QIAGEN PCR Cloning^plus Kit

The QIAGEN PCR Cloning^plus Kit combines the latest ligation technology with a unique combination of time-saving features for fast, easy, and highly efficient cloning of PCR products generated using Taq and other nonproofreading DNA polymerases. The QIAGEN PCR Cloning^plus Kit outperformed kits tested from other suppliers, ensuring successful results. Cloning into the pDrive Cloning Vector is much faster compared with TA-based cloning vectors (see figures "Highly specific cloning with a shorter ligation time of 30 min" and Highly specific cloning with a ligation time of 4 h", and table). The QIAGEN PCR Cloning^plus Kit is supplied with QIAGEN EZ Competent Cells, which do not require this time-consuming recovery incubation to achieve high-efficiency transformation (>10⁸ colony forming units (CFU) per microgram DNA; see figure "Transformation without recovery incubation").

The pDrive Cloning Vector (see figure "pDrive Cloning Vector") provides highly efficient cloning of PCR products through UA hybridization. The vector is supplied in a linear form with a U overhang at each 3" end, which hybridizes with high specificity to the A overhang of PCR products generated by Taq and other nonproofreading DNA polymerases. The pDrive Cloning Vector has a number of useful features designed to facilitate analysis of cloned PCR products. These include a large number of unique restriction enzyme recognition sites, universal sequencing primer sites, and promoters for in vitro transcription. In addition, the vector allows both ampicillin and kanamycin selection, as well as blue/white screening of recombinant colonies.

PCR products are efficiently cloned into the pDrive Cloning Vector in less time than is required for TA-based cloning vectors (see figures "Highly specific cloning with a shorter ligation time of 30 min" and Highly specific cloning with a ligation tme of 4 h"). Furthermore, as T is the most likely base to hybridize to noncomplementary bases (i.e., G, C, and T), vectors with a T overhang are more likely to self-anneal or to clone primers or annealed primers, leading to an increased number of false-positive colonies. In contrast, the higher cloning efficiency of the pDrive Cloning Vector indicates that U has a lower tolerance for nonspecific base pairing.

The Ligation Master Mix, supplied in a convenient premixed format, is specifically designed to provide optimal hybridization conditions for efficient cloning.

The use of QIAGEN EZ Competent Cells makes the cloning procedure even faster and more convenient. Transformed cells are usually incubated in SOC medium to recover and to have time to express antibiotic resistance. In contrast, QIAGEN EZ Competent Cells do not require this time-consuming recovery incubation to achieve high-efficiency transformation (>10⁸ colony forming units (CFU) per microgram DNA; see figure "Transformation without recovery incubation").

Components included with the QIAGEN PCR Cloning^plus Kit

Component	Included	Concentration
pDrive Cloning Vector		50 ng/µl
Ligation Master Mix		2x solution
Distilled Water		–
QIAGEN EZ Component Cells		–
SOC medium		1x solution

Simply mix the PCR product directly with pDrive Cloning Vector and Ligation Master Mix, incubate, and then add the ligation reaction to competent cells for transformation. QIAGEN EZ Competent Cells do not require a recovery incubation in SOC medium after transformation, and therefore can be plated immediately onto agar/ampicillin plates.

The QIAGEN PCR Cloning^plus Kit procedure (see flowchart "PCR Cloning Kit procedure") is much faster than topoisomerase-mediated, TA-based, and conventional sticky- and blunt-end cloning methods. Ligation takes 30 minutes and transformation and plating using QIAGEN EZ Competent Cells takes only 10 minutes, making the complete procedure — from PCR product to plated cells — just 40 minutes.