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当前位置: 首页 > 产品中心 > peptide > Qiagen/QIAxpert System/25一次性微流控载玻片,用于在QIAxpert/9002340上每次分析多达16个样品
商品详细Qiagen/QIAxpert System/25一次性微流控载玻片,用于在QIAxpert/9002340上每次分析多达16个样品
Qiagen/QIAxpert System/25一次性微流控载玻片,用于在QIAxpert/9002340上每次分析多达16个样品
Qiagen/QIAxpert System/25一次性微流控载玻片,用于在QIAxpert/9002340上每次分析多达16个样品
商品编号: 9002340
品牌: Qiagen
市场价: ¥3280.00
美元价: 1968.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

QIAxpert System

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For accelerated DNA, RNA, and protein quantification and quality control
  • Quantify nucleic acids from up to 16 samples in less than 2 minutes
  • Discriminate between molecules of interest using unique spectral protocols
  • Determine specific amounts of DNA, RNA, and other contaminating fractions
  • Rapidly perform analyses via the intuitive, full-color integrated touchscreen
  • Generate comprehensive reports to view on any computer or smart device
QIAxpert is an innovative high-speed microfluidic UV/VIS spectrophotometer which profiles sample content to differentiate between DNA and RNA and sample impurities. QIAxpert is fast and easy to use, analyzing up to 16 samples within 2 minutes — just pipet samples onto the QIAxpert slide, place it into the reader, and select the pre-programmed method on the integrated touchscreen. QIAxpert is the right tool for any fast-paced lab performing nucleic acid quantification and quality control.
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Cat No./ID:9002340
QIAxpert Instrument
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QIAxpert instrument with 1 year warranty coverage including parts, labor, and shipping; repair by sending to a regional repair center
Cat No./ID:9002360
QIAxpert System Basic 2 Year Package
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QIAxpert instrument with installation and startup training and 2 years warranty coverage including parts, labor, and shipping; repair by sending to a regional repair center
Cat No./ID:9002361
QIAxpert System Basic 3 Year Package
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QIAxpert instrument with installation and startup training and 3 years warranty coverage including parts, labor, and shipping; repair by sending to a regional repair center
Cat No./ID:9002364
QIAxpert System Full 2 Year Package
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QIAxpert instrument with installation and startup training and 2 years warranty coverage including parts, labor, and shipping; repair by sending to a regional repair center; loaner system provided within 2-3 days
Cat No./ID:9002365
QIAxpert System Full 3 Year Package
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QIAxpert instrument with installation and startup training and 3 years warranty coverage including parts, labor, and shipping; repair by sending to a regional repair center; loaner system provided within 2-3 days
Cat No./ID:9002368
QIAxpert System
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QIAxpert instrument with installation and startup training and 1 year warranty coverage including parts, labor, and shipping; repair by sending to a regional repair center
Cat No./ID:990700
QIAxpert Slide-40 (25)
$164.00
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25 disposable microfluidic slides for analyzing up to 16 samples per run on the QIAxpert
Cat No./ID:990701
QIAxpert Potassium Dichromate (3)
$350.00
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Solution for testing the photometric accuracy of the QIAxpert instrument; 3 x 1 ml vials
The QIAxpert System is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

