures "High yields are ensured with QIAGEN Plasmid Plus Kits (QPP)" and "Analytical chromatogram").
Transfection-grade plasmid DNA with very low endotoxin levels
Endotoxins, also known as lipopolysaccharides or LPS, are cell-membrane components of Gram-negative bacteria such as E. coli and are released during the lysis step of plasmid purification. Endotoxins can induce nonspecific activation of immune responses, and thus represent a noncontrollable variable in transfection experiment setup, influencing the outcome and reproducibility of results and making them difficult to compare and interpret.
Theendotoxin wash buffer provided in the kits reduces bacterial endotoxins to levels usually below 1 EU/μg DNA. (Endotoxin levels can vary depending on the host strain, culture media, and cell density. If the cell density is very high, endotoxin levels may be slightly higher, ranging from 1–3 EU/μg DNA.)This makes the plasmid DNA suitable for a multitude of applications, including transfection into sensitive cell lines (see figures "Endotoxin levels using QIAGEN Plasmid Plus Kits", "Endotoxin levels using different isolation methods", and"Efficient transfection in sensitive cell lines").
The QIAGEN Plasmid Plus Kits provide the most convenient method for large-scale plasmid preparation. The design and unique binding chemistry of the QIAGEN Plasmid Plus columns allow a simple bind-wash-elute procedure based on a novel chemistry. This procedure makes these kits the fastest large-scale prep available — 20 minutes for maxi and midipreps. The resulting plasmid DNA ishighly concentrated and ready for immediate use in subsequent applications without further ethanol precipitation. Thus, the plasmid DNAis highly suitable for applicationsthat only allow small reaction volumes.
Maximum plasmid yields using LyseBlue
LyseBlue, an optional color indicator that provides visual identification of optimum buffer mixing, is also included in the kit. This prevents common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, genomic DNA, and cell debris. Through a simple color change, this unique buffer additive ensures that optimum cell lysis and neutralization is achieved, maximizing yields.
| Features | Plasmid PlusGiga Kit | Plasmid Plus Mega Kit | Plasmid Plus Maxi Kit | Plasmid Plus Midi Kit |
| Applications | Transfection of most cell lines, cloning, enzymatic restriction,in-vitro translation/transcription, sequencing | Transfection of most cell lines, cloning, enzymatic restriction,in-vitro translation/transcription, sequencing | Transfection of most cell lines, cloning, enzymatic restriction,in-vitro translation/transcription, sequencing | Transfection of most cell lines, cloning, enzymatic restriction,in-vitro translation/transcription, sequencing |
| Culture volume/starting material | 2500 ml culture volume | 500 ml culture volume | 50–200 ml culture volume | 25–50 ml culture volume |
| Final DNA concentration | ≥1 µg/µl | ≥1 µg/µl | ≥1 µg/µl | ≥1 µg/µl |
| Plasmid type | Plasmids, cosmids | Plasmids, cosmids | Plasmids, cosmids | Plasmids, cosmids |
| Processing | Manual (centrifugation and vacuum), parallel processing | Manual (centrifugation and vacuum), parallel processing | Manual (centrifugation and vacuum), parallel processing | Manual (centrifugation and vacuum), parallel processing |
| Samples per run; throughput | 1–12 samples per run | 1–12 samples per run | 1–24 samples per run | 1–24 samples per run |
| Technology | Advanced silica technology (non-chaotrophic), endotoxin reduction | Advanced silica technology (non-chaotrophic), endotoxin reduction | Advanced silica technology (non-chaotrophic), endotoxin reduction | Advanced silica technology (non-chaotrophic), endotoxin reduction |
| Time per run | 50 min | 40 min | 20 min | 20 min |
| Yield | up to 10 mg | up to 2.5 mg | up to 1 mg | up to 250 µg |
The QIAGEN Plasmid Plus Kits provide transfection-grade plasmid DNA highly suited for all applications, such as:
- Transfection into most cell lines (e.g., sensitive cell lines such as Huh-7)
- Restriction digestion
- Cloning
- In vitro transcription and translation
- Sequencing
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