
QIAGEN Plasmid Kits
For purification of up to 10 mg transfection-grade plasmid or cosmid DNA
- Purity equivalent to 2 x CsCl gradient centrifugation
- High yields of plasmid DNA
- Cost-effective preparations
- LyseBlue for optimum lysis and maximum DNA yield
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:10023 QIAGEN-tip 20 (25) $225.00 25 columns |
Cat No./ID:10043 QIAGEN-tip 100 (25) $300.00 25 columns |
Cat No./ID:10063 QIAGEN-tip 500 (25) $638.00 25 columns |
Cat No./ID:10083 QIAGEN-tip 2500 (25) $1,319.00 25 columns |
Cat No./ID:10091 QIAGEN-tip 10000 (5) $545.00 5 columns |
Cat No./ID:12123 QIAGEN Plasmid Mini Kit (25) $227.00 25 QIAGEN-tip 20, Reagents, Buffers. |
Cat No./ID:12125 QIAGEN Plasmid Mini Kit (100) $732.00 100 QIAGEN-tip 20, Reagents, Buffers |
Cat No./ID:12143 QIAGEN Plasmid Midi Kit (25) $303.00 25 QIAGEN-tip 100, Reagents, Buffers |
Cat No./ID:12145 QIAGEN Plasmid Midi Kit (100) $1,133.00 100 QIAGEN-tip 100, Reagents, Buffers |
Cat No./ID:12145X4 QIAGEN Plasmid Midi Kit (400) $3,626.00 400 QIAGEN-tip 100, Reagents, Buffers |
Cat No./ID:12162 QIAGEN Plasmid Maxi Kit (10) $262.00 10 QIAGEN-tip 500, Reagents, Buffers |
Cat No./ID:12163 QIAGEN Plasmid Maxi Kit (25) $591.00 25 QIAGEN-tip 500, Reagents, Buffers |
Cat No./ID:12165 QIAGEN Plasmid Maxi Kit (100) $2,240.00 100 QIAGEN-tip 500, Reagents, Buffers |
Cat No./ID:12181 QIAGEN Plasmid Mega Kit (5) $325.00 5 QIAGEN-tip 2500, Reagents, Buffers |
Cat No./ID:12183 QIAGEN Plasmid Mega Kit (25) $1,328.00 25 QIAGEN-tip 2500, Reagents, Buffers |
Cat No./ID:12191 QIAGEN Plasmid Giga Kit (5) $568.00 5 QIAGEN-tip 10000, Reagents, Buffers |
Cat No./ID:19046 Plasmid Buffer Set $129.00 Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps |
Product Details
The QIAGEN Plasmid Kits uses gravity-flow QIAGEN anion-exchange tipsfor efficient purification of plasmid DNA. Up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), 100 µg (midi), and 20 µg (mini) high-copy plasmid DNA is purified from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium).Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications such as transfection (see figure "Transfection efficiency versus plasmid purification method"), cloning, and in vitro transcription.
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tipsoperate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.
