Qiagen/PyroMark Q48 Advanced Reagents/Reagents for 4 x 48 PyroMark Q48 Autoprep standard reactions: PyroMark Advanced Enzyme Mix, PyroMark Advanced Substrate Mix, Denaturation Solution, Annealing Buffer, Binding Buffer, Nucleotides/974002
市场价：
¥14800.00
美元价：
8880.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PyroMark Q48 Advanced Reagents
Print
For performing PyroMark Q48 Autoprep standard and long-read Pyrosequencing reactions
- Automated Pyrosequencing with integrated template preparation
- Increased throughput for more SNP and mutation assays per run
- Advanced technology, software and chemistry for long sequence runs
- Quantitative methylation analysis at consecutive CpG or CpN sites
- Improved quantification of sequence variations at any sequence position
PyroMark Q48 Advanced Reagents are designed for use with the new PyroMark Q48 Autoprep and together provide fully automated Pyrosequencing with integrated template preparation. PyroMark Q48 Advanced Reagents are highly suited for analyzing any kind of sequence variation, particularly DNA methylation at CpG or CpN sites and complex mutations, or for de novo sequencing applications such as microbial typing. For long-read Pyrosequencing runs that may require larger volumes of nucleotides, we recommend using the PyroMark Q48 Advanced CpG Reagents.
- Buy Products
- Product Details
- Service Plans
Buy Products
Cat No./ID:974002 PyroMark Q48 Advanced Reagents (4 x 48) $740.00 Reagents for 4 x 48 PyroMark Q48 Autoprep standard reactions: PyroMark Advanced Enzyme Mix, PyroMark Advanced Substrate Mix, Denaturation Solution, Annealing Buffer, Binding Buffer, Nucleotides |
Cat No./ID:974022 PyroMark Q48 Advanced CpG Reagents (4 x 48) $815.00 Reagents for 4 x 48 PyroMark Q48 Autoprep CpG and long-read reactions: PyroMark Advanced Enzyme Mix, PyroMark Advanced Substrate Mix, Denaturation Solution, Annealing Buffer, Binding Buffer, Nucleotides |
Cat No./ID:974203 PyroMark Q48 Magnetic Beads (300) $148.00 Magnetic streptavidin-coated Sepharose beads for running 300 PyroMark Q48 Autoprep reactions |
Cat No./ID:974230 PyroMark Q48 AutoPrep Starter Kit $1,295.00 PyroMark Q48 Magnetic Beads (300), PyroMark Q48 Advanced CpG Reagents (4 x 48), PyroMark Control Oligo, PyroMark Q48 Discs (50) and PyroMark Q48 Absorber Strips (100) |
PyroMark Q48 Advanced Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Advanced technology, software and chemistry for long and reliable sequence runs
PyroMark Q48 Autoprep features improved chemistry and instrument operation algorithms that significantly increase assay read length and accuracy in base calling, mutation analysis and methylation quantification compared to PyroMark Q96 and PyroMark Q24 systems. Assay read length in previous systems was limited by background peaks and reduced light signals in the sequencing reaction. The updated PyroMark “Advanced” chemistry and algorithms reduce this background, thereby increasing read length and reliability. Depending on the sequence to be analyzed, highly accurate read lengths of 140 or more bases can be obtained in just a single reaction.Increased throughput for more SNP and mutation assays per run
Multiple Primer Dispensation (MPD) is a strategy to increase the capacity of automated sequencing primer dispensation in case more assays are needed than cartridge reservoirs are available. The PyroMark Q48 Autoprep Primer Cartridge has 3 reservoirs, but if more assays are needed per disc, the primers can be mixed and filled into the same reservoir of the primer cartridge. After template preparation, primer mixes are dispensed into the wells automatically. Since only one PCR template is present in each well, only the corresponding sequencing primer will bind to the template. All other primers will stay in solution but will not bind (see The principle of multiple primer dispensation). Primers in each MPD mix should be designed and checked to avoid formation of primer–dimers or binding to another PCR template.Compatibility of assays among different PyroMark platforms
Previously designed Pyrosequencing assays are readily compatible with the new PyroMark Q48 Autoprep instrument and the “Advanced” chemistry. Data indicate that the same mutation frequencies and methylation quantification results are obtained when an assay is run on the various PyroMark platforms (see Compatibility among PyroMark platforms for methylation analysis and Compatibility among PyroMark platforms for mutation analysis). The cross-platform compatibility is also independent of the distance of the analyzed site away from the sequencing primer (see Compatibility among PyroMark platforms regardless of CpG position). This is particularly important when analyzing multiple sequence variations in a single run, which is typical for complex mutation assays or methylation analysis of consecutive CpG sites.New disc design enables magnetic template preparation without cross contamination
PyroMark Q48 Discs are specially designed to automate template preparation and Pyrosequencing in the same instrument without manual user interaction. All buffers used for template preparation are efficiently removed from the sample and the disc during the run without the risk of any cross-contamination from well-to-well of one run or from disc-to-disc between subsequent runs (see Excluding well-to-well and disc-to-disc cross-contamination).Principle
The PyroMark Q48 Autoprep uses proven real-time sequence-based Pyrosequencing technology for detection and quantification in genetic analyses and epigenetic methylation studies. The system can analyze up to 48 samples simultaneously. An easy-to-use, automated protocol prepares single-stranded DNA samples without manual interaction from the user. This protocol uses magnetic streptavidin-coated Sepharose beads (PyroMark Q48 Magnetic Beads), which bind to the biotinylated PCR strand. Annealing of sequencing primers can be automated for up to four different sequencing primers. If more sequencing primers are used, the primers can be manually added to the single-stranded DNA samples.
