QIAquick Gel Extraction Kit
For gel extraction/cleanup of up to 10 μg DNA (70 bp to 10 kb) from enzymatic reactions
- Up to 95% recovery of ready-to-use DNA
- Fast and convenient procedure
- Cleanup of DNA up to 10 kb in three easy steps
- Gel loading dye for convenient sample analysis
The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA rangingfrom 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on theQIAcube Connect.
For optimal results it is recommended to use this product together withQIAvac 24 Plus.
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Cat No./ID:28115 QIAquick Spin Columns (100) $125.00 For DNA fragment purification from PCR, gel fragments and enzymatic reactions |
Cat No./ID:28506 QIAquick PCR & Gel Cleanup Kit (100) $231.00 For purification PCR fragments and gel extractions of 100 reactions (at least 80–100 gel reactions or 100 PCRs) |
Cat No./ID:28704 QIAquick Gel Extraction Kit (50) $124.00 For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) |
Cat No./ID:28706 QIAquick Gel Extraction Kit (250) $579.00 For gel extraction or cleanup of 250 reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) |
Cat No./ID:28706X4 QIAquick Gel Extraction Kit (1000) $1,879.00 For gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) |
Product Details
QIAquick Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples (see figure "High recoveries from gels"). Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.
Gel loading dye
To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye containsthree tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").
Handling
QIAquick spin columns are designed to provide two convenient handling options. The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters. The QIAquick Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").
DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
Specifications
Features | Specifications |
| Binding capacity | 10 µg |
| Elution volume | 30–50 µl |
| Format | Tube |
| Fragment size | 70 bp – 10 kb |
| Processing | Manual |
| Recovery: oligonucleotides dsDNA | Recovery: dsDNA fragments |
| Removal <10mers 17–40mers="" dye="" terminator=""> | Removal <> |
| Sample type: applications | DNA: PCR reactions |
| Technology | Silica technology |
Product Resources
Product citations in scientific articles
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