MinElute PCR Purification Kit
- Very small elution volumes
- Fast procedure and easy handling
- High, reproducible recoveries
- Gel loading dye for convenient sample analysis
The MinElute PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products 70 bp– 4 kb in size. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on theQIAcube Connect.
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Cat No./ID:28004 MinElute PCR Purification Kit (50) $141.00 50 MinElute Spin Columns, Buffers, Collection Tubes (2ml) |
Cat No./ID:28006 MinElute PCR Purification Kit (250) $633.00 250 MinElute Spin Columns, Buffers, Collection Tubes (2ml) |
Product Details
The MinElute PCR Purification Kit provides spin columns for PCR product cleanup. Using a microcentrifuge or vacuum manifold, high concentration ofDNAfragment (70 bp– 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick PCR Purification Kit.)
The MinElute PCR Purification Kit contains a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries.
Gel loading dye
To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").
The MinElute System uses a simple bind-wash-elute procedure. Binding buffer is added directly to the PCR sample or other enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Handling
MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus or QIAvac 6S with QIAvac Luer Adapters and can also be fully automated on the QIAcube (see figures "Spin column handling options A, B, C, D, and E").
DNA fragments purified with the MinElute System are ready for direct use in all applications, including:
- Sequencing, including next generation sequencing
- Microarray analysis
- Ligation and transformation
- Restriction digestion
- Labeling
Specifications
Features | Specifications |
| Binding capacity | 5 µg |
| Elution volume | 10 µl |
| Format | Tube |
| Fragment size | 70 bp – 4 kb |
| Processing | Manual |
| Recovery: oligonucleotides dsDNA | Recovery: oligonucleotides, dsDNA |
| Removal <10mers 17–40mers="" dye="" terminator=""> | Removal <> |
| Sample type: applications | DNA, oligonucleotides: PCR reactions |
| Technology | Silica technology |
Product Resources
Product citations in scientific articles
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