Qiagen/QIAamp DNA Micro Kit/For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)/56304
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¥5020.00
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3012.00
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3392242852
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4000-520-616
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商品介绍
QIAamp DNA Micro Kit
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For purification of genomic and mitochondrial DNA from small samples
- Rapid purification of high-quality DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
The QIAamp DNA Micro Kit simplifies purification of genomic and mitochondrial DNA with a fast spin-column procedure. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl. Purification of DNA using the QIAamp DNA Micro Kit can be automated on the QIAcube.
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Cat No./ID:56304 QIAamp DNA Micro Kit (50) $251.00 For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml) |
The QIAamp DNA Micro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The resulting purified DNA is free of proteins, nucleases, and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure "Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure "Laser microdissection PCR"). DNA purified with the QIAamp DNA Micro Kit may also be used in short-tandem repeat (STR) genotyping, single-nucleotide pylomorphism (SNP) genotyping, or pharmacogenomic research.
Principle
The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µl. DNA binds specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA Micro technology yields genomic and mitochondrial DNA from small samples ready to use in PCR and blotting procedures.
QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.
Procedure
The QIAamp DNA Micro Kit simplifies isolation of DNA from small samples with a fast spin-column procedure (see flowchart "Procedure"). The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1–100 µl whole blood, blood cards of ~3 mm diameter, urine, or <10 mg tissue or laser microdissected tissue). Fresh or frozen blood, tissue, and dried blood spots can be purified in 30 minutes and eluted in 20–100 µl.
Applications
The QIAamp DNA Micro Kit procedure is suitable for a wide range of sample materials, including:
- Small volumes of blood
- Blood cards
- Urine
- Small tissue samples, including laster microdissections
Specifications
Features | Specifications |
| Applications | Real-time PCR, STR analysis, LMD-PCR |
| Elution volume | 20–100 µl |
| Format | Spin column |
| Main sample type | Whole blood |
| Processing | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA |
| Sample amount | 1–100 µl |
| Technology | Silica technology |
| Time per run or per prep | 30 minutes |
| Yield | <3> |
Product Resources
Product citations in scientific articles
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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