QIAsymphony DNA Investigator Kit
- Purification from forensic and human identity samples
- More efficient yields from trace casework samples
- Higher signal-to-noise ratios
- Novel, prefilled reagent cartridges for ease of use
- High reproducibility through standardized processing
The QIAsymphony DNA Investigator Kit enables automated purification of genomic DNA from 1–96 samples, such as swabs, filters, casework or crime-scene samples, and blood on the QIAsymphony SP. Purification is fast and efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:931436 QIAsymphony DNA Investigator Kit (192) $1,133.00 For 192 preps of 200 µl each from casework and reference samples: includes 2 reagent cartridges and enzyme racks and accessories |
Product Details
The QIAsymphony DNA Investigator Kit enables highly efficient purification of DNA, even from very small amounts of sample material. The combination of sample input volumes of up to 1000 µl with elution volumes as low as 30 µl enables highly sensitive detection of DNA from a wide range of sample types, including diffuse stains.
The purification procedure is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute. The user can choose different elution volumes between 30 μl to 400 μl of water or modified TE Buffer (Buffer ATE), depending on the protocol.
Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions in the presence of proteinase K and Buffer ATL in total volumes of either 200 μl, 500 μl or 1000 μl.
There are two different kinds of DNA purification protocols, which can be used in conjunction with the pretreatment protocols.Casework protocols purify genomic and mitochondrial DNA from 200 μl, 500 μl or 1000 μl of lysate obtained from the sample lysis procedures detailed in the corresponding pretreatment protocols. DNA can be eluted in 30–200 μl of either water or modified TE Buffer (Buffer ATE). Reference protocols purify DNA from database samples, such as buccal swabs or dried blood. DNA can be eluted in 100–400 μl of Buffer ATE. The purified DNA is ready to use in downstream applications.
| Protocol | Lysis volume options | Enhanced binding | Eluate volume |
|---|---|---|---|
| Reference | 200, 500 μl | No | 100–400μl |
| Casework | 200, 500, 1000μl | No | 100–200 μl |
| Casework ADV | 200, 500, 1000 μl | Yes | 100–200 μl |
| Casework HE | 200, 500, 1000 μl | No | 30–80 μl |
| Casework HE ADV | 200, 500, 1000 μl | Yes | 30–80 μl |
The high-quality DNA obtained using the QIAsymphony DNA Investigator Kit is suitable for direct use in forensic, environmental, biodefense, biosecurity, and other applied-testing applications, such as:
- Genotyping, including fingerprinting and paternity analysis
- DNA purification from trace samples (e.g., crime-scene)
- DNA extraction from filters used in environmental testing
- Routine analysis of reference samples
Specifications
Features | Specifications |
| Applications | STR analysis, (real-time) PCR |
| Elution volume | Reference samples: 100-400 µl; Casework samples: 30- 200 µl |
| Input volume | Reference samples: 200 or 500 µl lysate; Casework samples: 200, 500 or 1000 µl lysate |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA |
| Sample type | Wide range of forensic and human-identity sample materials |
| Technology | Magnetic particle technology |
| Yield | < 3=""> |
Product Resources
Customers who bought these products also bought
- Cat No./ID:1055628
TopElute Fluid (60 ml)
60 mlTopElute Fluid for use withautomated nucleic acid purification protocols$26.60Add To Cart - Cat No./ID:381577
Investigator ESSplex SE QS Kit (400)
Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, Allelic Ladder ESSplex SE QS, DNA size standard 550 (BTO) and Nuclease-Free Water$7,934.00Add To Cart - Cat No./ID:997024
Filter-Tips, 1500 µl, Qsym SP (1024)
Disposable Filter-Tips, racked; (8 x 128). For use with the QIAsymphony SP/AS instruments$129.00Add To Cart - Cat No./ID:997002
Sample Prep Cartridges, 8-well (336)
8-well sample prep cartridges for use with the QIAsymphony SP$146.00Add To Cart - Cat No./ID:997004
8-Rod Covers (144)
8-Rod Covers for use with the QIAsymphony SP$103.00Add To Cart - Cat No./ID:19597
Investigator Lyse&Spin Basket Kit (50)
50 pouches containing 50 baskets and 100 collection tubes$47.00Add To Cart - Cat No./ID:387216
Investigator Quantiplex Pro Kit (200)
For use on Applied Biosystems 7500 Real-Time Systems: Quantiplex Pro Reaction Mix, Quantiplex Pro Primer Mix, Quantiplex Pro Control DNA M1, QuantiTect Nucleic Acid Dilution Buffer$901.00Add To Cart - Cat No./ID:13113-50
PowerBead Tubes, Ceramic 1.4 mm (50)
Ready to use bead tubes for rapid and reliable biological sample lysis from a wide variety of starting materials.$165.00Add To Cart - Cat No./ID:19598
Investigator Lyse&Spin Basket Kit (250)
10 pouches containing 5 x 50 baskets and 5 x 50 collection tubes$194.00Add To Cart - Cat No./ID:990332
Filter-Tips, 200 µl, (1024)
Disposable Filter-Tips, racked; (8 x 128). For use with the QIAcube and the QIAsymphony SP/AS instruments$104.00Add To Cart - Cat No./ID:386224
Matrix Standard BT6 (50)
Matrix standard for 6-FAM, BTG, BTY, BTR2, BTP, and BTO, for Applied Biosystems 3500 Genetic Analyzers$175.00Add To Cart - Cat No./ID:382417
Investigator 24plex QS Kit (400)
Primer mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder 24plex, DNA size standard 24plex (BTO), and nuclease-free water$9,239.00Add To Cart - Cat No./ID:387316
Investigator Quantiplex Pro RGQ Kit (200)
For use on QIAGEN RotorGene Q Real-Time Systems: Quantiplex Pro RGQ Reaction Mix, Quantiplex Pro RGQ Primer Mix, Male Control DNA M1, QuantiTect Nucleic Acid Dilution Buffer$934.00Add To Cart - Cat No./ID:952034
EZ1 DNA Investigator Kit (48)
For 48 preps: Reagent Cartridge (DNA Investigator), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer G2, Proteinase K, Carrier RNA$525.00Add To Cart - Cat No./ID:995908
Sample Prep Plates, 42-well, M48 (100)
Disposable polypropylene plates for sample preparation, including nucleic acid binding and washing steps; pack of 100$334.00Add To Cart - Cat No./ID:19588
Elution Microtubes CL (24 x 96)
Nonsterile polypropylene tubes (0.85 ml maximum capacity, less than 0.7 ml storage capacity, 0.4 ml elution capacity); 2304 in racks of 96; includes cap strips$624.00Add To Cart - Cat No./ID:990512
50 µl Conductive Filtered Tips
Carbon-impregnated conductive tips (960 tips) for use with liquid-level sensing; tips contain high-set filters; for use with Tip Rack Holder (cat. no. 9018949)$175.00Add To Cart - Cat No./ID:386041
Control DNA 9948 (5 ng/µl)
Control DNA for creation of positive PCR samples when preparing batches of Investigator 24plex QS and Investigator 24plex GO! STR PCR: 50 µl Control DNA$103.00Add To Cart - Cat No./ID:997102
Tubes, conical, 2 ml, Qsym AS (500)
Conical tubes (2 ml) for holding reagent; for use with the QIAsymphony AS$131.00Add To Cart - Cat No./ID:995652
Filter-Tips, 1000 µl, M48 (1000)
1000 Disposable Filter-Tips for use with the BioRobot M48$502.00Add To Cart