Features | Plasmid Giga Kit | Plasmid Mega Kit | Plasmid Maxi Kit | Plasmid Midi Kit | Plasmid Mini Kit |
---|---|---|---|---|---|
Applications | Transfection, cloning, sequencing, capillary sequencing, etc. | Transfection, cloning, sequencing, capillary sequencing, etc. | Transfection, cloning, sequencing, capillary sequencing, etc. | Transfection, cloning, sequencing, capillary sequencing, etc. | Transfection, cloning, sequencing, capillary sequencing, etc. |
Culture volume/starting material | 2.5–5 litersculture volume | 500 ml – 2.5 liters culture volume | 100–500 ml culture volume | 25–100 ml culture volume | 3–10 ml culture volume |
Elution volume | Variable | Variable | Variable | Variable | Variable |
Plasmid type | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA |
Processing | Manual (centrifugation) | Manual (centrifugation) | Manual (centrifugation) | Manual (centrifugation) | Manual (centrifugation) |
Sample per run | 1 sample per run | 1 sample per run | 1 sample per run | 1 sample per run | 1 sample per run |
Technology | Anion-exchange technology | Anion-exchange technology | Anion-exchange technology | Anion-exchange technology | Anion-exchange technology |
Time per run | 320 min | 220min | 160 min | 150 min | 80 min |
Yield | <10 mg | <2.5 mg | <500 µg | up to 100 µg | <20 µg |
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and pure plasmid DNA is eluted in high-salt buffer (see flowchart "QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications, such as:
- Transfection
- Cloning
- PCR
- In vitro transcription
Specifications
Features | Specifications |
Applications | Transfection, cloning, sequencing, capillary sequencing etc. |
Culture volume/starting material | 2.5–5 liters culture volume |
Plasmid type | High-copy, low-copy, cosmid DNA |
Processing | Manual (centrifugation) |
Samples per run (throughput) | 1 sample per run |
Technology | Anion-exchange technology |
Time per run or prep per run | 320 min |
Yield | <10>10> |
Product Resources
Product citations in scientific articles
Customers who bought these products also bought
- Cat No./ID:19743
QIAfilter Midi Cartridges (25)
25 QIAfilter Midi Cartridges$232.00Add To Cart - Cat No./ID:19763
QIAfilter Maxi Cartridges (25)
25 QIAfilter Maxi Cartridges$360.00Add To Cart - Cat No./ID:19781
QIAfilter Mega-Giga Cartridges (5)
5 QIAfilter Mega-Giga Cartridges, Buffer FWB2$172.00Add To Cart - Cat No./ID:12943
QIAGEN Plasmid Plus Midi Kit (25)
25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges$359.00Add To Cart - Cat No./ID:12165X4
QIAGEN Plasmid Maxi Kit (400)
400 QIAGEN-tip 100, Reagents, BuffersAdd To Cart - Cat No./ID:12963
QIAGEN Plasmid Plus Maxi Kit (25)
25 QIAGEN Plasmid Plus Maxi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Maxi Cartridges$729.00Add To Cart - Cat No./ID:12981
QIAGEN Plasmid Plus Mega Kit (5)
5 QIAGEN Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges$371.00Add To Cart - Cat No./ID:12991
QIAGEN Plasmid Plus Giga Kit (5)
5 QIAGEN Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges$663.00Add To Cart - Cat No./ID:1043369
QIAamp DNA Blood Mini Accessory Set B
Additional Buffers and Reagents; Typically for use with 2x QIAamp DNA Blood Mini Kits (250), catalog number 51106, on the QIAcube$94.80Add To Cart - Cat No./ID:19048
EndoFree Plasmid Buffer Set
Buffers P1, P2, P3, QBT, QC, QN, ER, TE, Endotoxin-free H2O, RNase A; for 10 plasmid mega- or 5 gigapreps (endotoxin-free)$676.00Add To Cart - Cat No./ID:301425
Effectene Transfection Reagent (1 ml)
1 ml Effectene Reagent, Enhancer, Buffer; for 40 transfections in 60 mm dishes or 160 transfections in 12-well plates$357.00Add To Cart - Cat No./ID:335376
QIAseq FastSelect –rRNA/Globin Kit (24)
Includes3 tubes of QIAseq FastSelect reagent for rRNA removal and3 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for24 reactions from human, mouse and rat samples
$1,989.00Add To Cart - Cat No./ID:19055
Buffer QC (1000 ml)
1000 ml Wash Buffer$125.00Add To Cart - Cat No./ID:28704
QIAquick Gel Extraction Kit (50)
For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$124.00Add To Cart - Cat No./ID:28706X4
QIAquick Gel Extraction Kit (1000)
For gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$1,879.00Add To Cart - Cat No./ID:997008
Reagent Cartridge Holder (2)
Reagent cartridge holder for use with the QIAsymphony SP$47.00Add To Cart - Cat No./ID:19054
Buffer QBT (1000 ml)
1000 ml Equilibration Buffer$129.00Add To Cart - Cat No./ID:28104
QIAquick PCR Purification Kit (50)
For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$123.00Add To Cart - Cat No./ID:28106X4
QIAquick PCR Purification Kit (1000)
For purification of 1000 PCR reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)Add To Cart - Cat No./ID:12291
QIAfilter Plasmid Giga Kit (5)
5 QIAGEN-tip 10000, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges$634.00Add To Cart