Qiagen/Epiect对照DNA和对照DNA集/人类对照DNA集（包含亚硫酸氢盐转化的甲基化和非甲基化DNA以及未转化的非甲基化DNA），用于100个对照PCR/59695
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¥8640.00
美元价：
5184.00
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3392242852
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4000-520-616
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info@ebiomall.com
商品介绍
EpiTect Control DNA and Control DNA Set
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For control experiments during methylation analysis
- Convenient, ready-to-use, quality-controlled DNA
- Bisulfite-converted DNA for control experiments
- Suitable for all methylation analyses
EpiTect Control DNAs are ready-to-use, completely methylated or completely unmethylated bisulfite converted DNAs, and untreated, unmethylated genomic DNA, for standardized and reliable control reactions for methylation analysis. The methylated, bisulfite converted DNA as well as unmethylated, bisulfite converted and unconverted DNA are stored in EB Buffer (10 mM Tris Cl) in a ready-to-use solution.
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Cat No./ID:59695 EpiTect PCR Control DNA Set (100) $432.00 Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs |
EpiTect Control DNA and Control DNA Set are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
EpiTect Control DNAs are highly suited for use as quantification standards for probe-based real-time PCR methylation analysis (see figure "Real-time PCR standards") and high-resolution melting (HRM) methylation analysis (see figure "HRM quantification standards").
Principle
The EpiTect Control DNA Set contains all control DNAs needed for standardized and reliable methylation control reactions in a ready-to-use kit format . In methylation-specific PCR, for example, control reactions must be carried out to ensure that PCR probes and primers bind specifically to methylated or unmethylated DNA (see figure Use of EpiTect Control DNA in PCR for methylation analysis). These reactions require various concentrations of completely bisulfite converted control DNA that is fully methylated or fully unmethylated (see figuresEpiTect Control DNA Pyrogram and "EpiTYPER MALDI-TOF analysis of WBA DNA"). Additionally, mixtures of these control DNAs can serve as quantification standards when determining degree of methylation in HRM and probe-based methylation experiments.
Procedure
Standardized workflows in epigenetics
Accessing epigenetic information is of prime importance for many areas of biological and medical research — particularly oncology, but also stem cell research and developmental biology. However, the analysis of changes in DNA methylation is challenging, due to the lack of standardized methods for providing reproducible data particularly from limited sample material. With its newly introduced EpiTect solutions, QIAGEN makes available standardized, pre-analytical and analytical solutions from DNA sample collection, stabilization and purification, to bisulfite conversion and real-time or end-point PCR methylation analysis or sequencing (see figure "Standardized workflows in epigenetics").
Applications
EpiTect Control DNAs are suitable for use in control reactions for all types of methylation analyses, including:
- Evaluating primer specificity for MSP
- Quantification standard for HRM and MethyLight PCR
- Evaluating primer and probe specificity for MethyLight PCR
- Checking efficiency of bisulfite conversion reactions
Specifications
Features | Specifications |
| Applications | End point Methylation Specific PCR (MSP), real-time methylation specific PCR |
| Concentration | 10 ng/µl (1 µg in total) excepted the unmethylated control DNA: 50 ng/µl (10 µg in total) |
| Number of reactions | for 1000 control PCRs |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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