4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类化合物
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > peptide > Qiagen/PyroMark缓冲液和溶液/用于生物素化PCR产物与Sepharose珠的结合/979006
商品详细Qiagen/PyroMark缓冲液和溶液/用于生物素化PCR产物与Sepharose珠的结合/979006
Qiagen/PyroMark缓冲液和溶液/用于生物素化PCR产物与Sepharose珠的结合/979006
Qiagen/PyroMark缓冲液和溶液/用于生物素化PCR产物与Sepharose珠的结合/979006
商品编号: 979006
品牌: Qiagen
市场价: ¥1174.00
美元价: 704.40
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

PyroMark Buffers and Solutions

Product picture

For optimal preparation and analysis of template DNA by Pyrosequencing

    Generating Pyrosequencing templates from PCR amplicons is fast and straightforward with the PyroMark vacuum workstation. These buffers and solutions are specifically designed for this purpose. PyroMark Binding Buffer facilitates binding of biotinylated PCR amplicons to streptavidin-coated Sepharose beads. These beads are then immobilized with the vacuum tool of the workstation. PyroMark Denaturation Solution gently separates the complementary strand from the biotin-tagged strand destined to become the Pyrosequencing template, and PyroMark Wash Buffer ensures that only the biotin-tagged, single-stranded DNA remains immobilized to the vacuum tool. Finally, when the single-stranded DNA is released into reaction vessels containing the Pyrosequencing primer, hybridization of primer and template is enhanced by PyroMark Annealing Buffer.

    • Buy Products
    • Product Resources

    Buy Products

    Cat No./ID:979006
    PyroMark Binding Buffer (200 ml)
    $58.70
    Order Product
    For binding of biotinylated PCR product to Sepharose beads
    Cat No./ID:979007
    PyroMark Denaturation Sol. (500 ml)
    $58.70
    Order Product
    For denaturation of double-stranded PCR product intosingle-stranded DNA template
    Cat No./ID:979008
    PyroMark Wash Buffer (conc., 200 ml)
    $58.40
    Order Product
    For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA
    Cat No./ID:979009
    PyroMark Annealing Buffer
    $58.80
    Order Product
    For annealing of sequencing primer to DNA template
    PyroMark Buffers and Solutions are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

    Product Resources

    You are not authorized to download the resource

    FAQs (12)
    • Sort options
      • Sort alphabetically
    What concentration should be used for the sequencing primer in pyrosequencing?
    FAQ ID -2826
    View
    Can I order the nucleotides from PyroMark Gold Reagents separately?
    FAQ ID -2827
    View
    Which purity grade is recommended for pyrosequencing primers?
    FAQ ID -2832
    View
    How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations?
    FAQ ID -2848
    View
    Where can I order the Streptavidin Sepharose beads for pyrosequencing?
    FAQ ID -2850
    View
    How many nucleotides of a homopolymer can be resolved in pyrosequencing?
    FAQ ID -2871
    View
    Can unused wells in a pyrosequencing plate be used in the next run?
    FAQ ID -2872
    View
    What is the reason for signals ceasing in the middle of a pyrosequencing run?
    FAQ ID -2875
    View
    How do I reduce background peaks in the pyrosequencing pyrogram?
    FAQ ID -2877
    View
    How do I prevent a drifting baseline in my pyrosequencing pyrogram?
    FAQ ID -2878
    View
    What is the reason for a high substrate peak in the pyrosequencing pyrogram?
    FAQ ID -2879
    View
    What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram?
    FAQ ID -2881
    View
    User-Developed Protocols (1)
    • Sort options
      • Sort alphabetically
    Pyrosequencing® method for the forensic identification of tissue source using QIAGEN’s PyroMark® Q24 Advanced system - (EN)
    Show details
    Safety Data Sheets (4)
    • Sort options
      • Sort alphabetically
    MSDS PyroMark Annealing Buffer
    MSDS PyroMark Binding Buffer (200 ml)
    MSDS PyroMark Denaturation Sol. (500 ml)
    MSDS PyroMark Wash Buffer (conc., 200 ml)
    fragment fix placeholder

    Customers who bought these products also bought

    • Cat No./ID:972804
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-id-accessories-and-reagents/

      PyroMark Gold Q96 Reagents (5 x 96)

      For performing Pyrosequencing reactions on the PyroMark Q96 ID (5 x 96) and PyroMark Q96 MD (15 x 96)
      $601.00
      Add To Cart
    • Cat No./ID:970802
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q24-accessories-and-reagents/

      PyroMark Gold Q24 Reagents (5 x 24)

      Nucleotides, enzyme, and substrate solutions, intended for use with PyroMark Q24
      $376.00
      Add To Cart
    • Cat No./ID:979202
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q24-advanced-accessories-and-reagents/

