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当前位置: 首页 > 产品中心 > peptide > Qiagen/RT2SYBR®绿色qPCR母料/包含2 x 1.35 ml试管:用于2 x 96孔RT²;PCR阵列,1 x 384孔RT²;PCR阵列或200 x 25µ;l反应;或400 x 10&微型的;l反应/330500
商品详细Qiagen/RT2SYBR®绿色qPCR母料/包含2 x 1.35 ml试管:用于2 x 96孔RT²;PCR阵列,1 x 384孔RT²;PCR阵列或200 x 25µ;l反应;或400 x 10&微型的;l反应/330500
Qiagen/RT2SYBR®绿色qPCR母料/包含2 x 1.35 ml试管:用于2 x 96孔RT²;PCR阵列,1 x 384孔RT²;PCR阵列或200 x 25µ;l反应;或400 x 10&微型的;l反应/330500
Qiagen/RT2SYBR®绿色qPCR母料/包含2 x 1.35 ml试管:用于2 x 96孔RT²;PCR阵列,1 x 384孔RT²;PCR阵列或200 x 25µ;l反应;或400 x 10&微型的;l反应/330500
商品编号: 330500
品牌: Qiagen
市场价: ¥4200.00
美元价: 2520.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

RT2 SYBR® Green qPCR Mastermixes

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For use with RT2 Profiler,RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays
  • Designed exclusively for use with RT2 and EpiTect Methyl II qPCR Assays
  • Delivers specific amplification with minimal primer-dimers
  • Available with ROX, fluorescein, or without dye
  • For usewith qPCR instruments under standard cycling conditions
RT2 SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, andare required for guaranteed qPCR performance of theRT2 qPCR Primer Assays,RT2 Profiler PCR Arrays,RT2 lncRNA qPCR Assays, andRT2 lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT2 SYBR Green qPCR Mastermixesare also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.
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Cat No./ID:330500
RT² SYBR Green qPCR Mastermix (2)
$210.00
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Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Arrays or 200 x 25 µl reactionsor 400 x 10µl reactions
Cat No./ID:330501
RT² SYBR Green qPCR Mastermix (8)
$779.00
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Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactionsor 2000 x 10µlreactions
Cat No./ID:330502
RT² SYBR Green qPCR Mastermix (12)
$1,156.00
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Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactionsor 3000 x 10 µl reactions
Cat No./ID:330503
RT² SYBR Green qPCR Mastermix (24)
$1,684.00
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Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactionsor 6000 x 10 µl reactions
Cat No./ID:330504
RT² SYBR Green qPCR Mastermix (6)
$612.00
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Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Arrays or 600 x 25 µl reactionsor 1500 x 10µl reactions
Cat No./ID:330509
RT² SYBR Green qPCR Mastermix (25 ml)
$1,585.00
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Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactionsor 5000 x 10 µl reactions
Cat No./ID:330510
RT² SYBR Green Fluor qPCR Mastermix (2)
$210.00
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Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactionsor 400 x 10 µl reactions
Cat No./ID:330511
RT² SYBR Green Fluor qPCR Mastermix (8)
$767.00
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Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactionsor 2000 x 10 µl reactions
Cat No./ID:330512
RT² SYBR Green Fluor qPCR Mastermix (12)
$1,128.00
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Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactionsor 3000 x 10 µl reactions
Cat No./ID:330513
RT² SYBR Green Fluor qPCR Mastermix (24)
$1,709.00
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Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactionsor 6000 x10µl reactions
Cat No./ID:330514
RT² SYBR Green Fluor qPCR Mastermix (6)
$599.00
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Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactionsor 1500 x 10 µl reactions
Cat No./ID:330519
RT² SYBR Green Fluor qPCR Mastermix (25ml)
$1,577.00
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Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactionsor 5000 x 10 µl reactions
Cat No./ID:330520
RT² SYBR Green ROX qPCR Mastermix (2)
$207.00
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Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactionsor 400 x 10µl reactions
Cat No./ID:330521
RT² SYBR Green ROX qPCR Mastermix (8)
$792.00
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Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactionsor 2000 x 10µlreactions
Cat No./ID:330522
RT² SYBR Green ROX qPCR Mastermix (12)
$1,128.00
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Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactionsor 3000 x 10 µl reactions
Cat No./ID:330523
RT² SYBR Green ROX qPCR Mastermix (24)
$1,692.00
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Contains 24 x 1.35 mltubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactionsor 6000 x 10 µl reactions
Cat No./ID:330524
RT² SYBR Green ROX qPCR Mastermix (6)
$618.00
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Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactionsor 1500 x 10 µl reactions
Cat No./ID:330529
RT² SYBR Green ROX qPCR Mastermix (25ml)
$1,585.00
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Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactionsor 5000 x 10µl reactions
RT2 SYBR® Green qPCR Mastermixes are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

9
Tighter control of polymerase activity yields greater specificity.
9
High amplification efficiency over a wide dynamic range.
Performance
RT² SYBR Green qPCR Mastermixes contain all of the optimized reagents and buffers needed for SYBR Green based real-time PCR with RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCRAssays, and RT2 lncRNA PCR Arrayson all real-time PCR instruments.

