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商品详细Qiagen/RT2qPCR引物分析/330001
Qiagen/RT2qPCR引物分析/330001
Qiagen/RT2qPCR引物分析/330001
商品编号: 330001
品牌: Qiagen
市场价: ¥3000.00
美元价: 1800.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

RT2 qPCR Primer Assays

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For accurate and reliable gene expression analysis using laboratory-verified assays

  • Complete genome coverage for multiple species
  • Amplifies a single amplicon with uniform PCR efficiency
  • Less than 5 minutes hands-on time

RT² qPCR Primer Assays are specifically designed and experimentally verified for real-time PCR analysis. The rigorous assay verification criteria ensure PCR specificity and efficiency for reliable and accurate gene expression analysis results.

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Cat No./ID:330001
RT2 qPCR Primer Assay (200)
$150.00
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RT2 qPCR Primer Assay
RT2 qPCR Primer Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Principle

RT² qPCR Primer Assays use SYBR® Green-based quantitative real-time PCR technology to provide a sensitive and reliable tool for gene expression analysis.

Each assay utilizes a proprietary and experimentally verified algorithm for the design of gene-specific qPCR primers with uniform PCR efficiency and amplification conditions. Each lot of every assay is further wet-bench tested for real-time PCR performance for specificity and amplification efficiency. Amplification of a single product of the correct size with high PCR efficiency (>90%) is guaranteed when the assays are used with RT² SYBR® Green qPCR Mastermixes. The uniform PCR efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses.

Applications
RT2 qPCR Primer Assays are highly suited for applications including validation of microarray-derived gene expression data, confirming gene expression knockdown by RNAi, identifying and confirming disease-associated biomarkers, and monitoring phenotypic changes related to gene expression. Multiple RT2 qPCR Primer Assays can also be used to examine a focused panel of genes.

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Performance Data (7)
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PCR_Array_4x96_384-Well_Conversion Spreadsheet
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RT2 Profiler Housekeeping Genes PCR Array Data Analysis 1808
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RT2 Profiler PCR Array 384HT Data Analysis Spreadsheet 1808
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RT2 Profiler PCR Array Data Analysis Spreadsheet 1904
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RT2 Profiler RNA QC PCR Array Data Analysis Spreadsheet 1808
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RT2 qPCR Assay Data Analysis 1808 Spreadsheet
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Universal Custom PCR Array Conversion
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Brochures & Guides (1)
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Total RNA Discovery - (EN)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
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FAQs (30)
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How important is the RNA purification process, for obtaining reliable qRT-PCR results?
FAQ ID -2655
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Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
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What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?
FAQ ID -2678
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How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?
FAQ ID -2679
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Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
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What is the delta Rn value?
FAQ ID -2681
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What is the threshold cycle or Ct value?
FAQ ID -2682
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Why are my qPCR Ct values too low (< 12)="" in="" my="" qrt-pcr="">
FAQ ID -2684
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Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
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Why is my no template control (NTC) real-time Ct value < 35="" cycles="" in="" my="" qpcr="">
FAQ ID -2686
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How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?
FAQ ID -2688
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Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
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Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
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What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?
FAQ ID -2691
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What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?
FAQ ID -2692
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What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?
FAQ ID -2693
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How do I determine the amplification efficiency of my qPCR assay?
FAQ ID -2694
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Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
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How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?
FAQ ID -2696
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Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
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What do I need to complete a RT² qPCR Primer Assay?
FAQ ID -2707
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What RT² qPCR Primer Assays are available?
FAQ ID -2708
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Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased?
FAQ ID -2709
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Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
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How does QIAGEN control the quality of the RT² qPCR Primer Assays?
FAQ ID -2711
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What does the RT² qPCR Primer Assay product information mean when it says that it recognizes another transcript of the same gene?
FAQ ID -2712
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Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
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Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
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What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products?
FAQ ID -2715
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What is the recommended amount of input template for each RT² qPCR Primer Assay?
FAQ ID -2716
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Kit Handbooks (1)
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RT2 qPCR Primer Assay Handbook

For gene expression analysis by real-time RT‑PCR

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Instrument Technical Documents (1)
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(EN) - RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis
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Scientific Posters (1)
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Explore the RNA Universe!
Poster for download
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Supplementary Protocols (10)
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ABI 7500 & ABI 7500 FAST (Software Version 2.0.4) instrument setup instructions for RT2 Profiler PCR Arrays
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ABI 7900HT (for SDS Software 2.1, 2.3 and 2.4) instrument setup instructions for RT2 Profiler PCR Arrays
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ABI StepOnePlus (for Software Version 2.0) instrument setup instructions for RT2 Profiler PCR Arrays
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Bio-Rad CFX96 and CFX384 instrument setup instructions for RT2 Profiler PCR Arrays
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Bio-Rad iCycler & iQ Real-Time PCR Systems (for Software Version 3.1) instrument setup instructions for RT2 Profiler PCR Arrays
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Eppendorf Mastercycler ep realplex instrument setup instructions for RT2 Profiler PCR Arrays
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Stratagene Mx3000P qPCR System real-time PCR run setup instructions for RT2 Profiler PCR Arrays
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Rotor-Gene Q real-time PCR run setup instructions for RT2 Profiler PCR Arrays
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Roche LightCycler 480 real-time PCR run setup instructions for RT2 Profiler PCR Arrays
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Life Technologies ViiA7 (ViiA 7 Software v1.2) instrument setup instructions for RT2 Profiler PCR Arrays
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White Papers (1)
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A systematic guideline for developing the best real-time PCR primers
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Safety Data Sheets (1)
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MSDS RT2 qPCR Primer Assay (200)
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。