Qiagen/QIAamp 96 PowerFecal QIAcube HT试剂盒/50带有大珠的病原体裂解管，1瓶试剂DX/19092
市场价：
¥5240.00
美元价：
3144.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QIAamp 96 PowerFecal QIAcube HT Kit
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For automated high-throughput purification of genomic DNA from fresh or frozen stool samples that are high in PCR inhibitors
- Simple and reliable automated processing for cost and time savings
- No hazardous organic chemicals required
- Efficient removal of PCR inhibitors
- High sensitivity in downstream assays
The QIAamp 96 PowerFecal QIAcube HT Kit provides simple, automated purification of high-quality genomic DNA (human and bacterial) from up to 200 mg fresh or frozen stool samples using the QIAcube HT. The novel combination of MOBIO"s patented Inhibitor Removal Technology (IRT) and proven QIAamp purification system provides an optimized DNA purification solution for microbiome, infectious disease and metabolic research applications.
The QIAamp 96 PowerFecal QIAcube HT Kit is to be discontinued shortly. Try the DNeasy 96 PowerSoil Pro QIAcube HT Kit – for improved performance. Higher yields, better purity, more accurate representation of the microbial diversity.
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- Product Details
- Specifications
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Cat No./ID:19092 Pathogen Lysis Tubes L $262.00 50 Pathogen Lysis Tubes with large beads, 1 vial of Reagent DX |
Cat No./ID:19585 S-Blocks (24) $348.00 96-well blocks with 2.2 ml wells, 24 per case |
Cat No./ID:51531 QIAamp 96 PowerFecal QIAcube HT Kit (5) $1,734.00 For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers |
Cat No./ID:950067 QIAcube HT Plasticware $321.00 Plasticware for 480 typical preps on QIAcube HT: 5S-Blocks, 5 Elution Microtubes RS (EMTR), 120x8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad |
The QIAamp 96 PowerFecal QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
QIAcube HT and dedicated QIAcube HT purification kits let users increase sample purification throughput without having to compromise quality or reliability.
Effective removal of inhibitors
Stool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples.Principle
The QIAamp 96 PowerFecal QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
Procedure
The QIAamp 96 PowerFecal QIAcube HT procedure is fast and simple with the QIAcube HT instrument. PCR inhibitors are removed by the MOBIO proprietary Inhibitor Removal Technology (IRT) withtwo precipitation steps. A combination of mechanical disruption and proteinase K digestion ensure complete lysis of even difficult-to-lyse organisms, resulting in high concentrations ofunbiased DNA. Buffering conditions are adjusted to provide optimal DNA binding, and the lysates are loaded onto the QIAamp 96 plate. As vacuum is applied, DNA binds to the QIAamp membrane, while contaminants pass through. Remaining impurities are removed in four efficient wash steps. Pure DNA is eluted under vacuum in a single step in 100 µl of Buffer ATE.No hazardous organic chemicals are used, increasing laboratory and personal safety.
Applications
The high-quality nucleic acids are ready to be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease and metabolic research applications.
Specifications
Features | Specifications |
| Elution volume | 100 μl |
| Sample amount | Up to 200 mg fresh or frozen stool |
| Throughput | 24-96 samples in increments of 8 samples |
| Time per run | 96 samples in approximately 120 minutes 24 samples in approximately 60 minutes |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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