Procedure
A run file is created using the PyroMark Q48 Advanced software and is transferred to the instrument via a USB drive or network connection. Reagents, nucleotides and buffers are loaded into the PyroMark Q48 Autoprep cartridges, according to the volumes indicated on the touchscreen. The biotinylated PCR product and magnetic streptavidin-coated Sepharose beads are loaded into the wells of the Q48 Disc, and the disc and an absorber strip are placed into the instrument. Preparation of the single-stranded template, along with sequencing primer annealing are now completely automated and require no additional user interaction. Up to 3 separate sequencing primers or Multiple Primer Dispensation (MPD) mixes can be dispensed automatically. Optional, manual primer addition further increases the number of different assays per run.
Applications
Pyrosequencing is increasingly important for research applications in a variety of disciplines. Whether examining drug-resistance development in pathogens, the role of epigenetic DNA methylation in gene expression regulation, genetic markers for specific phenotypes in livestock or polymorphisms in forensic samples of mitochondrial DNA, PyroMark platforms enable powerful and versatile analysis of genetic and epigenetic variation. In addition, because Pyrosequencing integrates sequence detection and quantification, the enhanced analysis resolution can lead to new discoveries.
Service Plans
Cat No./ID:9244446 Pyro Q48, Full Agreement Inquire Instrument repair service for the PyroMark Q48 at regional repair center. Shipping, labor and parts included during the Full Agreement period. Loaner instrument provided within 2–3 business days. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service during the Full Agreement period. |
PyroMark Q48 Advanced Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Resources
Customers who bought these products also bought
- Cat No./ID:974912
PyroMark Q48 Absorber Strips (100)
100 absorber strips used for liquid collection during automated template preparation with PyroMark Q48 Autoprep$146.00Add To Cart - Cat No./ID:974901
PyroMark Q48 Discs (50)
50 discs for automated template preparation and Pyrosequencing reactions using a PyroMark Q48 Autoprep$190.00Add To Cart - Cat No./ID:9024326
PyroMark Q48 Software License (5)
Five additional licenses for PyroMark Q48 Software. Only valid together with PyroMark Q48 Autoprep$2,997.00Add To Cart - Cat No./ID:979203
PyroMark Control Oligo
For installation check of system$499.00Add To Cart - Cat No./ID:978703
PyroMark PCR Kit (200)
For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water.$321.00Add To Cart - Cat No./ID:978801
PyroMark OneStep RT-PCR Kit (50)
For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water$236.00Add To Cart - Cat No./ID:59826
EpiTect Fast DNA Bisulfite Kit (200)
For 200 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers$785.00Add To Cart - Cat No./ID:978705
PyroMark PCR Kit (800)
For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water$1,096.00Add To Cart - Cat No./ID:69967
TissueLyser Single-Bead Dispenser, 7 mm
For dispensing individual beads (7 mm diameter)$261.00Add To Cart - Cat No./ID:929500
QX Intensity Calibration Marker (1 ml)
1 ml QX Intensity Calibration Marker$81.40Add To Cart - Cat No./ID:978746
PyroMark CpG Assay (200)
PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)Select Targets - Cat No./ID:387416
Investigator Quantiplex Pro Calibration Kit
For use on Applied Biosystems 7500 Real-Time Systems: Calibration Standard FAM (60 µl), Calibration Standard JOE (60 µl), Calibration Standard ATTO 550 (60 µl), Calibration Standard ROX (60 µl), Calibration Standard ATTO 647N (60 µl), Quantiplex Pro Calibration Buffer (30 ml)
$332.00Add To Cart - Cat No./ID:9018956
Reagent Block, 24x2ml Flat-Base Tubes
Reagent Block; intended to hold reagents and standards on the instrument worktable; holds 24 x 2 ml Flat-Base Tubes$413.00Add To Cart - Cat No./ID:59824
EpiTect Fast DNA Bisulfite Kit (50)
For 50 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers$229.00Add To Cart - Cat No./ID:382617
Investigator 26plex QS Kit (400)
Primer Mix, Fast Reaction Mix 3.0includingTaqDNA Polymerase, Control DNA, allelic ladder 26plex, and nuclease-free water
$8,908.00Add To Cart - Cat No./ID:59695
EpiTect PCR Control DNA Set (100)
Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs$432.00Add To Cart - Cat No./ID:978776
PyroMark Custom Assay (200)
User-designed PCR and sequencing primers for Pyrosequencing analysis (200 reactions; tube format)Select Targets - Cat No./ID:204256
QuantiFast Probe PCR Kit (2000)
For 2000 x 25 µl reactions: 25 ml 2x QuantiFast Probe PCR Master Mix (contains ROX dye), 20 ml RNase-Free Water$2,033.00Add To Cart - Cat No./ID:85600
TissueLyser LT
Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)$6,110.00Add To Cart - Cat No./ID:69980
TissueLyser LT Adapter, 12-Tube
Adapter for disruption of up to 12 samples in 2 ml microcentrifuge tubes on the TissueLyser LT$1,230.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
