Qiagen/AllTaq Master Mix and PCR Core Kits/50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2
市场价：
¥3280.00
美元价：
1968.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
AllTaq Master Mix and PCR Core Kits
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For ultrafast and versatile hot-start PCR in all applications
- Simple, visual pipetting control and gel tracking dyes monitor successful procedure
- Same 45-minute protocol for all targets including GC-rich and long targets up to 9 kb
- Versatile chemistry enables duplex PCR and the inclusion of internal controls
- 4x concentrated master mix and outstanding room temperature stability
- Guard-protected, hot-start chemistry provides superior specificity and sensitivity
The AllTaq Master Mix Kit and the AllTaq PCR Core Kit provide a convenient format for highly sensitive and specific hot-start PCR using any DNA or cDNA template. Both kits are highly suited for all PCR applications and provide the following features: visual pipetting controls, gel loading and tracking dyes, an ultrafast cycling protocol, extreme room temperature stability during and after reaction setup and a 4x-concentrated master mix format, allowing a higher sample input volume.
Want to try the AllTaq Master Mix Kit solution for the first time? Request a quote for a trial kit.
Want to try the AllTaq PCR Core Kit solution for the first time? Request a quote for a trial kit.
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- Product Details
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Cat No./ID:203123 AllTaq PCR Core Kit (250 U) $164.00 50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water |
Cat No./ID:203125 AllTaq PCR Core Kit (1000 U) $580.00 200 µl AllTaq Polymerase (5 U/µl), 2 x 1.2 ml AllTaq PCR Buffer (5x), 200 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 2 x50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 4 x 1.9 ml RNase-Free Water |
Cat No./ID:203127 AllTaq PCR Core Kit (5000 U) $2,600.00 5 x 200 µl AllTaq Polymerase (5 U/µl),9 x 1.2 ml AllTaq PCR Buffer (5x), 5 x 200 µl dNTP Mix (10 mM each), 4 x 200 µl Template Tracer (25x),9 x50 µl Master Mix Tracer (125x), 5 x 2 ml Q-Solution (5x), 2 x 1.2 ml MgCl2 (25 mM),16 x 1.9 ml RNase-Free Water |
Cat No./ID:203144 AllTaq Master Mix Kit (500) $376.00 For 500 x 20 µl PCR amplifications: 2 x 1.25 ml AllTaq Master Mix (4x), 1 x 200 µl Template Tracer (25x), 2 x 50 µl Master Mix Tracer (125x), 5 x 1.9 ml RNase-Free Water |
Cat No./ID:203146 AllTaq Master Mix Kit (2500) $1,603.00 For 2500 x 20 µl PCR amplifications: 10 x 1.25 ml AllTaq Master Mix (4x), 5 x 200 µl Template Tracer (25x),10 x 50 µl Master Mix Tracer (125x),20 x 1.9 ml RNase-Free Water |
AllTaq Master Mix and PCR Core Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The innovative formulation of the AllTaq Master Mix and AllTaq PCR Buffer facilitates the amplification of specific PCR products and delivers successful results at the first attempt, using the same protocol for all targets. During the annealing step, the buffer allows a high ratio of specific-to-nonspecific primer binding (see figure PCR buffer). The verified buffer composition is adapted to ultra-fast cycling conditions and simultaneously provides stringent primer-annealing conditions over a wide range of annealing temperatures. The AllTaq PCR Buffer also ensures perfect duplex capabilities. Optimization of PCR by varying the annealing temperature (see figureNo annealing temperature optimization) or the Mg2+ concentration is not required.The versatile chemistry enables amplification of long targets up to 9 kb as well as duplex PCR, providing flexible workflow planning using the same PCR kit. Q-solution provided in the AllTaq PCR Core Kit facilitates amplification of difficult secondary structures, such as GC-rich templates. The guard-protected, hot-start mechanism (see figure Principle of AllTaq hot-start mechanism) prevents premature PCR leakage, ensuring premium specificity and sensitivity down to a single target molecule (see figure Single copy detection).
