miScript Primer Assays
For quantification of mature miRNA using the miScript PCR System
- Sensitive and specific detection and quantification of miRNA
- Custom assay design for mature miRNAs not listed in miRBase
- Convenient ordering via GeneGlobe
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Cat No./ID:218300 miScript Primer Assay (100) $98.80 10x miScript Primer Assay (contains one miRNA-specific primer) |
Product Details
The miScript PCR System enables sensitive, specific miRNA quantification and profiling using SYBR® Green-based real-time PCR. The miScript PCR System covers all the steps involved in conversion of RNA to cDNA and subsequent real-time PCR detection of miRNAs.
For mature miRNA detection and quantification using the miScript PCR System, the following components are used:
miScript Primer Assay — miScript Primer Assays are miRNA-specific forward primers designed to detect mature miRNAs using the most up-to-date information from miRBase.
For detection of mature miRNA, cDNA prepared in a reverse transcription reaction using miScript HiSpec Buffer or miScript HiFlex Buffer from the miScript II RT Kit serves as the template for real-time PCR. Real-time PCR is performed using an miRNA-specific miScript Primer Assay (forward primer) and the miScript SYBR Green PCR Kit, which contains the miScript Universal Primer (reverse primer) and QuantiTect SYBR Green PCR Master Mix.
For detection of other noncoding RNA, cDNA prepared in a reverse transcription reaction using miScript HiFlex Buffer from the miScript II RT Kit serves as the template for real-time PCR. Real-time PCR is performed using a miScript Primer Assay (forward primer) and the miScript SYBR Green PCR Kit, which contains the miScript Universal Primer (reverse primer) and QuantiTect SYBR Green PCR Master Mix. If a miScript Primer Assay for the small RNA of interest is not already available, one can be custom-designed.
miScript Primer Assays are used as part of the miScript PCR System for:
- Mature miRNA quantification
- snoRNA detection
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