Qiagen/DyeEx 2.0 Spin Kit/50 DyeEx Spin Columns, Collection Tubes (2 ml)/63204
市场价：
¥3880.00
美元价：
2328.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
DyeEx 2.0 Spin Kit
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For removal of unincorporated dye terminators from 1–24 sequencing reactions
- Fast procedure with only two short centrifugation steps
- Ready-to-use prehydrated gel-filtration material
- Efficient removal of any dye terminator
DyeEx Kits provide either spin columns or 96-well plates and use gel-filtration technology to remove dye terminators from sequencing reactions. Sequencing reactions are loaded onto the pre-hydrated gel-filtration material. After a short centrifugation step, the reactions are ready to be loaded onto a capillary sequencer. Unincorporated dye terminators are retained in the gel matrix.
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Cat No./ID:63204 DyeEx 2.0 Spin Kit (50) $194.00 50 DyeEx Spin Columns, Collection Tubes (2 ml) |
Cat No./ID:63206 DyeEx 2.0 Spin Kit (250) $875.00 250 DyeEx Spin Columns, Collection Tubes (2 ml) |
The DyeEx 2.0 Spin Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
DyeEx Kits remove any type of dye terminator from 10–20 µl sequencing reactions including BigDye, dRhodamine dye, Rhodamine, DYEnamic ET, and WellRED terminators. After cleanup, the sequencing reactions can be separated on any capillary sequencer. We recommend the DyeEx 96 Kit using the optimized protocol.
High-quality sequencing
DyeEx Kits are optimized for fast and convenient dye-terminator removal leading to high-quality sequencing results. In contrast, sequencing-reaction cleanup by ethanol precipitation is very time consuming (see table "Comparison of DyeEx procedure and ethanol precipitation") and inefficient. The failure to remove dye terminators efficiently often leads to the appearance of dye blobs in sequencing data making stretches of sequence unreadable. Using DyeEx Kits for sequencing reaction cleanup ensures that the reactions loaded onto sequencing instruments are free of dye terminators. Without dye blobs, the sequence is easily readable throughout (see figure "Superior sequencing results"). Signal intensities are high, resulting in long read lengths (see figure "Long-read sequence").
| Procedure | DyeEx 2.0 Spin | Ethanolprecipitation |
|---|---|---|
| Time required*(12 samples, spin format) | 10 minutes | >45 minutes |
| Time required*†(Microplate format for 96 samples) | N.A. | >60 minutes |
| Handling | Ready-to-usespin format | Multiplepipetting steps |
| Sequence quality | ++ | + |
Principle
The DyeEx dye-terminator removal system is designed for fast and efficient removal of unincorporated dye terminators from sequencing reactions using a simple spin column procedure. The DyeEx procedure uses gel filtration to quickly and efficiently remove unincorporated terminators from sequencing reactions. Removal of dye terminators is important to prevent the unincorporated dye terminators from interfering with analysis of sequencing results. The DyeEx gel-filtration material consists of spheres with uniform pores and separates molecules according to molecular weight. When sequencing reaction mixtures are applied to DyeEx columns, dye terminators diffuse into the pores and are retained in the gel-filtration material, while labeled DNA fragments are excluded and recovered in the flow-through (see figure "DyeEx separation principle").
Procedure
Dye-terminator removal with DyeEx Kits is fast because the procedure is so simple (see flowchart "DyeEx 2.0 Spin procedure"). A quick centrifugation step removes storage buffer from the columns, the sequencing samples are loaded, and a second centrifugation step removes unincorporated dye terminators. Samples are then ready for direct loading onto a capillary sequencer or can be dried, redissolved, and loaded onto a sequencing gel.
Applications
After cleanup, the sequencing reactions can be separated on any capillary sequencer.
Specifications
Features | Specifications |
| Binding capacity | 10–20 µl |
| Elution volume | 10–20 µl |
| For DyeEx Kits: | Terminator removal, ABI PRISM 377, 373, 310, 3100, or 3700, MegaBACE 1000, or CEQ 2000 sequencers |
| Format | Tube/96-well plate |
| Processing | Manual |
| Removal <10mers 17–40mers="" dye="" terminator=""> | Dye terminator |
| Sample type: applications | Sequencing reactions |
| Technology | Gel filtration |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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