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Flexible report options to meet your needs.
Comprehensive reports of measurements are available in several formats for data analysis and sharing.
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Microfluidic features of the QIAxpert Slide.
Expert design of the QIAxpert Slide ensures clean sample loading, flexible use of sample positions, and preservation of samples for up to 2 hours.
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RNA spectral profiling analysis.
[A] A highly pure gDNA sample shows no significant contamination as evidenced by the spectrum. Only the blue spectrum for DNA is present, while those for impurities (orange), background (gray) and residue (yellow) are not. [B] An RNA sample was spiked with gDNA. The RNA is indicated by the blue absorbance line, and the spiked DNA is represented by the orange absorbance line. [C] An RNA sample was spiked with phenol. The RNA is indicated by the blue absorbance line, and the spiked phenol is represented by the orange absorbance line. [D] A gDNA sample was spiked with hemoglobin. The gDNA is indicated by the blue absorbance line, and the spiked hemoglobin is represented by the gray absorbance line. The black absorbance curve indicates the total measured, background-corrected spectrum of the sample; the sum of DNA and RNA is shown as a pink absorbance curve (shown only if no differentiation between DNA and RNA can be performed); the blue absorbance line indicates the target molecule (DNA or RNA; app-dependent); the orange spectrum indicates impurities (DNA or RNA, salts, phenol; app-dependent); the gray absorbance curve shows the measured background of the sample (turbidity, beads, hemoglobin, chlorophyll; app-dependent); the yellow line indicates the unknown part of a sample that cannot be assigned to the above mentioned spectral parts.
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Exceptional linearity of QIAxpert.
Quantifications of gDNA on the QIAxpert are in high concordance with actual concentrations.
Performance
High system linearity for best results
QIAxpert exhibits excellent linearity and assures accurate and reliablequantifications. To assess the linearity of the system, pure gDNA was serially diluted to generate samples from 2000 to 2 ng (see figure Exceptional linearity of QIAxpert). The resulting dilutions were then measured on the QIAxpert using 2 µl for each of 5 replicates. The quantifications from the QIAxpert were plotted against the known concentration values, and the resulting plot demonstrates the exceptional linearity (with a regression coefficient of >0.999) of QIAxpert up to 2000 ng/ml or 40 OD (A260, 10 mm path length).
Proven reproducibility of measurements
To demonstrate the reproducibility of measurements with the QIAxpert, 10 ultrapure dsDNA samples were repeatedly quantified with the instrument. The sample concentrations spanned the complete dynamic range of the system and each sample was measured 10 times. Standard deviation (SD) and coefficient of variation (CV) were calculated to illustrate the minimal variability from sample to sample. Results from 6 representative samples are shown (see table below).
High reproducibility of measurements across complete dynamic range
Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6
Mean (µl/ml) 1949.4 961.6 475.9 92.7 45.0 2.7
SD 6.5 3.1 1.6 0.2 0.8 0.2
CV (%) 0.33 0.32 0.33 0.258 1.72 7.57
Principle
Flexibility and accuracy with QIAxpert Slides
QIAxpert uses disposable microfluidic slides to analyze up to 16 samples per run. On-board capillary intake and storage channels ensure easy sample loading with no cross-contamination, and allow unused positions to be used in later analyses. The capillary channels preserve samples and prevent evaporation (see figure Microfluidic features of the QIAxpert Slide). Thus, samples can be loaded and left at room temperature for up to 2 hours prior to readout. Constructed from advanced technical plastics that allow high optical transmission over the full UV/VIS spectrum, QIAxpert Slides enable highly accurate readings from just 2 µl of sample.
Smart analysis protocols based on cutting-edge bioinformatics
The QIAxpert performs classic nucleic acid and protein quantification based on absorbance measurements. In addition, smart analysis protocols called “apps” come pre-installed to quantify specific analytes of interest. Based on top bioinformatics expertise and extensive sample testing, the spectral profiling protocols let users focus on the molecule of interest, subtracting other absorbing impurities and background turbidity.
Unique spectral content profiling
To addresss challenging research samples, such as difficult-to-isolate or amplified nucleic acids, unique spectral protocols accurately quantify complex samples containing multiple UV/VIS absorbing chemicals that interfere with classical absorbance methods. Intelligent software algorithms extract from the measured UV/VIS spectrum the contributions of specific components like DNA, RNA, and impurities (see figure RNA spectral profiling analysis). The quantity of these components is then calculated. These data are very helpful to analyses such as quantitative gene expression by qPCR, where quality of purified RNA is crucial because gDNA contamination leads to decreased accuracy of results.
Procedure
It just takes a few simple steps to load samples into the QIAxpert and have the results delivered on-screen and in exportable format. Simply pipet2 µl of each sample for up to 16 samples onto a QIAxpert Slide. Once loaded, samples can be held in the microfluidic capillaries of the QIAxpert Slide for up to 2 hours. Thislets usersbatch samples and minimize the time spent at the instrument.Samples are measured immediately upon inserting the QIAxpert Slide into the instrument. Additional information and settings can be entered while QIAxpert reads the samples, thereby saving time. The readout of 16 samples is completed within 2 minutes and results are displayed directly on the integrated screen. Quantification of nucleic acids and proteins can be done by traditional absorbance readings (A260 or A280) or using unique spectral content profiling protocols that hone in on the molecule of interest.Data are easily exported to a USB stick or networked device. A report can also be printed or displayed on a smartphone or tabletby scanning a displayed QR code.
Software
Fast results with the intuitive QIAxpert Software
QIAxpert features a full-color integrated touchscreen interface, so no extra computer is required. The intuitive and easy-to-use QIAxpert Software was designed to fit a busy lab schedule. Beyond UV/VIS quantification at 260 and 280 nm wavelengths, QIAxpert Software performs spectral decomposition to selectively quantify DNA, RNA, or protein in a sample. Experiment setup is quick and easy, and can be defined while the instrument reads samples. Results are available as comprehensive data reports in HTML format that can be opened in any browser. Additionally, tabular data are available in CSV and .txt formats (see figure Flexible report options to meet your needs). To facilitate data analysis and sharing, the data are exportable to a USB stick, a networked drive, or a smart device via a QR code.
Services

Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?

If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.

Learn more about trade-in/trade-up opportunities.

Specifications

Features
Specifications
Absorbance precision0.001 OD
Absorbance accuracy4% (0.66 OD at 350 nm)
Concentration range1.5 ng/μl up to 2000 ng/μl dsDNA (A260 dsDNA application)
Data outputUSB, TCP/IP or QR code
Dimensions(W) 23 x (D) 28 x (H) 30 cm; 9 kg
Light sourceXenon flash lamp
Maximum sample residence time2 hours
Measurement range (10 mm equivalent)0.03–40 OD
Measurement timeApproximately 2 minutes for fully loaded slide
Path length0.5 mm
Optical principleUltrasensitive photodiode array spectrophotometer for UV-VIS range
Photometric range0.0005–2.0 OD
Power supply100–240 V, 50/60Hz (operating voltage 24 VDC)
Recommended sample quantity2 µl
Samples per run (throughput)1–16 samples per run (blanks included)
SoftwareOn-board software; newest software can be downloaded at www.qiagen.com/QIAxpert
TypeUV/VIS polychromatic system + reference channel
Wavelength accuracy≤0.2 nm
Wavelength range230–750 nm
Wavelength reproducibility0.1 nm
Wavelength resolution<3>

Product Resources

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Application Notes (4)
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QIAxpert Spectral Content Profiling Protocols
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QIAxpert — a powerful system for nucleic acid quality control
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QIAxpert PAXgene RNA Spectral Content Profiling App
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QIAxpert QIAsymphony Spectral Content Profiling App
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Brochures & Guides (4)
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(EN) - QIAxpert — See what"s really in your sample
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Technical Information - QIAxpert® System Quick-Start Guide
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QIAxpert Kit Compatibility List
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Technical Information (1)
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(EN) - QIAxpert Spectral Content Profiling
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Instrument User Manuals (3)
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Product Profile - QIAxpert Calibration Instructions
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QIAxpert Quick-Start Guide
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QIAxpert User Manual
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White Papers (1)
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Quality Control White Paper - A combinatorial approach to nucleic acid quality control for efficient workflow standardization and reliable data generation
This study demonstrates that the QIAxcel Advanced capillary electrophoresis system in combination with the QIAxpert UV/Vis spectrophotometer can cover the comprehensive assessment of key quality parameters of nucleic acid samples.
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Kit Handbooks (2)
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QIAxpert Slide-40 Product Sheet
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QIAxpert Potassium Dichromate Product Sheet
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(1)
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Operating Software (1)
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QIAxpert Instrument Software Version 2.4.2.88
Release of QIAxpert Instrument Software v2.4.2.88. This release includes the latest versions of the following Apps: DNA QIAamp, DNA QIAsymphony, DNA Mammalian, DNA Plant, PCR QIAquick, Purified PCR, RNA RNeasy, RNA, PAXgene RNA, RNA FFPE, A260 dsDNA, A260 RNA, A260 ssDNA, A280 Protein, UV/Vis and OD check.
For software installation instructions, please refer to the QIAxpert User Manual, chapter Tools – Upgrading the QIAxpert Software.
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Safety Data Sheets (8)
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MSDS QIAxpert Instrument
MSDS QIAxpert Slide-40 (25)
MSDS QIAxpert Potassium Dichromate (3)
MSDS QIAxpert System Basic 2 Year Package
MSDS QIAxpert System Basic 3 Year Package
MSDS QIAxpert System Full 2 Year Package
MSDS QIAxpert System Full 3 Year Package
MSDS QIAxpert, Full Agreement, no PM
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。