Qiagen/PyroMark CpG分析/978746
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¥1912.00
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1147.20
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3392242852
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4000-520-616
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info@ebiomall.com
商品介绍
PyroMark CpG Assays
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Predesigned assays for quantification of CpG methylation by Pyrosequencing
- Advanced assay design algorithms provide optimized results
- Easy-to-order assays for gene-specific or methylation array CpG sites
- Comprehensive coverage of CpG sites in human, mouse, and rat genomes
- Convenient download of run files and analysis settings
- Top performance from a cost-effective solution
PyroMark CpG Assays are the only genomewide, predesigned methylation assays for Pyrosequencing analysis that use a tailored design algorithm for highly specific assay design and successful CpG methylation results. Our upgraded assay database now includes over 84,000 individual assays for gene-specific human, mouse, and rat CpG sites. We now also offer predesigned assays for validating results from methylation arrays, such as the Infinium HumanMethylation450K and MethylationEPIC BeadChip arrays.
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- Product Details
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Cat No./ID:978746 PyroMark CpG Assay (200) $95.60 PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format) |
Cat No./ID:978754 PyroMark CpG Assay 96 well (50) $63.30 PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (50 reactions; plate format); minimum order of 24 assays per plate |
Cat No./ID:978756 PyroMark CpG Assay 96 well (200) $96.20 PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; plate format); minimum order of 24 assays per plate |
PyroMark CpG Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Primer design optimized for high performance
Highly specific and unbiased amplification is especially important for methylation analysis by Pyrosequencing. Because bisulfite converted DNA is a less complex template, it can be challenging to amplify specifically and at high yields, but with predesigned PyroMark CpG Assays, this is not a problem. The PCR and sequencing primers included in PyroMark CpG Assayshave been checked against the entire human, mouse, or rat bisulfitome and are delivered at optimal concentrations for high performance. Together with the unique DNA protect technology of EpiTect Fast Bisulfite Conversion Kits and the carefully formulated reagents of the PyroMark PCR Kit, these predesigned assays ensure high yield and quality of sequencing template and increase reliability of downstream Pyrosequencing analyses. Evaluation of PCR performance for 310 randomly selected PyroMark CpG Assays showed that 305 assays (98%) produced the predicted PCR amplicon, suitable for use as Pyrosequencing template. From these assays, 281 (92%) provided successful Pyrosequencing results (see figures High PCR success rate with bisulfite converted DNA and High Pyrosequencing success rate of predesigned PyroMark CpG Assays). The high-performing assays eliminate the need for tedious assay optimization and shorten the time to reliable results.Principle
Assays for analyzing gene-specific CpG sites
PyroMark CpG Assays enable methylation analysis of specific targets across the genome. Our database now contains over 84,000 predesigned assays covering more than 80% of gene-specific CpG islands for the human, mouse, and rat genomes. These assays are designed using a carefully tailored algorithm that generates optimal assay design based on bisulfite converted DNA. As such, these fast and easy-to-order assays minimize time spent on assay optimization and maximize analysis success. PyroMark CpG Assays expand the streamlined Pyrosequencing workflow to enable methylation analysis of virtually any CpG island in the human, mouse, or rat genome (see figure Analysis of ESR1).Assays for methylation array validation
Hybridization arrays enable high-throughput discovery of variations in DNA methylation, but the results must be experimentally verified to confirm that the correct variations are identified. Pyrosequencing is a cost-effective technique highly suited for this purpose, providing quantitative analysis of short- to medium-length sequences with high accuracy. We now offer predesigned PyroMark CpG Assays for validating results from HumanMethylation450K and MethylationEPIC BeadChip experiments. These new predesigned assays for methylation array validation offer a convenient way to verify experimental results, and determine exact methylation levels of CpG sites of interest, without the need to manually design a separate assay for each individual target of interest. Our dedicated search page lets users simply enter the CpG loci identification numbers (CG#) of interest to easily search our assay database and order ready-to-use PyroMark CpG Assays.The advantages of GeneGlobe
QIAGEN"s GeneGlobe Web portal is a one-stop online interface that grants access to our extensive range of assay technologies in a gene- or pathway-specific context. GeneGlobe brings together state-of-the-art search and design algorithms, up-to-date sequence data, and our innovative technologies to facilitate discovery. Simply enter a search term for the gene or pathway of interest, and let GeneGlobe show you how to advance your research in epigenetics, or any other area of interest (see figure Ready-to-use methylation assays).Procedure
PyroMark CpG Assays include PCR and sequencing primers for quantification of DNA methylation by Pyrosequencing.A biotinylated PCR product is generated from the bisulfite converted DNA template and is bound to streptavidin-coated Sepharose beads. The beads are captured with the vacuum tool on the PyroMark Vacuum Workstation, where they are thoroughly washed and subsequently denatured, generating single-stranded DNA suitable for Pyrosequencing. This template DNA is released into the Pyrosequencing reaction plate containing the sequencing primer provided with PyroMark CpG Assays. After primer annealing, the plate is placed into the PyroMark instrument, and the sequencing reaction is started.
Applications
PyroMark CpG Assays enable highly accurate, quantitative methylation analysis of gene-specific human, mouse, and rat CpG sites as well as targets from the HumanMethylation450K and the MethylationEPIC BeadChip for epigenetics research.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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