Principle
The AllTaq Master Mix Kit provides a convenient, ready-to-use master mix formulation. The AllTaq PCR Core Kit offers AllTaq DNA Polymerase, AllTaq PCR buffer, dNTPs, MgCl2 and Q-solution in separate tubes, in case optimization of the PCR protocol is desired (see figure Amplification of long PCR fragments). Both kits provide orange Master Mix Tracer and blue Template Tracer in separate vials for optional use.At low temperatures, AllTaq DNA Polymerase is kept in an inactive state by an antibody and a novel guard molecule, which stabilize the complex (see figure Principle of AllTaq hot-start mechanism). This improves the stringency of the hot start and prevents any enzymatic activity at ambient temperature, allowing reaction setup without ice, facilitating automation. The enzyme is fully activated after the 2-minute incubation step at 95°C and starts amplifying with high specificity from the first cycle. The hot-start procedure eliminates extension from nonspecifically annealed primers and primer–dimers from the first cycle, ensuring highly specific and reproducible PCR. The innovative AllTaq PCR Master Mix and AllTaq Buffer facilitate ultra-fast cycling conditions and simultaneously provide stringent primer-annealing conditions over a wide range of annealing temperatures. They also ensure perfect duplex capabilities. Optimization of PCR by varying the annealing temperature or the Mg2+ concentration is not required.The blue and orange dyes in the PCR Template Tracer and in the PCR Master Mix Tracer, respectively, allow visual tracking of pipetted samples during the PCR setup, preventing errors. When the blue template is added to the orange PCR Master Mix, the color changes to green, confirming that sample was added (see figure Visual pipetting control). The use of these tracers is optional.Reactions can be directly loaded onto agarose gels after cycling. Each tracer dye allows monitoring of the loading process and efficient tracking of the subsequent electrophoresis (see figure Integrated tracking dyes).
Procedure
The AllTaq Master Mix Kit provides a convenient, ready-to-use 4x concentrated master mix formulation, which allows for a high template input volume to further boost sensitivity. The AllTaq PCR Core Kit offers AllTaq DNA Polymerase, PCR buffer, dNTPs, MgCl2 and Q-solution in separate tubes, if optimization of the PCR protocol should be desired. The orange Master Mix Tracer is optionally added to master mix or PCR buffer. When template dyed with the blue Template Tracer is added to the master mix, the color of the solution changes from orange to green, providing a visual indication of correct pipetting and reaction setup (see figurePCR procedure). The tracer dyes allow direct loading on agarose gels after PCR and efficient tracking of the subsequent electrophoresis. The dyes run at approximately 50 bp (orange) and 4000 bp (blue) on a 1% agarose gel (see figure Integrated tracking dyes). The versatile buffer system allows ultrafast PCR, in approximately 45 minutes, using the same protocol for any standard PCR and duplex PCR. A cycling protocol is provided for long templates, between 1–9 kb.The guard-protected, hot-start mechanism enables complete reaction setup at room temperature, eliminating the need for ice and allowing automated pipetting. After reaction setup, samples can be left at room temperature for more than 3 days before PCR cycling. Furthermore, the master mix is extremely stable, avoiding degradation during use and allowing storage at 2–8°C for up to 6 months.The AllTaq PCR Core Kit contains Q-Solution that can be added optionally. Q-solution facilitates amplification of difficult targets by modifying the melting behavior of nucleic acids (e.g., templates with a high degree of secondary structure or are GC rich).To further streamline PCR workflows, we recommend the QIAgility instrument for automated reaction setup and PCR analysis on the QIAxcel Advanced system.
Applications
The AllTaq Master Mix and AllTaq PCR Core Kits can be used for any PCR, for genotyping and genetic analysis or target detection from complex gDNA or cDNA templates, including reactions with multiple primer pairs or that include a wide range of amplicon sizes.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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