Qiagen/PyroMark Q24 Tests/Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +148 to +182 in exon 1 of the gene/970012
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¥15320.00
美元价：
9192.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
PyroMark Q24 Tests
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For analyzing sequence variation or methylation of specific genes using the PyroMark Q24 Instrument
- Detection and quantification of different mutations in a single run
- Flexible software for post-run analysis of adjacent mutations
- Quantification of individual or multiple CpG sites in different genes
- Estimation of global CpG methylation in defined regions of interest
- Sequence context provides built-in control of the assay
PyroMark Q24 Tests are a range of assays suitable for highly reliable, quantitative mutation and CpG methylation analysis and are optimized for use with the PyroMark Q24. Tests designated with "Q24" are optimized for use with the PyroMark Q24, while others such as PyroMark HFE and PyroMark MTHFR can be used with the PyroMark Q96 ID and PyroMark Q96 MD as well. For more tests for these instruments, see PyroMark Q96 Tests.
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Cat No./ID:970012 PyroMark Q24 CpG p16 (4 x 24) $766.00 Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +148 to +182 in exon 1 of the gene |
Cat No./ID:970022 PyroMark Q24 CpG MLH1 (4 x 24) $766.00 Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region –209 to –181 from the transcription start site in the MLH1 gene |
Cat No./ID:970032 PyroMark Q24 CpG MGMT (4 x 24) $766.00 Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +17 to +39 of exon 1 of the MGMT gene |
Cat No./ID:970042 PyroMark Q24 CpG LINE-1 (4 x 24) $790.00 Methylation detection assay: PCR primers and sequencing primer for quantification of global methylation level in transposable elements |
Cat No./ID:972432 PyroMark MTHFR (96) Log in to see pricing For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24) |
Cat No./ID:972442 PyroMark HFE (96) Log in to see pricing For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24) |
PyroMark Q24 Tests are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Principle
PyroMark Q24 CpG LINE-1, PyroMark Q24 CpG MGMT, PyroMark Q24 CpG MLH1, and PyroMark Q24 CpG p16 enable highly reliable methylation results. In addition, a built-in control for bisulfite treatment improves accuracy and reproducibility of the results.
Procedure
Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q24 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.
Applications
PyroMark Q24 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.
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QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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