The high-performance real-time PCR enabled by RT² SYBR Green qPCR Mastermixesdemonstrateshigh amplification efficiencies (see figure "High amplification efficiency over a wide dynamic range") andhigh levels of sensitivity and specificity (see figure"Tighter control of polymerase activity yields greater specificity").

Principle

RT2 SYBR Green qPCR Mastermixes contain real-time PCR buffer, a high-performance, HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. Some mastermixes contain either fluorescein or ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate SYBR Green results by preventing the amplification of primer–dimers and other nonspecific products.

Mastermixes without ROX or fluorescein

RT2 SYBR Green qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation not requiring a reference dye, including Bio-Rad models CFX96, CFX384; Bio-Rad/MJ Research Chromo4, DNA Engine Opticon, DNA Engine Opticon 2; Roche LightCycler 480 (96-well and 384-well); Eppendorf  Mastercycler  ep realplex without ROX filter set; and Cepheid SmartCycler.

Mastermixes with fluorescein

RT2 SYBR Green Fluor qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses fluorescein as a reference dye, including the Bio-Rad models iCycler, iQ5, MyiQ, and MyiQ2.

Mastermixes with ROX

RT2 SYBR Green ROX qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses ROX as a reference dye, including QIAGEN’s Rotor-Gene Q; Applied Biosystems models 5700, 7000, 7300, 7500 (Standard and Fast), 7700, 7900HT  (Standard and Fast 96-well block, 384-well block), StepOnePlus, ViiA 7 (Standard and Fast 96-well block, 384-well block); Eppendorf Mastercycler ep realplex with or without ROX filter set; Stratagene models Mx3000P, Mx3005P, Mx4000; and Takara TP-800.

Applications
RT2 SYBR Green qPCR Mastermixes are suitable for use in real-time PCR applications, especially RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays.

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FAQs (16)
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Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
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How does HotStart PCR help minimize nonspecific amplification events?
FAQ ID -2676
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What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?
FAQ ID -2678
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How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?
FAQ ID -2679
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Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
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What is the delta Rn value?
FAQ ID -2681
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What is the threshold cycle or Ct value?
FAQ ID -2682
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Why are my qPCR Ct values too low (< 12)="" in="" my="" qrt-pcr="">
FAQ ID -2684
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Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
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How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?
FAQ ID -2688
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Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
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What do I need to complete a RT² qPCR Primer Assay?
FAQ ID -2707
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Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
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Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
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What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products?
FAQ ID -2715
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Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
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Instrument Technical Documents (1)
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(EN) - RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis
Show details
Kit Handbooks (1)
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RT2 Profiler PCR Array Handbook
For pathway-focused gene expression profiling using real-time RT-PCR
Show details
Application Notes (1)
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(EN) - Purification of total RNA from peripheral blood mononuclear cells
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Safety Data Sheets (18)
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MSDS RT² SYBR Green Fluor qPCR Mastermix (12)
MSDS RT² SYBR Green Fluor qPCR Mastermix (6)
MSDS RT² SYBR Green Fluor qPCR Mastermix (2)
MSDS RT² SYBR Green Fluor qPCR Mastermix (24)
MSDS RT² SYBR Green Fluor qPCR Mastermix (25ml)
MSDS RT² SYBR Green Fluor qPCR Mastermix (8)
MSDS RT² SYBR Green qPCR Mastermix (12)
MSDS RT² SYBR Green qPCR Mastermix (2)
MSDS RT² SYBR Green qPCR Mastermix (24)
MSDS RT² SYBR Green qPCR Mastermix (25 ml)
MSDS RT² SYBR Green qPCR Mastermix (6)
MSDS RT² SYBR Green qPCR Mastermix (8)
MSDS RT² SYBR Green ROX qPCR Mastermix (12)
MSDS RT² SYBR Green ROX qPCR Mastermix (2)
MSDS RT² SYBR Green ROX qPCR Mastermix (24)
MSDS RT² SYBR Green ROX qPCR Mastermix (25ml)
MSDS RT² SYBR Green ROX qPCR Mastermix (8)
MSDS RT² SYBR Green ROX qPCR Mastermix (6)
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。