Qiagen/miRCURY LNA miRNA PCR Assays/339306
市场价：
¥2400.00
美元价：
1440.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
miRCURY LNA miRNA PCR Assays
Print
For extremely sensitive and specific miRNA quantification using LNA-optimized, SYBR® Green-based miRNA PCR
- Unmatched sensitivity allows miRNA quantification from just 1 pg total RNA
- Great for samples with low RNA yield, such as serum/plasma, FFPE sections and LCM samples
- High specificity discriminates closely related miRNAs and mature miRNA from precursors
- Fast and simple two-step protocol takes less than 3 hours
- Full miRBase coverage enables miRNA profiling from any organism
miRCURY LNA miRNA PCR Assays are individual miRNA PCR primer sets that enable extremely sensitive and specific miRNA quantification with the miRCURY LNA miRNA PCR System. Both forward and reverse PCR amplification primers are miRNA-specific and are optimized with LNA technology. Assays are available in tube or plate format with 200 reactions per tube or well.
Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!
- Buy Products
- Product Details
Buy Products
Cat No./ID:339306 miRCURY LNA miRNA PCR Assay $120.00 Contains forward and reverse primers for 200 reactions; for SYBR® Green-based detection |
miRCURY LNA miRNA PCR Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Unmatched sensitivity
The exceptional sensitivity of miRCURY LNA miRNA PCR Assays is achieved by combininguniversal reverse transcription with LNA-enhanced and Tm-normalized primers. This combination enables accurate and reliable quantification of individual miRNAs from as little as 1 pg of total RNA input in the initial first-strand cDNA synthesis (see figure Accurate quantitation from down to 1 pg total RNA starting material).Compared with other miRNA real-time PCR systems that use either stem-loop or standard DNA primers, the LNA-enhanced primers offer significantly increased sensitivity, especially for AT-rich miRNAs (see figure LNA-enhanced primers result in greatly increased sensitivity compared to DNA primers).The low sample requirements also enable miRNA quantitation using total RNA purified from difficult samples such as LCM samples and serum/plasma (see figuresDetection of differential expression of miRNAs in LCM specimens from FFPE tissue sections and Differences in miRNA expression between serum samples).Fully validated and optimized
All wet-lab validated miRCURY LNA miRNA PCR Assays have been optimized to be as sensitive as possible. Over 80% of assays detect at least 5 miRNA copies in the PCR reaction (see figure Serial dilution of hsa-miR-181a). The primer sets have also been validated for specific amplification of the target and for minimal background signal (see figure Specific and sensitive amplification of miRNA).The only platform with perfect specificity
The miRCURY LNA miRNA PCR Assays are the only miRNA quantification platform with absolute specificity (see the miRQC study published in Nature Methods) and out-performed another probe-based miRNA qPCR platform in specificity tests (see figure Specificity test with probe-based miRNA qPCR system). Perfect specificity eliminates false positives and ensures only robust andreliable miRNA signals.Superior discrimination
The incorporation of LNA in both the forward and reverse PCR amplification primersmakes it possible to design assays that can distinguish between miRNA sequences that differ by only one nucleotide (see figure Single-nucleotide discrimination). In addition, the assays can discriminate between mature miRNA sequences and precursor miRNA.Fast, easy and reproducible
The easy-to-follow, 3-hour protocol saves you bothtime and effort in the laboratory. By using the same RT reaction as the template in all subsequent PCR reactions, the procedure is greatly simplified compared with systems that require miRNA-specific first-strand synthesis. The number of pipetting steps is reduced to a minimum, and technical variation is minimized. This makes it possible to achieve extremely high reproducibility from day-to-day and even site-to-site.miRCURY LNA miRNA PCR Assays have been optimized for use with the miRCURY LNA RT Kit and the miRCURY LNA SYBR Green PCR Kit. Use of other reagents will affect the quality of the results.Principle
A unique system for miRNA profiling
miRCURY LNA miRNA PCR Assays offer the best combination of performance and ease-of-use available on the microRNA real-time PCR market by combining universal RT with LNA PCR amplification (see figure Schematic outline of the miRCURY LNA miRNA PCR System).Universal RT makes it possible to use one first-strand cDNA synthesis reactionas thetemplate for multiple miRNA real-time PCR assays. This saves precious samples, reduces technical variation and saves time in the laboratory.Plus, both the forward and reverse PCR amplification primers are miRNA specific and optimized with LNA. This provides 1) exceptional sensitivity and extremely low background, enabling accurate quantitation of very low miRNA levels and 2) highly specific assays that allow discrimination between closely related miRNA sequences.Coverage
