Qiagen/QIAprep 96 Plus Kits/For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available f
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商品介绍
QIAprep 96 Plus Kits
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For purification of up to 50 μg high-quality plasmid DNA in 96-well format
- Consistently high yields of up to 50 μg
- Pure preps suitable for a wide range of applications
- Vacuum, centrifugation, and automated processing options
QIAprep 96 Plus Kits enable plasmid preparation in 96-well format. Samples can be processed using QIAvac 96 and/or a centrifuge or automated on the BioRobot Universal System. Up to 50 μg of high-quality plasmid DNA (endotoxin levels <10 EU/μg DNA)can be obtained from up to 5 ml of culture.For optimal results it is recommended to use this product together with QIAvac 96.
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Cat No./ID:27291 QIAprep 96 Plus Miniprep Kit (4) $1,334.00 For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Service), or a centrifugation system suitable for 96-well blocks. |
Cat No./ID:962241 QIAprep 96 Plus BioRobot Kit (4) $1,369.00 For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; for use with the BioRobot Universal System |
QIAprep 96 Plus Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
High-purity preps and outstanding yields
QIAprep 96 Plus Kits provide a novel method for preparation of highly pure DNA (see figure "Superior plasmid DNA quality"). Yields of up to 50 μg can be consistently achieved using an optimized procedure. The unique binding buffer provided with the kits ensures very specific binding conditions (see figures "High yields of plasmid DNA" and "Superior performance compared to other suppliers").
Principle
The unique kit chemistry and design of the Plasmid Plus 96 plates allow high binding capacities. After neutralization, a TurboFilter 96 plate is used for lysate clearing. To optimize plasmid DNA binding to the membrane of the Plasmid Plus 96 plate, a nonchaotropic binding buffer is added to the cleared lysate. The unique binding buffer ensures very specific binding conditions, resulting in DNA quality that is comparable to anion-exchange preps (see figure "Superior plasmid DNA quality"). Due to unique kit chemistry, up to 50 μg plasmid DNA per well can be obtained from a 5 ml E. coli culture (see figures "High yields of plasmid DNA" and "Superior performance compared to other suppliers").
Procedure
QIAprep 96 Plus Kits allow plasmid preparation in 96-well format.The QIAprep 96 Plus Miniprep protocol is based on a modified alkaline lysis procedure. Following lysate clearing, a simple bind-wash-elute procedure results in highly concentrated plasmid DNA, ready for direct use in subsequent applications (see flowchart "QIAprep 96 Plus Miniprep procedure").
Convenient and flexible processing
The QIAprep 96 Plus Miniprep Kit can be conveniently processed using theQIAvac 96 and/or a centrifuge, while preps can be automated on the BioRobot Universal Systemusing the QIAprep 96 Plus BioRobot Kit. Optimized protocols ensure easy and straightforwardplasmid DNA preparation in 96-well format.
Applications
Plasmid DNA purified using QIAprep 96 Plus Kits is highly suitable for a wide range of applications, including:
- Preparation of short hairpin vectors (sh-vectors)
- Enzymatic modifications
- Cloning
- Restriction digestion
- Sequencing
Specifications
Features | Specifications |
| Applications | Preparation of short hairpin vectors (sh-vectors), enzymatic modifications, cloning, restriction digestion, sequencing, etc |
| Culture volume/starting material | 1.25–5 ml culture volume |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing | QIAvac 96, centrifuge, automated |
| Samples per run (throughput) | 48–96 samples per run |
| Technology | Nonchaotropic binding chemistry |
| Time per run or prep per run | 40 minutes |
| Yield | 15–50 µg |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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