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当前位置: 首页 > 产品中心 > peptide > Qiagen/EZ1 DNA Blood 200 µl Kit/For 48 preps: Reagent Cartridge (Blood 200 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)/951034
商品详细Qiagen/EZ1 DNA Blood 200 µl Kit/For 48 preps: Reagent Cartridge (Blood 200 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)/951034
Qiagen/EZ1 DNA Blood 200 µl Kit/For 48 preps: Reagent Cartridge (Blood 200 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)/951034
Qiagen/EZ1 DNA Blood 200 µl Kit/For 48 preps: Reagent Cartridge (Blood 200 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)/951034
商品编号: 951034
品牌: Qiagen
市场价: ¥8180.00
美元价: 4908.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

EZ1 DNA Blood 200 µl Kit

Product picture
For automated purification of DNA from 1–6 samples or 1–14 samples per run
  • Rapid purification of up to 6 µg high-quality DNA
  • Processing of up to 200 µl whole blood samples
  • Easy protocol setup with credit-card ease of use
  • Standardized processing ensures reproducible results

The EZ1 DNA Blood 200 μl Kit contains all required reagents and labware for rapid, automated purification of DNA from blood samples of up to 200 μl using magnetic particle technology. The kit can be used with the EZ1 Advanced XL(1–14 samples per run), EZ1 Advanced (1–6 samples per run),and BioRobot EZ1 (1–6 samples per run) instruments. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the instrument.

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Cat No./ID:951034
EZ1 DNA Blood 200 µl Kit (48)
$409.00
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For 48 preps: Reagent Cartridge (Blood 200µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2ml), Elution Tubes (1.5ml)
The EZ1 DNA Blood 200 µl Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

9
High-quality DNA for sensitive and specific analyses.
PCR of the single-copy MECL-1 gene was performed using DNA purified from whole blood. Genomic DNA was purified from samples of ACD-, EDTA-, and heparin-preserved human whole blood using the EZ1 DNA Blood 200 μl Kit. Template DNA was serially diluted. An aliquot of purified DNA (volume as indicated) was used in each 50 μl PCR.
9
High-quality DNA from whole blood.
DNA was purified from 200 µl blood samples using the EZ1 DNA Blood 200 µl Kit (upper lanes) and from 350 µl blood samples using the EZ1 DNA Blood 350 µl Kit (lower lanes). Lanes 1–6 and 7–12 are from the first and last of 8 processing runs on the EZ1 instrument, respectively. M: 1000 bp DNA ladder (100 ng); +: positive control; -: negative control. 2 µl (1%) of each eluate was visualized on the agarose gel.
Performance
The automated procedure provides high repeatability (see figure "High-quality DNA from whole blood") and linear yields over a range of starting amounts. DNA purified using the EZ1 DNA Blood 200 µl Kit gives high-performance results in downstream applications, such as PCR (see figure "High-quality DNA for sensitive and specific analyses").
Principle

Automated purification with the EZ1 DNA Blood 200 µl Kit requires use of the EZ1 Advanced XL and the EZ1 Advanced XL DNA Blood Card, or the EZ1 Advanced and the EZ1 Advanced DNA Blood Card, or the BioRobot EZ1 and the EZ1 DNA Blood Card. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure. Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–14 samples (EZ1 Advanced XL) or 1–6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.

Procedure

Whole blood samples (200 µl) are loaded onto the instrument, and genomic DNA is purified in one step following the automated EZ1 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl, or 50 µl volumes, ready to use in all downstream applications, such as PCR.

Applications

The high-quality DNA obtained using the EZ1 DNA Blood 200 μl Kit is suited for use in many applications, such as:

  • Population genetics and pharmacogenomic research
  • PCR and real-time PCR
  • Genotyping or sequencing
  • Oncology research
  • SNP, STR, VNTR, RAPD, NASBA, and AFLP technologies

Specifications

Features
Specifications
ApplicationsPCR, real-time RT-PCR, genotyping
Elution volume200 µl
For automated processingBioRobot EZ1
FormatSample tube
Main sample typeWhole blood
ProcessingAutomated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinDNA
Sample amount200 µl
TechnologyMagnetic particles
Time per run or per prep15–20 minutes
Yield2.6–6 µg

Product Resources

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Brochures & Guides (1)
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(EN) - EZ1 Advanced Automated Solutions — Pure Convenience
Fully automated low- to medium-throughput purification of nucleic acids
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FAQs (7)
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How do I safely inactivate biohazardous flow-through material?
FAQ ID -12
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Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
FAQ ID -1169
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Is training of lab personnel included with the purchase of a BioRobot EZ1?
FAQ ID -1239
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Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
FAQ ID -1240
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How can we get our BioRobot EZ1 upgraded when new protocols are launched?
FAQ ID -1241
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Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
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What disposables are required for nucleic acid isolations on the BioRobot EZ1?
FAQ ID -1246
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Kit Handbooks (1)
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EZ1 DNA Blood Handbook - (EN)
EZ1 DNA Blood 200 μl KitEZ1 DNA Blood 350 μl KitFor automated purification of DNA from blood and buffy coat using EZ1 instruments
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Supplementary Protocols (1)
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Purification of genomic DNA from whole blood, optimized for use in MRC-Holland MLPA® assays, using EZ1® DNA Blood Kits - (EN)
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Quick-Start Protocols (1)
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EZ1 DNA Blood Kit Quick-Start Protocol (EN)
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Safety Data Sheets (2)
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MSDS EZ1 DNA Blood 200 µl Kit (48)
CofA
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。