      PyroMark Q24 Cartridge (3)

      Reusable dispensing tool for nucleotide and reagent delivery, for use with PyroMark Q24 and PyroMark Q24 Advanced
      $515.00
      Add To Cart
    • Cat No./ID:979004
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-id-accessories-and-reagents/

      PyroMark Q96 Cartridge (3)

      For delivery of nucleotides and reagents for use with PyroMark Q96 ID
      $515.00
      Add To Cart
    • Cat No./ID:979002
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-id-accessories-and-reagents/

      PyroMark Q96 Plate Low (100)

      For sample analysis of DNA template on PyroMark Q96 ID
      $249.00
      Add To Cart
    • Cat No./ID:978703
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/assays/pyromark-pcr-kit/

      PyroMark PCR Kit (200)

      For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water.
      $321.00
      Add To Cart
    • Cat No./ID:979201
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q24-advanced-accessories-and-reagents/

      PyroMark Q24 Plate (100)

      24-well plate for samples, for use with PyroMark Q24 and PyroMark Q24 Advanced; 100 plates in each package
      $249.00
      Add To Cart
    • Cat No./ID:978705
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/assays/pyromark-pcr-kit/

      PyroMark PCR Kit (800)

      For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water
      $1,096.00
      Add To Cart
    • Cat No./ID:970922
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q24-advanced-accessories-and-reagents/

      PyroMark Q24 Advanced CpG Reagents (4 x 24)

      Enzyme mix, substrate mix, nucleotides, and buffers for CpG and long read Pyrosequencing assays; intended for use with PyroMark Q24 Advanced
      $400.00
      Add To Cart
    • Cat No./ID:979010
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q24-advanced-accessories-and-reagents/

      PyroMark Vacuum Prep Filter Probe (100)

      Reusable filter probes for PyroMark Vacuum Workstation Q96 and Q24
      $343.00
      Add To Cart
    • Cat No./ID:972807
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-md-accessories-and-reagents/

      PyroMark Gold Q96 Reagents (50 x 96)

      For performing Pyrosequencing reactions on the PyroMark Q96 MD
      $1,653.00
      Add To Cart
    • Cat No./ID:979101
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-md-accessories-and-reagents/

      PyroMark Q96 HS Plate (100)

      For sample analysis of DNA template on PyroMark Q96 MD
      $872.00
      Add To Cart
    • Cat No./ID:979104
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-md-accessories-and-reagents/

      PyroMark Q96 HS Capillary Tip (8)

      Reusable capillary tips (8 in each package); for dispensing nucleotides (CDTs); for use with PyroMark Q96 MD
      $343.00
      Add To Cart
    • Cat No./ID:970032
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/assays/pyromark-q24-tests/

      PyroMark Q24 CpG MGMT (4 x 24)

      Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +17 to +39 of exon 1 of the MGMT gene
      $766.00
      Add To Cart
    • Cat No./ID:979102
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-md-accessories-and-reagents/

      PyroMark Q96 HS Reagent Tip (4)

      Reusable tips (4 in each package); for dispensing reagents (RDTs); for use with PyroMark Q96 MD
      $346.00
      Add To Cart
    • Cat No./ID:979206
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q24-advanced-accessories-and-reagents/

      PyroMark Q24 Vacuum Prep Troughs (12)

      Reusable plastic troughs for use with all PyroMark Q24 Vacuum Workstations
      $48.80
      Add To Cart
    • Cat No./ID:979011
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-id-accessories-and-reagents/

      PyroMark Q96 Vacuum Prep Trough (5)

      Reusable plastic troughs for use with all PyroMark Q96 Vacuum Workstations
      $48.80
      Add To Cart
    • Cat No./ID:972812
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-id-accessories-and-reagents/

      PyroMark Gold Q96 SQA Reagents (1x96)

      For performing Pyrosequencing reactions on the PyroMark Q96 ID
      $242.00
      Add To Cart
    • Cat No./ID:970042
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/assays/pyromark-q24-tests/

      PyroMark Q24 CpG LINE-1 (4 x 24)

      Methylation detection assay: PCR primers and sequencing primer for quantification of global methylation level in transposable elements
      $790.00
      Add To Cart
    • Cat No./ID:972824
      /us/products//discovery-and-translational-research/epigenetics/dna-methylation/pyrosequencing/consumables/pyromark-q96-md-accessories-and-reagents/

      PyroMark Gold Q96 CDT Reagents (6x96)

      For performing Pyrosequencing reactions on the PyroMark Q96 MD in combination with the capillary dispensing tips (CDT)
      $289.00
      Add To Cart
    品牌介绍
    QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。