Qiagen/PyroMark Q96 Tests/For quantification of methylation level in the p16 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)/972012
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¥15320.00
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9192.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
PyroMark Q96 Tests
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For analyzing gene-specific sequence variation or methylation on PyroMark Q96 ID or PyroMark Q96 MD
- Detection and quantification of mutations in cancer-related genes
- Quantification of individual or multiple CpG sites in different genes
- Estimation of global CpG methylation in defined regions of interest
- Sequence context provides built-in quality control of the assays
PyroMark Q96 Tests are a range of predesigned assays suitable for highly reliable, quantitative analysis of mutations or SNPs, as well as CpG methylation. Tests designated specifically with "Q96" are optimized for use with the PyroMark Q96 MD and/or PyroMark Q96 ID Instruments. Other tests, such as PyroMark HFE and PyroMark MTHFR, can additionally be performed on the PyroMark Q24 (with the exception of PyroMark APOE).
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Cat No./ID:972012 PyroMark Q96 CpG p16 (96) $766.00 For quantification of methylation level in the p16 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID) |
Cat No./ID:972022 PyroMark Q96 CpG MLH1 (96) $766.00 For quantification of methylation level in promoter region of the MLH1 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID) |
Cat No./ID:972032 PyroMark Q96 CpG MGMT (96) $766.00 For quantification of methylation level in the MGMT gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID) |
Cat No./ID:972052 PyroMark Q96 CpG Prader-Willi (96) $790.00 For quantification of methylation level in Prader-Willi and Angelman Syndromes (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID) |
Cat No./ID:972422 PyroMark APOE (96) Log in to see pricing For detection of polymorphism in the APOE gene (2 x 96 reactions on PyroMark Q96 MD or 1 x 96 reactions on PyroMark Q96 ID) |
Cat No./ID:972432 PyroMark MTHFR (96) Log in to see pricing For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24) |
Cat No./ID:972442 PyroMark HFE (96) Log in to see pricing For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24) |
Cat No./ID:973043 PyroMark Q96 CpG LINE-1 (2 x 96) $778.00 For quantification of global methylation level in transposable elements; only for use with PyroMark Q96 MD |
PyroMark APOE, PyroMark HFE, PyroMark MTHFR, PyroMark Q96 CpG LINE-1, PyroMark Q96 CpG MGMT, PyroMark Q96 CpG MLH1, PyroMark Q96 CpG p16, and PyroMark Q96 CpG Prader-Willi tests are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The PyroMark Q96 Tests arehighly suitable for accurately measuring the CpG methylation status,as exemplified with the PyroMark Q96 CpG p16 Test (see figure PyroMark p16 analysis).
Principle
PyroMark Q96 Tests used in combination with PyroMark Q96 MD and/or PyroMark Q96 ID Systems enable accurate, quantitative mutation (or SNP) and methylation analysis by Pyrosequencing. The assays consist of PCR primers and a sequencing primer.
Procedure
Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q96 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.
Applications
PyroMark Q96 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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