TissueRuptor II
- Rapid, effective disruption of a range of sample types
- Disposable probes help to minimize cross-contamination
- Time savings through use of disposable probes
- Visual monitoring of disruption using transparent probes
- Seamless integration with QIAGEN sample technologies
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- Product Details
- Specifications
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Cat No./ID:9002755 TissueRuptor II (120 V, 60 Hz, US) $982.00 Handheld rotor–stator homogenizer, 120 V, 60 Hz (for North America), 5 TissueRuptor Disposable Probes |
Cat No./ID:990890 TissueRuptor Disposable Probes (25) $351.00 25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor) |
Product Details
Genotyping, gene expression and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA and protein. The TissueRuptor II system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).
The TissueRuptorII is an integral part of QIAGEN"s complete solution for sample management— from sample collection to purification and analysis of DNA, RNA and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.
QIAGEN also provides RNAprotect TissueReagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor II.
| Analyte purified | Sample type | QIAGEN kit |
| RNA | Easy-to-lyse tissue | RNeasy Kits |
| RNeasy Plus Kits | ||
| RNeasy Protect Kits | ||
| RNA | Fiber-rich tissue | RNeasy Fibrous Tissue Kits |
| RNA | All types of tissue | RNeasy Lipid Tissue Kits |
| RNeasy Universal Tissue Kits | ||
| RNA | Plant tissue | RNeasy Plant Mini Kit |
| RNA | Yeast | RNeasy Kits |
| RNA | Bacteria | RNeasy Protect Bacteria Kits |
| microRNA | All types of tissue | miRNeasy Kits |
| DNA | Human tissue | QIAamp DNA Kits |
| DNA | Animal tissue | DNeasy Blood & Tissue Kits |
| Protein | Tissue | Qproteome Mammalian Protein Prep Kit |
| Phosphoprotein | Tissue | PhosphoProtein Purification Kit |
| Glycoprotein | Tissue | Qproteome Glycoprotein Kits |
| DNA and RNA | Tissue | AllPrep DNA/RNA Kits |
| DNA, RNA, and protein | Tissue | AllPrep DNA/RNA/Protein Mini Kit |
The TissueRuptor II is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.
After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.
The TissueRuptor II enables efficient disruption and homogenization of human, plant and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids or protein can then be performed using QIAGEN kits.
Specifications
Features | Specifications |
| Disruption principle | Simultaneous disruption and homogenization by rotation of the blade of the disposable probe |
| Features | Rapid, effective disruption of a range of sample types. Disposable probes minimize risk of cross-contamination. Time savings through use of disposable probes. Visual monitoring of disruption using transparent probes. Seamless integration with QIAGEN sample technologies. |
| Kits compatible with instrument | All Kits for purification of RNA/DNA/protein |
| Technical data | AUS/EU/UK: 230 V, 144 W, 50/60 Hz, 5000-35,000 rpm; NA: 120 V, 144 W, 50/60 Hz, 5000-35,000 rpm; Japan:100 V, 125 W, 50/60 Hz, 5000-28,000 rpm |
| Technology | Handheld rotor-stator homogenizer |
| Throughput | 1 sample per run |
| Typical run time | 15–120 seconds |
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