UCP Multiplex PCRKit
- Nucleic acid depleted – prevents background from residual DNA
- Robustnessagainst inhibitors and over a wide GC range ensures reliable profiling
- Sensitive multiplexing suited for genetic and microbiome/metagenome analysis
- Visual pipetting control and gel tracking dyes monitor successful procedure
- Room temperature reaction setup allows automated procedures
- Guard-protected hot-start chemistry provides superior specificity and sensitivity
The UCP Multiplex PCR Kit provides a convenient platform for highly-sensitive and specific multiplex hot-start PCR using any DNA or cDNA template.
Dedicated processes are implemented to enable Ultra-Clean Production of this master mix, including depletion of potential DNA background to a minimum. Combined with superior inhibitor resistance and minimized GC bias, this makes the kit an ideal choice for microbiome applications like 16S or 18S amplification. Additional features include: visual pipetting controls, a fast cycling protocol, room-temperature stability during and after reaction setup and a 4x concentrated master mix format, allowing for higher sample input volumes.
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Cat No./ID:206742 UCP Multiplex PCR Kit (100) $180.00 For 100 x 20 µl PCR reactions: 1 x 500 µl UCP Multiplex PCR Master Mix (4x), 1 x 200 µl UCP Template Tracer (25x), 1 x 50 µl UCP Master Mix Tracer (125x), 1 x 1.9 ml UCP Water |
Cat No./ID:206744 UCP Multiplex PCR Kit (500) $707.00 For 500 x 20 µl PCR reactions: 5 x 500 µl UCP Multiplex PCR Master Mix (4x), 2 x 200 µl UCP Template Tracer (25x), 1 x 50 µl UCP Master Mix Tracer (125x), 5 x 1.9 ml UCP Water |
Product Details
Ultra-Clean Production processes combined with nucleic acid depletion ensure that potential contamination with residual DNA from expression hosts, or other sources, is reduced to a minimum. All components are tested for bacterial and fungal DNA, using generic PCR Assays for 16S and 18S rRNA coding sequences. This facilitates accurate results in microbial testing, quality control procedures or microbiome/metagenome analysis and sequencing. Particularly, testing low biomass samples will benefit from ultra-clean reagents, but also improved inhibitor resistance and minimal GC-content bias ensure accurate alpha- or beta-diversity results. The high-multiplexing degree, long-read capability, plus integrated visual pipetting and gel loading dyes, further improve efficiency in genotyping and genetic testing workflows.
The UCP Multiplex Kit provides a convenient ready-to-use 4x concentrated master mix formulation, which allows a high-template input volume to further boost sensitivity.
The orange Master Mix Tracer is added optionally to the master mix or PCR buffer. When template dyed with the blue Template Tracer is added to the master mix, the color of the solution changes from orange to green, providing a visual indication of correct pipetting and reaction setup (see figure ""PCR procedure""). The Tracer dyes allow direct loading on agarose gels after PCR, and efficient tracking of the subsequent electrophoresis. The dyes run at approximately 50 bp (orange) and 4000 bp (blue) on a 1% agarose gel (see figure ""Easy visualization"").The versatile buffer system allows ultrafast PCR in approximately 90 minutes or less, providing an additional protocol for 16/18S sequence amplification with low complexity.The Guard-protected hot-start mechanism enables complete reaction setup at room temperature, preventing the use of ice and allowing automated pipetting.To further streamline PCR workflows, we recommend the QIAgility instrument for automated reaction setup, and PCR analysis on the QIAxcel system. For microbiome analysis, QIAGEN offers a range of dedicated products including DNeasy Power Soil Pro Kits for sample extraction, QIAseq 16S/ITS panels for next-generation sequencing or CLC Microbial Genomics Pro Suite software for bioinformatic analysis.The UCP Multiplex PCR Kit is ideally suited for procedures requiring a high purity and accuracy, like microbial testing, microbiome / metagenome analysis and sequencing, quality control, genotyping and genetic testing workflows.
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