Over 20,000 assays are available covering all organisms in miRBase 20. Over 1,400 assays are fully wet-lab validated for sensitivity, specificity, efficiency and background on both synthetic as well as different biological samples. The remaining assays are in silico-validated using a comprehensive design algorithm that ensures high-quality, species-specific, LNA-enhanced assays with optimal sensitivity and specificity within each organism. This means that several different assays may target the same sequence. Ultimately, the assay for each species is selected based on the genetic background of the organism. If you are working with novel miRNAs,such asfrom an NGS experiment, custom-designed LNA miRNA primer sets for any miRNA are also available.Reference gene assays for normalization
Nine reference gene primer sets are available, enabling high-quality data normalization and generation of reliable data from many different sample types (see table below). These control primer sets target endogenous, small non-coding RNAs that are constitutively and relatively stably expressed across different human tissues (see figure Expression levels of reference genes in different human tissues). The control reference genes cover a large range of Cq values, offering the possibility to accurately and reliably normalize across a range of miRNA expression levels.The control primer sets have been validated as reference genes for the miRCURY LNAmiRNA PCR System and work optimally with the miRCURY LNA RT Kit and the miRCURY LNA SYBR Green PCR Kits.It is very important to always confirm the consistent and unvarying expression of a gene in all tissues and under all treatments of an experiment before choosing it as the reference. We recommend that you test a number of reference genes and use software such as GeNorm and NormFinder (part of the GenEx software) to choose and validate reference genes.| Product name | Target organism | Alternative names | NCBI reference |
|---|---|---|---|
| Control primer set, SNORD38B (hsa) | hsa | U38B; RNU38B | NR_001457 |
| Control primer set, SNORD44 (hsa) | hsa | U44; RNU44 | NR_002750 |
| Control primer set, SNORD48 (hsa) | hsa | U48; RNU48 | NR_002745 |
| Control primer set, SNORD49A (hsa) | hsa | U49; U49A; RNU49 | NR_002744 |
| Control primer set, SNORA66 (hsa) | hsa | U66; RNU66 | NR_002444 |
| Control primer set, 5S rRNA (hsa) | hsa, mmu | V00589 | |
| Control primer set, U6 snRNA (hsa, mmu) | hsa, mmu, rno | U6 | X59362 |
| Control primer set, RNU5G snRNA (mmu, hsa) | mmu, hsa, rno | Rnu5a; U5a; Rnu5g | NR_002852 |
| Control primer set, RNU1A1 (mmu, hsa) | mmu, hsa, rno | Rnu1a-1; Rnu1a1 | NR_004411 |
| Control primer set, SNORD65 (mmu) | mmu | U65; RNU65 | NR_028541 |
| Control primer set, SNORD68 (mmu) | mmu | U68; RNU68 | NR_028128 |
| Control primer set, SNORD110 (mmu) | mmu | U110; RNU110 | NR_028547 |
Procedure
The miRCURY LNA miRNA PCR Assay system is an miRNA-specific, LNA-based system designed for sensitive and accurate detection of miRNA by quantitative real-time PCR using SYBR Green. The first step of the procedure is universal reversetranscription, followed by real-time PCR amplification with LNA-enhanced primers(see figure miRCURY LNA miRNA PCR System at a glance).
Applications
miRCURY LNA miRNA PCR Assays are used as part of the miRCURY LNA miRNA PCR System for:
- Mature miRNA quantification
- snoRNA detection
Product Resources
Product citations in scientific articles
Powered by Bioz
See more details on BiozCustomers who bought these products also bought
- Cat No./ID:339340
miRCURY LNA RT Kit
For 8–64 cDNA synthesis reactions: 5x RT SYBR Green Reaction Buffer, 5x RT Probe Reaction Buffer, 10x RT Enzyme Mix, UniSp6, RNA Spike-in template, RNase-Free Water
$373.00Add To Cart - Cat No./ID:339346
miRCURY LNA SYBR Green PCR Kit (600)
For 600 reactions: 2X miRCURY SYBR Green Master Mix, RNase-Free Water$406.00Add To Cart - Cat No./ID:339390
RNA Spike-In Kit, For RT
Contains the UniSp2, UniSp4, and UniSp5 RNA Spike-in Template Mix and the cel-miR-39-3p RNA Spike-in Template$165.00Add To Cart - Cat No./ID:339345
miRCURY LNA SYBR Green PCR Kit (200)
For 200 reactions: 2X miRCURY SYBR Green Master Mix, RNase-Free Water$180.00Add To Cart - Cat No./ID:339317
miRCURY LNA miRNA Custom PCR Assay
Contains forward and reverse primers for 200 reactions; for SYBR® Green-based detection
Select Targets - Cat No./ID:339320
miRCURY LNA miRNA PCR Starter Kit
Two miRCURY LNA PCR Assays of your choice, spike-in control Assay (UniSp6), one candidate endogenous control assay (miR-103-3p) and all reagents for 20 reverse transcription reactions and 100 PCR amplifications; for SYBR® Green-based qPCR detection
Select Targets - Cat No./ID:339347
miRCURY LNA SYBR Green PCR Kit (4000)
For 4000 reactions: 2X miRCURY SYBR Green Master Mix, RNase-Free Water$1,506.00Add To Cart - Cat No./ID:217204
miRNeasy Serum/Plasma Advanced Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-Free Reagents and Buffers$461.00Add To Cart - Cat No./ID:330701
RT2 lncRNA qPCR Assay
For 200 reactions; mix of 2 gene-specific primers provided in solution (200 µl), with 10 µM of each primerSelect Targets - Cat No./ID:218300
miScript Primer Assay (100)
10x miScript Primer Assay (contains one miRNA-specific primer)Select Targets - Cat No./ID:217184
miRNeasy Serum/Plasma Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers$489.00Add To Cart - Cat No./ID:331551
QIAseq miRNA Library QC qPCR Assay Kit
Includes 52 RNA Spike-ins, 10x RT Enzyme Mix, 5x Reaction Buffer, 10x RT Primer Mix, UniSP6 RNA Spike-in template and qPCR assays for 103a-3p, 191-5p, UniSp6, 451a, 23a-3p, 30c-5p, UniSp-100 and UniSp-101$529.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
