
RT2 SYBR® Green qPCR Mastermixes
- Designed exclusively for use with RT2 and EpiTect Methyl II qPCR Assays
- Delivers specific amplification with minimal primer-dimers
- Available with ROX, fluorescein, or without dye
- For usewith qPCR instruments under standard cycling conditions
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Cat No./ID:330500 RT² SYBR Green qPCR Mastermix (2) $210.00 Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Arrays or 200 x 25 µl reactionsor 400 x 10µl reactions |
Cat No./ID:330501 RT² SYBR Green qPCR Mastermix (8) $779.00 Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactionsor 2000 x 10µlreactions |
Cat No./ID:330502 RT² SYBR Green qPCR Mastermix (12) $1,156.00 Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactionsor 3000 x 10 µl reactions |
Cat No./ID:330503 RT² SYBR Green qPCR Mastermix (24) $1,684.00 Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactionsor 6000 x 10 µl reactions |
Cat No./ID:330504 RT² SYBR Green qPCR Mastermix (6) $612.00 Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Arrays or 600 x 25 µl reactionsor 1500 x 10µl reactions |
Cat No./ID:330509 RT² SYBR Green qPCR Mastermix (25 ml) $1,585.00 Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactionsor 5000 x 10 µl reactions |
Cat No./ID:330510 RT² SYBR Green Fluor qPCR Mastermix (2) $210.00 Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactionsor 400 x 10 µl reactions |
Cat No./ID:330511 RT² SYBR Green Fluor qPCR Mastermix (8) $767.00 Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactionsor 2000 x 10 µl reactions |
Cat No./ID:330512 RT² SYBR Green Fluor qPCR Mastermix (12) $1,128.00 Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactionsor 3000 x 10 µl reactions |
Cat No./ID:330513 RT² SYBR Green Fluor qPCR Mastermix (24) $1,709.00 Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactionsor 6000 x10µl reactions |
Cat No./ID:330514 RT² SYBR Green Fluor qPCR Mastermix (6) $599.00 Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactionsor 1500 x 10 µl reactions |
Cat No./ID:330519 RT² SYBR Green Fluor qPCR Mastermix (25ml) $1,577.00 Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactionsor 5000 x 10 µl reactions |
Cat No./ID:330520 RT² SYBR Green ROX qPCR Mastermix (2) $207.00 Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactionsor 400 x 10µl reactions |
Cat No./ID:330521 RT² SYBR Green ROX qPCR Mastermix (8) $792.00 Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactionsor 2000 x 10µlreactions |
Cat No./ID:330522 RT² SYBR Green ROX qPCR Mastermix (12) $1,128.00 Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactionsor 3000 x 10 µl reactions |
Cat No./ID:330523 RT² SYBR Green ROX qPCR Mastermix (24) $1,692.00 Contains 24 x 1.35 mltubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactionsor 6000 x 10 µl reactions |
Cat No./ID:330524 RT² SYBR Green ROX qPCR Mastermix (6) $618.00 Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactionsor 1500 x 10 µl reactions |
Cat No./ID:330529 RT² SYBR Green ROX qPCR Mastermix (25ml) $1,585.00 Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactionsor 5000 x 10µl reactions |
Product Details
The high-performance real-time PCR enabled by RT² SYBR Green qPCR Mastermixesdemonstrateshigh amplification efficiencies (see figure "High amplification efficiency over a wide dynamic range") andhigh levels of sensitivity and specificity (see figure"Tighter control of polymerase activity yields greater specificity").
RT2 SYBR Green qPCR Mastermixes contain real-time PCR buffer, a high-performance, HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. Some mastermixes contain either fluorescein or ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate SYBR Green results by preventing the amplification of primer–dimers and other nonspecific products.
Mastermixes without ROX or fluorescein
RT2 SYBR Green qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation not requiring a reference dye, including Bio-Rad models CFX96, CFX384; Bio-Rad/MJ Research Chromo4, DNA Engine Opticon, DNA Engine Opticon 2; Roche LightCycler 480 (96-well and 384-well); Eppendorf Mastercycler ep realplex without ROX filter set; and Cepheid SmartCycler.
Mastermixes with fluorescein
RT2 SYBR Green Fluor qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses fluorescein as a reference dye, including the Bio-Rad models iCycler, iQ5, MyiQ, and MyiQ2.
Mastermixes with ROX
RT2 SYBR Green ROX qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses ROX as a reference dye, including QIAGEN’s Rotor-Gene Q; Applied Biosystems models 5700, 7000, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast 96-well block, 384-well block), StepOnePlus, ViiA 7 (Standard and Fast 96-well block, 384-well block); Eppendorf Mastercycler ep realplex with or without ROX filter set; Stratagene models Mx3000P, Mx3005P, Mx4000; and Takara TP-800.
Product Resources
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