mericon Pathogen Detection Assays
For real-time PCR detection of pathogen DNA in food or animal feed
- Highly sensitive and specific detection of a broad range of pathogens
- Single, streamlined detection protocol for all targets
- Dedicated mericon bacterial DNA isolation kits available
- PCR run time of 73 minutes on the QIAGEN Rotor-Gene Q
- Full license for PCR without additional costs
mericon Pathogen Detection Assays are a suite of kits for the target-specific detection of bacterial pathogen DNA in food, animal feed, and pharmaceutical products. All of the assays use the same real-time PCR-based protocol. They are part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication and GMO DNA detection. mericon assays perform optimally on the Rotor-Gene Q, but have also been validated for block thermal cyclers.
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Cat No./ID:290013 mericon Salmonella spp Kit (24) $209.00 For 24 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290015 mericon Salmonella spp Kit (96) $752.00 For 96 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290025 mericon L. monocytogenes Kit (96) $752.00 For 96 reactions: PCR Assay L. monocytogenes, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290035 mericon Campylobacter spp Kit (96) $1,017.00 For 96 reactions: PCR Assay Campylobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290055 mericon VTEC stx 1/2 Kit (96) $1,102.00 For 96 reactions: PCR Assay VTEC stx 1/2, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290065 mericon Cronobacter spp Kit (96) $960.00 For 96 reactions: PCR Assay Cronobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290073 mericon S. aureus Kit (24) $329.00 For 24 reactions: PCR Assay S. aureus, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290085 mericon Quant Legionella spp Kit $1,202.00 For 96 reactions: PCR Assay Quant Legionella spp, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290095 mericon QuantL. pneumophila Kit (96) $1,222.00 For 96 reactions: PCR Assay Quant L. pneumophila, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290103 mericon Shigella spp Kit (24) $323.00 For 24 reactions: PCR Assay Shigella spp,Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290113 mericon Y. enterocolitica Kit (24) $326.00 For 24 reactions: PCR Assay Y. enterocolitica, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290123 mericon Listeria spp Kit (24) $207.00 For 24 reactions: PCR Assay Listeria spp,Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution |
Cat No./ID:290233 mericon E. coli STEC O-Type Kit (24) $246.00 For 24 reactions: PCR Assay E. coli O157 STEC O-Type, Internal Control, Multiplex PCR Master Mix, RNase-Free Water |
Cat No./ID:290405 mericon E. coli O157 Screen Plus Kit (96) Inquire For96 reactions: PCR Assay E. coli O157 Screen Plus, Internal Control, Multiplex PCR Master Mix, RNase-Free Water |
Product Details
mericon Campylobacter spp Kit
The mericon Campylobacter spp Kit is for the specific detection of several Campylobacter subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Campylobacter DNA in a reaction and it exhibits high specificity for pathogens this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter spp Kit cross-reactivity study")..
mericon Campylobacter triple Kit
The mericon Campylobacter triple Kit is designed for the detection of Campylobacter jejuni, C. coli, and C. laridis in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of C. jejuni, C. coli, or C. laridis DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter triple Kit cross-reactivity study").
mericon Cronobacter spp Kit
The mericon Cronobacter spp Kit is for the specific detection of Chronobacter subspecies in enrichment cultures of food and animal feed samples. Due to a recent change in taxonomy and according to regulation of the EU Commission (365/2010) the name Cronobacter spp replaces Enterobacter sakazakii. The assay can detect as few as 10 copies of Cronobacter DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Cronobacter spp Kit cross-reactivity study").
mericon Quant Legionella spp Kit
The mericon Quant Legionella spp Kit is for the specific detection and quantification of several Legionella species in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of Legionella DNA in a reaction and it exhibits high specificity for pathogens of that genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant Legionella spp Kit cross-reactivity study").
mericon Quant L. pneumophila Kit
The mericon Quant L. pneumophila Kit is for the specific detection and quantification of Legionella pneumophila in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of L. pneumophila DNA in a reaction and it exhibits high specificity for L. pneumophila . In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant L. pneumophila Kit cross-reactivity study").
mericon Listeria spp Kit
The mericon Listeria spp Kit is for the specific detection of several Listeria subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Listeria DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Listeria Kit cross-reactivity study").
mericon L. monocytogenes Kit
The mericon L. monocytogenes Kit is for the specific detection of Listeria monocytogenes in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of L. monocytogenes DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens, even closely-related Listeria species (see the table "Results of mericon L. monocytogenes Kit cross-reactivity study").
