QIAamp DNA Blood Maxi Kit
For purification of up to 600 µg genomic, mitochondrial, or viral DNA from blood
- Rapid purification of high-quality, ready-to-use DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
The QIAamp DNA Blood Maxi Kit provides silica-membrane-based DNA purification for labs that process whole blood, plasma, serum, and other body fluids. The QIAamp DNA Blood Maxi Kit is designed for processing 3–10 ml fresh or frozen human whole blood. The QIAamp Maxi spin columns can be easily processed in a centrifuge or on vacuum manifolds (Additional Buffer AW1 [Cat. no. 19081] and Buffer AW2 [Cat. no. 19072] are required for use with the vacuum manifolds).
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Cat No./ID:51192 QIAamp DNA Blood Maxi Kit (10) $235.00 For 10 DNA maxipreps: 10 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml) |
Cat No./ID:51194 QIAamp DNA Blood Maxi Kit (50) $990.00 For 50 DNA maxipreps: 50 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml) |
Product Details
QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").
QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table).
| Leukocytes per ml | DNA yield (µg) | DNA yield (µg) |
|---|---|---|
| 2.5 x 105 | 15.8 | 95.8 |
| 1.0 x 106 | 60.4 | 91.5 |
| 5.0 x 106 | 312.0 | 94.5 |
| 1.0 x 107 | 624.6 | 94.6 |
The QIAamp DNA Blood Maxi Kit simplifies isolation of DNA from blood and related body fluids with a fast spin-column procedure (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as EDTA, citrate, or heparin, may be processed. The QIAamp DNA Blood Maxi Kit processes sample sizes of 3–10 ml, with a preparation time of 55 minutes. The typical yield from 10 ml healthy whole blood is 300–600 µg, with an elution volume of 500–2000 µl. The QIAamp DNA Blood Kits are supplied with a comprehensive handbook containing protocols for DNA isolation from a number of sample sources. QIAamp sample preparation technology is fully licensed.
The QIAamp DNA Blood Maxi Kit provides proven QIAamp technology for purification of DNA from a variatey of materials using Maxi spin columns with 50 ml collection tubes. Sample sources include:
Specifications
Features | Specifications |
| Applications | PCR, blotting |
| Elution volume | 500–2000 µl |
| Format | Spin column |
| Main sample type | Whole blood, body fluids |
| Processing | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA, viral DNA |
| Sample amount | 3–10 ml |
| Technology | Silica technology |
| Time per run or per prep | 55 minutes |
| Yield | 300–600 µg |
Product Resources
Product citations in scientific articles
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