DNeasy Plant Mini Kit
- Pure DNA, free from contaminants and enzyme inhibitors
- Rapid extraction of ready-to-use DNA
- No organic extraction, no ethanol precipitation
The DNeasy Plant Mini Kit provides fast and easy silica-based plant DNA extraction in convenient spin column format. Typical yields are 3–30 μg of high-quality DNA, depending on the samples used (e.g., wheat, maize, arabidopsis, tomato, tobacco). Extraction of plant DNA using the DNeasy Plant Mini Kit can be automated on the QIAcube Connect
DNeasy Plant Pro Kit – superior DNA yields and inhibitor removal from plant samples - See new kit.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:69104 DNeasy Plant Mini Kit (50) $243.00 50 DNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml) |
Cat No./ID:69106 DNeasy Plant Mini Kit (250) $1,083.00 250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml) |
Product Details
The DNeasy Plant Mini Kit allows rapid and efficient isolation of high-quality DNA from a wide variety of plant species and tissue types including the most demanding sources (see table "Selection of plant species processed with DNeasy Kits"). Samples may be fresh, frozen, or dried. The optimized DNeasy Plant procedure incorporates the QIAshredder spin column, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling following lysis.
| Abies alba (silver fir) | Nicotiana tabacum (tobacco) |
| Aesculus hippocastanum (horse chestnut) | Oryza sativa (rice)4 |
| Arabidopsis thaliana (thale cress) | Pelargonium sp. (geranium)4 |
| Avena sp. (oat) | Petunia sp.4 |
| Brassica napus (oilseed rape) | Pinus sylvestris (Scotch pine), P. brutia5 |
| Brassica oleracea (kohlrabi) | Populus tremula (aspen) |
| Chicorium endivia (chicory) | Pseudotsuga menziesii (Douglas fir) |
| Citrullini lanatus (water melon) | Quercus robur, Q. petrea (oak)6,7 |
| Egeria sp. | Rhododendron sp.2,4 |
| Fagus sylvatica (beech)1 | Rubus idaeus (raspberry) |
| Helianthus spp. (sunflower) | Solanum tuberosum (potato) |
| Hordeum vulgare (barley)2 | Sphagnum palustre (moss) |
| Humulus sp. (hops) | Spinacia oleracea (spinach) |
| Hydrilla sp. | Taxus baccata (yew) |
| Kalanchoe spp. | Triticum aestivum (wheat)4 |
| Lupinus sp. | Ulmus glabra (elm)6 |
| Lycopersicon esculentum (tomato)3 | Vitis spp. (grape)6 |
| Myriophyllum sp. | Zea mays (maize) |
The typical yield is 3–30 µg, with a sample size of up to 100 mg wet weight, and an elution volume of 50–400 µl. The DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample. Lower and higher range values correspond to arabidopsis and wheat, respectively.
The DNeasy Plant procedure yields pure nucleic acid, free of polysaccharides and other secondary metabolites often copurified using conventional methods. Such impurities can interfere with spectrophotometric readings and inhibit enzymatic reactions. Absorbance scans of DNeasy purified DNA show a symmetrical peak at 260 nm (see figures "DNA purity from oak leaves and pine needles"), confirming that the DNA is free of impurities, including enzyme inhibitors. DNeasy purified DNA is sized up to 40 kb (see figure "Pure DNA (20–25 kb) for restriction analysis"). Purified DNA can be used in a wide range of applications (see figures "PCR analysis" and "RAPD analysis").
DNeasy Plant Kits use advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. DNeasy technology replaces cumbersome DNA isolation procedures such as cetyltrimethylammonium bromide (CTAB), phenol, or chloroform extraction. Using the DNeasy procedure, alcohol precipitation is not necessary – purified DNA is ready for immediate use. DNeasy sample preparation technology is fully licensed.
Samples are first mechanically disrupted and then chemically lysed (see flowchart "DNeasy Plant and DNeasy 96 Plant procedures"). RNA is removed by RNAse digestion during lysis. Cell debries, precipitated proteins, and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted and the lysate is loaded onto the DNEasy Plant Mini spin column. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use.
The DNeasy Plant Mini Kit provides purification of DNA from plant tissue, including:
Specifications
Features | Specifications |
| Applications | PCR, real-time PCR, blotting |
| Elution volume | 50–400 µl |
| Format | Spin column |
| Main sample type | Plant samples |
| Processing | Manual |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | DNA |
| Sample amount | <100> |
| Technology | Silica technology |
| Time per run or per prep | <1> |
| Yield | 3–30 µg |
Product Resources
Product citations in scientific articles
Customers who bought these products also bought
- Cat No./ID:208252
QuantiNova Probe PCRKit (100)
For 100 x 20 µl reactions: 1 ml 2x QuantiNova Probe PCR Master Mix , 250 µl QN ROX Reference Dye, 500 µl QuantiNova Yellow Template Dilution Buffer, 1.9 ml Water$111.00Add To Cart - Cat No./ID:74904
RNeasy Plant Mini Kit (50)
50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers$402.00Add To Cart
