Qiagen/QIAamp 96 DNA QIAcube HT Kit/For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers/51331
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¥15820.00
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9492.00
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4000-520-616
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商品介绍
QIAamp 96 DNA QIAcube HT Kit
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For automated high-throughput isolation of total DNA from blood, cells,and tissues
- Simple and reliable automated processing for cost and time savings
- Standardized method suitable for a variety of sample types
- High yields even from specialized samples
- High-quality DNA that performs well in downstream analyses
- Optimized protocols for a range of starting materials
The QIAamp 96 DNA QIAcube HT Kit enables simple, automated purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from blood, cells,and tissue samples. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids that are ready for downstream analysis.This kit is designed to be used on QIAcube HTSystem.
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Cat No./ID:51331 QIAamp 96 DNA QIAcube HT Kit (5) $791.00 For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers |
Cat No./ID:950067 QIAcube HT Plasticware $321.00 Plasticware for 480 typical preps on QIAcube HT: 5S-Blocks, 5 Elution Microtubes RS (EMTR), 120x8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad |
The QIAamp 96 DNA QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The QIAamp 96 DNA QIAcube HT Kit provides fast and reliable automated purification of total DNA in 96-well format.Results are highly reproducible, with no significant well-to-well variation in DNA recovery (see figure Reproducible 96-well DNA purification). Automated purification of total DNA from blood samples is quick, easy, and reliable withthe QIAamp 96 DNA QIAcube HT Kit. Fresh or frozen whole blood containing common anticoagulants can be processed, and the resulting DNA is of the same high quality as from other QIAGEN purification solutions (see figures Agarose gel of purified genomic DNA and Analysis of genomic DNA from human blood).QIAcube HT and the dedicated QIAamp 96 DNA QIAcube HT Kit lets users increase sample purification throughput without having to compromise on quality or reliability.The procedureprovides high yields of pure DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions (see figures High performance in downstream assays andPurification of genomic DNA from tissues).
Principle
The QIAamp 96 DNAQIAcube HT Kit enables automated purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen tissues, cells, and blood. Samples treated with common anticoagulants such as citrate, heparin, or EDTA are suitable for use. The procedure yields high-quality DNA that performs well in PCR and other enzymatic reactions.The QIAamp 96 DNA QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
| Specification | Description |
|---|---|
| Number of samples | 24–96 samples (to be processed in increments of 8) |
| Sample input volume | Blood: up to 200 μl (for sample volumes less than 200 μl, add PBS)Cells: up to 5 x 106 cellsTissues: up to 20 mg tissue (10 mg for spleen, liver, or lung tissue) |
| Elutionvolume | 200 μl |
| Duration | 96 samples in approximately96 minutes 24 samples in approximately 45 minutes |
Procedure
The QIAamp 96 DNA QIAcube HT procedure is fast and simple with the QIAcube HT instrument. Samples are first lysed using QIAGEN Proteinase K. Buffering conditions are adjusted to provide optimal DNA binding conditions and the lysate is loaded onto the QIAamp 96 plate. As vacuum is applied, DNA selectively binds to the QIAamp membrane, and contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in three efficient wash steps. Pure DNA is eluted under vacuum in a single step in 200 µl of Buffer AE.
Applications
The QIAamp 96 DNA QIAcube HT Kit provides high-quality DNA, ready to use in various downstream assays, including applications in:Life science research Livestock breeding Pedigree genotyping Veterinary pathogen research Routine applied testing
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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