Qiagen/REPLI-g UltraFast Mini Kit/DNA Polymerase, Buffers, and Reagents for 25 x 20 μl ultrafast whole genome amplification reactions/150033
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¥5240.00
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3144.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
REPLI-g UltraFast Mini Kit
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For ultrafast highly uniform whole genome amplification from small or precious samples
- Fast 1–1.5 hour whole genome amplification
- Obtain unlimited DNA for downstream applications
- Reproducible amplification from a variety of starting materials
- Highly stable DNA suitable for long-term storage
A lack of sufficient quantities of genomic DNA for genomic analysis can be overcome by global amplification of all DNA within a sample (whole genome amplification). The REPLI-g UltraFast Kit includes REPLI-g UltraFast DNA Polymerase, which enables highly uniform and accurate whole genome amplification from small samples in just 60–90 minutes, resulting in typical DNA yields of 7 μg per 20 μl reaction. A variety of starting materials can be used, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue, and cells. The DNA generated is suitable for direct use in a range of genetic analyses.
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Cat No./ID:150033 REPLI-g UltraFast Mini Kit (25) $262.00 DNA Polymerase, Buffers, and Reagents for 25 x 20 μl ultrafast whole genome amplification reactions |
Cat No./ID:150035 REPLI-g UltraFast Mini Kit (100) $776.00 DNA Polymerase, Buffers, and Reagents for 100 x 20 μl ultrafast whole genome amplification reactions |
The REPLI-g UltraFast Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The average DNA yield is7 µg per 20 µl reaction after 90 minutes with standard quality samples. If time is very limited, sufficient product is available for downstream genetic analysis after just 45 minutes (see figure "Consistent and high yields of amplified DNA"). Negative control samples (without template DNA) are not amplified, providing a reliable indicator of the presence/absence of sample or contaminating DNA (see figure "Consistent and high yields of amplified DNA").
Researcher feedback
"In the past two years, we have been using the Repli-g Ultra Fast Mini Kit extensively for multiple projects. The multiple displacement amplification performed with the Repli-g Kit was able to faithfully amplify all DNA products at very low error rate and high coverage. The downstream applications we haveused the kit forinclude most often NGS, but also qPCRand microarray. Moreover, we are working on implementing this technology in microfluidic or lab-on-a-chip systems, since the method requires no sophisticated thermal cycling program." Dr. Rong Fan, Assistant Professor of Biomedical Engineering, Yale University, US.Principle
The availability of sufficient quantities of genomic DNA for genomic analysis is often lacking. Whole genome amplification overcomes this limitation by global amplification of all DNA within a sample, providing sufficient quantities to perform all analyses on the same DNA sample. REPLI-g UltraFast technology provides fast and highly uniform DNA amplification across the entire genome, with minimum sequence bias. The method is based on multiple displacement amplification (MDA) technology — isothermal genome amplification utilizing a new, uniquely processive DNA polymerase that gives DNA products of up to 100 kb in length in a much shorter time, while still avoiding the high sequence bias of PCR-based amplification methods. In addition, a unique alkaline denaturation buffer guarantees gentle denaturation of genomic DNA and avoids fragmentation of template DNA caused by heat denaturation methods. This ensures more sensitive and more reliable results in downstream assays.
Procedure
Amplification of genomic DNA using the REPLI-g UltraFast Mini Kit involves 3 basic steps (see figure "Purified genomic DNA procedure"). First, the sample (10 ng purified genomic DNA, 0.5 µl whole blood, or 300 tissue culture cells) undergoes gentle alkaline denaturation, avoiding fragmentation and damage of template DNA. Next the sample is neutralized, and finally incubated with REPLI-g UltraFast DNA Polymerase at 30°C. The amplified DNA is ready to use after 60 minutes without further purification.
Applications
REPLI-g amplified genomic DNA can be used in a variety of downstream applications, including:
- Genotyping (e.g., SNP, STR, microarray)
- End-point PCR, quantitative real-time PCR
- Sequencing
- Comparative genome hybridization
Specifications
Features | Specifications |
| Amplification | Whole genomic DNA |
| Applications | Genotyping, microarray, PCR, real-time PCR |
| Denaturation step | Alkaline |
| Maximum input volume | 10 ng DNA, 0.5 µl whole blood, ~300 cells/µl |
| Minimal pipetting volume needed | 1 µl |
| Quality assessment | No |
| Reaction time | 60–90 minutes |
| Reaction volume | 20 µl |
| Samples per run (throughput) | Mid |
| Starting amount of DNA | >10 ng purified genomic DNA |
| Starting material | Genomic DNA, blood, cells |
| Technology | Multiple Displacement Amplification (MDA) |
| Yield | ~10 µg |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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