Qiagen/DNeasy PowerSoil Pro Kit/用于从所有土壤类型中分离微生物基因组DNA。/47014
市场价：
¥7140.00
美元价：
4284.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
DNeasy PowerSoil Pro Kit
Print
For the isolation of microbial genomic DNA from all soil types
- Efficient lysis of bacteria and fungi in all soil types
- Up to 8-fold higher DNAyields compared to alternative methods
- Recovery of inhibitor-free DNAfor direct usein NGS applications
- Unbiased resultsaccurately representsample alphadiversity
- Automation on the QIAcube
Extracting microbial DNA from soil samples can be challenging. QIAGEN’s new DNeasy PowerSoil Pro Kit is even more effective than our original PowerSoil technology at isolating high yields of pure microbial DNA from all soil types, including compost, clay and top soil. The kit features a novel bead tube and optimized chemistry for more efficient lysis of soil bacteria and fungi. The kit also contains streamlined Inhibitor Removal Technology (IRT) to eliminate the challenging inhibitors commonly found in soil and environmental samples in even less time. Sequencing results reveal higher alpha diversity as measured by observed operational taxonomic units (OTUs) compared to other tested methods. Extraction of DNA using the DNeasy PowerSoil Pro Kit can be automated on theQIAcube Connect.Want to try this solution for the first time? Request a quote for a trial kit.
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- Product Details
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Cat No./ID:47014 DNeasy PowerSoil Pro Kit (50)Discover QIAGEN CLC Genomics ProSuite $357.00 For the isolation of microbial genomic DNA from all soil types. |
Cat No./ID:47016 DNeasy PowerSoil Pro Kit (250)Discover QIAGEN CLC Genomics ProSuite $1,614.00 For the isolation of microbial genomic DNA from all soil types. |
The DNeasy PowerSoil Pro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Significantly increased DNA yields from challenging samples
The DNeasy PowerSoil Pro Kitincludes a novel bead tube and improved lysis chemistry, which enables isolation of up to 8-fold higher yields of DNA compared to the first generation DNeasy PowerSoil Kit and those of competitors in all soil types tested (See: "Isolate more high-quality genomic DNA."). Both the DNeasy PowerSoil Kit and the new DNeasy PowerSoil Pro Kit isolated high-quality DNA from challenging soil types (See: "Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit.").Unbiased identification of total diversity and community representation in soil samples
DNA isolated using theDNeasy PowerSoil Pro Kitidentified more bacteria (OTUs) in soil samples when compared with the DNeasy PowerSoil Kit and those oftwo competitors (See: "Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit.").Principle
Inhibitor Removal Technology increases purity of isolated DNA
The DNeasy PowerSoil Pro Kitfeatures a streamlined Inhibitor Removal Technology (IRT) to decrease sample processing time. In a comparison of methods for DNA isolation from soil, the DNeasy PowerSoil Pro Kit was the only method showing 260/280 ratios near 1.8 for all soil types, as well as the highest 260/230 ratios, indicating the absence of inhibitors. Samples isolated using the kit also showed no inhibition in PCR, in contrast to competitor methods (See: "Increased purity and less variability in DNA extracted with the DNeasy PowerSoilPro Kit.").Procedure
The soil or stool samples are lysed via chemical and mechanical homogenization. Lysis buffer is added to a mixed zirconium bead tube containing the sample. Bead beating can be carried out using a standard benchtop vortex with bead tube adapter or the high-powered PowerLyser. Crude lysate is then subjected to inhibitor removal for cleanup. Following the cleanup, purified lysate is mixed with an equal volume of DNA binding solution and passed through a silica spin filter membrane. The membrane is washed with a two-step washing regime. Silica-bound DNA is then eluted using a 10 mM Tris elution buffer. Relevant downstream applications for the isolated DNA include PCR, NGS and enzymatic digestion assays.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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