Qiagen/PyroMark Q24 Advanced Reagents/Enzyme mix, substrate mix, nucleotides, and buffers for Pyrosequencing assays; intended for use with PyroMark Q24 Advanced/970902
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¥7520.00
美元价：
4512.00
联系Q Q：
3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
PyroMark Q24 Advanced Reagents
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For advanced methylation and mutation quantification in long sequence runs using Pyrosequencing
- Advanced technology, software, and chemistry for long sequence runs
- Quantitative methylation analysis at consecutive CpG or CpN sites
- Improved quantification of sequence variations at any sequence position
PyroMark Q24 Advanced Reagents feature advanced Pyrosequencing chemistry toprovideeven better real-time sequence-based detection and quantification than before. PyroMark Q24 Advanced Reagents are optimized for use with PyroMark Q24 Advanced Systems and arehighly suited for analyzing any kind of sequence variation, particularly DNA methylation at CpG or CpN sites, complex mutations, or for de novo sequencing applications such as microbial typing. For long-read Pyrosequencing runs which may require larger volumes of nucleotides, such as de novo sequencing or methylation analyses we recommend using the PyroMark Q24 Advanced CpG Reagents.
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Cat No./ID:970902 PyroMark Q24 Advanced Reagents (4 x 24) $376.00 Enzyme mix, substrate mix, nucleotides, and buffers for Pyrosequencing assays; intended for use with PyroMark Q24 Advanced |
Cat No./ID:970922 PyroMark Q24 Advanced CpG Reagents (4 x 24) $400.00 Enzyme mix, substrate mix, nucleotides, and buffers for CpG and long read Pyrosequencing assays; intended for use with PyroMark Q24 Advanced |
PyroMark Q24 Advanced Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Longer Pyrosequencing runs and improved accuracy
PyroMark Q24 Advanced features improved chemistry and instrument operation algorithms that significantly increase the assay read length and accuracy of the base calling functionality, enabling easy analysis of long de novo sequencing runs. Assay read length was previously limited by background peaks and reduced light signals in the sequencing reaction. The updated chemistry and algorithms of PyroMark Q24 Advanced reduce these background peaks, thereby increasing read length and reliability. Depending on the sequence to be analyzed, highly accurate read lengths of 140 or more bases can be obtained in just a single PyroMark Q24 Advanced reaction (see figure Long de novo sequencing runs).Improved methylation analysis at any position
PyroMark Q24 Advanced enables improved methylation quantification in long sequence runs at any sequence position. Previously, analysis of methylation sites further away from the sequencing primer could be uncertain, but now with longer read lengths and higher accuracy, methylation quantification is highly reliable throughout the entire sequencing run (see figure Analysis of 16 CpG sites in a long sequence run).Improved sequencing accuracy in homopolymers
Bisulfite conversion in DNA methylation analysis leads to frequent poly T stretches in the nucleotide sequence, and analysis of CpG sites directly after such T homopolymers has previously been challenging due to uncertain quantification of the light signal at these sites. The increased accuracy of PyroMark Q24 Advanced enables reliable quantification of CpG methylation behind and even within a stretch of 8 T nucleotides (see figure Improved methylation quantification in homopolymers).Analysis of mutations over long sequences
PyroMark Q24 Advanced also provides reliable quantification of multiple polymorphisms in a single assay. Since single nucleotide polymorphisms (SNPs) and other mutations are often not located close to one another, traditional Pyrosequencing chemistry usually requires separate assays for each mutation site to be analyzed. The new chemistry of PyroMark Q24 Advanced allows much longer runs, enabling reliable analysis of more than one mutation or SNP in the same run (see figure Quantitative mutation analysis in long sequence runs).Principle
Pyrosequencing technology, which is based on the principle of sequencing by synthesis, provides quantitative data in sequence context within minutes. PyroMark Q24 Advanced Reagents and PyroMark Q24 Advanced CpG Reagents feature updated Pyrosequencing chemistry to enable analysis ofcomplex mutations, methylation analysis in epigenetic research,resistance typing, and microbial identification. These reagents are optimized for use with PyroMark Q24 Advanced Systems.
Procedure
Fast and easy sample preparation
Prior to Pyrosequencing, a biotinylated PCR product is generated. This biotinylated PCR product is bound to Streptavidin-coated Sepharose beads, and the beads are captured with the Vacuum Tool on the Vacuum Workstation, where they are thoroughly washed and subsequently denatured, generating single-stranded DNA suitable for Pyrosequencing. This template DNA is released into the Pyrosequencing reaction plate containing the sequencing primer, and after primer annealing, the plate is placed into the PyroMark instrument. PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents contain the enzymes, nucleotides, and substrate for the Pyrosequencing reaction; these are pipetted into the dispensing cartridge, according to the volumes provided by the software, and are also placed into the instrument for the Pyrosequencing run.Applications
Pyrosequencing is becoming increasingly important for research applications in a variety of disciplines. Whether examining drug-resistance development in pathogens, the role of epigenetic DNA methylation in gene expression regulation, genetic markers for specific phenotypes in livestock, or polymorphisms in forensic samples of mitochondrial DNA, the PyroMark Q24 Advanced enables powerful and versatile analysis of genetic and epigenetic variation. In addition, because Pyrosequencing integrates sequence detection and quantification, the enhanced analysis resolution can lead to new discoveries.
Important:PyroMark Q24 Advanced Reagents and PyroMark Q24 Advanced CpG Reagents cannot be used with PyroMark Q24 Software. PyroMark Gold Q24 Reagents cannot be used with PyroMark Q24 Advanced Software. Using the incorrect reagent and software combination will result in incorrect reagent dispensations and lead to inaccurate results.
| PyroMark Q24 Advanced | PyroMark Q24 | |
|---|---|---|
| Throughput | 1–24 samples | 1–24 samples |
| Running volume | 25 µl | 25 µl |
| PCR requirements | 5–10 µl (~0.5–3 pmol of product) | 5–10 µl (~0.5–3 pmol of product) |
| Read length (assay- and sequence-dependent) | 10–140 bp or more | 10–80 bp |
| Application software | PyroMark Q24 Advanced SW (requires firmware 1.5.6903 or higher) | PyroMark Q24 SW 2.0 |
| Software functionality | SEQ (de novo sequencing) CpG/CpN methylation SNP AQ | SQA (de novo sequencing)CpG methylation AQ/SNP |
| Main applications | Complex mutation analysisEpigenetics (CpG and CpN analysis)Resistance typing and microbial ID | Mutation analysisResistance typing |
| Compatible reagents | PyroMark Q24 Advanced ReagentsPyroMark Q24 Advanced CpG Reagents | PyroMark Q24 Gold Reagents |
| Sensitivity | 2% mutation98% wt | 2% mutation98% wt |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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