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当前位置: 首页 > 产品中心 > peptide > Qiagen/QIAamp PowerFecal Pro DNA试剂盒/用于从粪便和肠道样本中分离微生物DNA
商品详细Qiagen/QIAamp PowerFecal Pro DNA试剂盒/用于从粪便和肠道样本中分离微生物DNA
Qiagen/QIAamp PowerFecal Pro DNA试剂盒/用于从粪便和肠道样本中分离微生物DNA
Qiagen/QIAamp PowerFecal Pro DNA试剂盒/用于从粪便和肠道样本中分离微生物DNA
商品编号: 51800
品牌: Qiagen
市场价: ¥7120.00
美元价: 4272.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

QIAamp PowerFecal Pro DNA Kit

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For the isolation of microbial DNA from stool and gut samples
  • Efficient lysis of bacteria and fungi from stool and gut samples
  • Up to 20-fold more DNA compared to alternative methods
  • Yield higher alpha diversity in sequencing compared to other methods
  • Recovery of inhibitor-free DNA for direct use in next-generation sequencing applications
  • Automate workflows on the QIAcube Connect

Extracting microbial DNA from stool and gut samples can be challenging. Researchers will isolate higher yields of pure microbial DNA from stool and gut samples more efficiently with our new QIAamp PowerFecal Pro DNA Kit.

The QIAamp Power Fecal Pro DNA Kit builds and improves on our original PowerFecal technology utilizing a novel bead tube and optimized chemistry for more efficient lysis of bacteria and fungi. Inhibitor removal has been streamlined, allowing researchers to eliminate challenging inhibitors from stool and gut samples faster. This results in total DNA that reveals higher alpha diversity through observed operational taxonomic units (OTUs) in downstream testing.

Isolation of DNA using the QIAamp PowerFecal Pro DNA Kit can be automated on the QIAcube Connect.

Want to try this solution for the first time? Request a quote for a trial kit.

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Cat No./ID:51800
QIAamp PowerFecal Pro DNA Sample (2)
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For the isolation of microbial DNA from stool and gut samples
Cat No./ID:51804
QIAamp PowerFecal ProDNA KitDiscover QIAGEN CLC Genomics ProSuite
$356.00
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For the isolation of microbial DNA from stool and gut samples
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The QIAamp PowerFecal Pro DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

9
The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high-quality DNA.
A DNAfrom duplicate 1.8 ml C. albicans cultures was isolated with the QIAamp PowerFecal DNA (A) or the QIAamp PowerFecalPro DNA (B) Kits. B, C DNA from stool samples (200 mg) from three donors was prepared with the first-generation QIAampPowerFecal DNA Kit B or the new QIAamp PowerFecal Pro DNA Kit C. DNA was analyzed via gel electrophoresis.
9
Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.

Stool samples (200 mg) were prepared using commercially available sample preparation solutions or the new QIAamp PowerFecal Pro DNA Kit and compared. DNA purity was measured via UV spectrophotometry and the comparisons of A260/A280 A and A260/A230 B ratios are shown.

9
Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.
DNAprepared from stool samples was isolated with differentmethods and enriched. Analysis of the 16S rRNA genes was done using the QIAseq® 1-Step Amplicon Kit and dataanalysis was done using the Microbial Genomics Pro DNASuite (CLC workbench). Alpha diversity was determined bytotal number of bacterial OTUs.
9
Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.
DNA was isolated from stool samples (200 mg) from three donors using commercially available sample preparation solutions and the new QIAamp PowerFecal Pro DNA Kit and compared. Yields were measured by fluorometric quantification (Qubit™).
Performance

Using improved technologies, the new QIAamp PowerFecal Pro Kit allows researchers to obtain higher yields of DNA from stool and gut samples compared with alternative methods (See: "Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.").Improved yields of higher quality DNA are also achieved when comparing the QIAamp PowerFecal Pro DNA Kit to the first generation QIAamp PowerFecal DNA Kit when running stool and fungal samples (See: "The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high quality DNA.").

Principle

Thestreamlined Inhibitor Removal Technology® (IRT)used by the new QIAamp PowerFecal Pro DNA Kit is highly efficient at removing inhibitors while still decreasing sample processing time. The QIAamp PowerFecal Pro DNA Kits also showed the highest A260/A280 ratios, near 1.8, of any other commercially available kit, indicating the absence of inhibitors. Less variability was also observed between the samples processed with QIAamp PowerFecal Pro DNA Kits (See: ‘Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.’). Researchers using the QIAamp PowerFecal Pro DNA Kit can identify a greater bacterial diversity with increased OTUs from stool samples compared with the first-generation QIAamp PowerFecal Kit and other commercially available kits (See: ‘Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.’).

Procedure

Samples processed with the QIAamp PowerFecal Pro DNA Kit are added to a bead beating tube. Rapid and thoroughhomogenization occurs using mechanical and chemical methods. Once cells are lysed, our patented IRT is used to removeinhibitors. Total genomic DNA is captured on a silica membrane in a spin-column format. DNA is then washed and eluted,ready for downstream applications.

Specifications

Features
Specifications
Sample typesStool, gut material, biosolids, soil

Product Resources

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Webinars (1)
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Novel solutions for unbiased results in metagenomic analysis of human microbiome samples
This webinar will focus on novel techniques for obtaining unbiased results in 16S rRNA gene sequencing of challenging microbiome samples.
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Brochures & Guides (2)
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QIAamp PowerFecal Pro DNA Kit Digital Product Profile
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QIAamp PowerFecal Pro DNA Kit Product Profile
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Kit Handbooks (1)
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QIAamp PowerFecal Pro DNA Kit Handbook
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Quick-Start Protocols (1)
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QIAamp PowerFecal Pro DNA Kit QSP
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Safety Data Sheets (1)
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MSDS QIAamp PowerFecal ProDNA Kit (50)
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。