mericon Salmonella spp Kit
The mericon Salmonella spp Kit is for the specific detection of several Salmonella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Salmonella DNA in a reaction and itexhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Salmonella spp Kit cross-reactivity study").
mericon Shigella spp Kit
The mericon Shigella spp Kit is for the specific detection of several Shigella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Shigella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Shigella spp Kit cross-reactivity study").
mericon S. aureus Kit
The mericon S. aureus Kit is for the specific detection of Staphylococcus aureus in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of S. aureus DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon S. aureus Kit cross-reactivity study").
mericon VTEC stx1/2 Kit
The mericon VTEC stx1/2 Kit is for the specific detection of the strains of Escherichia coli that produce verotoxins, also called Shiga toxins, in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of verotoxin-producing E. coli DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon VTEC stx1/2 Kit cross-reactivity study").
mericon Y. enterocolitica Kit
The mericon Y. enterocolitica Kit is designed for the detection of Yersinia enterocolitica in enrichment cultures of food and animal feed samples. The kit specifically detects human pathogen serotypes 0:3, 0:5, 0:6, 0:8, 0:9, and 0:5:27. The assay can detect as few as 10 copies of Yersinia enterocolitica DNA in a reaction and exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Y. enterocolitica Kit cross-reactivity study").
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Campylobacter jejuni | + | Campylobacter coli | + |
| Campylobacter laridis | + | Campylobacter upsaliensis | + |
| Salmonella spp. | – | VTEC stx1 | – |
| Clostridium perfringens | – | VTEC stx2 | – |
| Legionella erythra | – | Yersinia enterocolitica | – |
| Staphylococcus aureus | – | Listeria monocytogenes | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Campylobacter jejuni | + | Campylobacter coli | + |
| Campylobacter laridis | + | Campylobacter upsaliensis | – |
| Salmonella spp. | – | VTEC stx1 | – |
| Clostridium perfringens | – | VTEC stx2 | – |
| Legionella pneumophila | – | Yersinia enterocolitica | – |
| Staphylococcus aureus | – | Listeria monocytogenes | – |
| Escherichia coli | – | Bacillus cereus | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Cronobacter sakazakii | + | Listeria monocytogenes | – |
| Staphylococcus aureus | – | Escherichia coli | – |
| Salmonella spp. | – | Legionella erythra | – |
| Clostridium perfringens | – | Bacillus cereus | – |
| Legionella pneumophila | – | Yersinia enterocolitica | – |
| Shigella flexneri | – | VTEC stx1 | – |
| Campylobacter jejuni | – | VTEC stx2 | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Legionella pneumophila | + | Legionella erythra | + |
| Salmonella thyphimurium | – | Escherichia coli | – |
| Shigella flexneri | – | Bacillus cereus | – |
| Clostridium perfringens | – | Yersinia enterocolitica | – |
| Campylobacter jejuni | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Legionella pneumophila | + | Legionella erythra | – |
| Salmonella thyphimurium | – | Escherichia coli | – |
| Shigella flexneri | – | Bacillus cereus | – |
| Clostridium perfringens | – | Yersinia enterocolitica | – |
| Campylobacter jejuni | – |
td>–
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Listeria monocytogenes | + | Cronobacter sakazakii | – |
| Listeria welshimeri | + | Legionella pneumophila | – |
| Listeria seeligeri | + | Staphylococcus aureus | – |
| Listeria ivanovii | + | Bacillus cereus | – |
| Listeria innocua | + | Yersinia enterocolitica | – |
| Listeria marthii | + | VTEC | – |
| Listeria grayi | + | Legionella erythra | – |
| Listeria rocoutiae | + | Shigella flexneri | – |
| Campylobacter jejuni | – | Clostridium perfringens | – |
| Escherichia coli | – |
th>Resulttd>Legionella erythra
| Pathogen | Result | Pathogen | |
|---|---|---|---|
| Listeria monocytogenes | + | Cronobacter sakazakii | – |
| Clostridium perfringens | – | Bacillus cereus | – |
| Staphylococcus aureus | – | Legionella erythra | – |
| Salmonella spp. | – | Campylobacter jejuni | – |
| Yersinia enterocolitica | – | Legionella pneumophila | – |
| Shigella flexneri | – | VTEC stx1 | – |
| Escherichia coli | – | VTEC stx2 | – |
| Listeria innocua | – | Listeria welshimeri | – |
| Listeria ivanovii spp. Ivanovii | – | Listeria grayi | – |
| Listeria seeligeri | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Salmonella spp | + | Cronobacter sakazakii | – |
| Clostridium perfringens | – | Bacillus cereus | – |
| Staphylococcus aureus | – | Legionella erythra | – |
| Listeria monocytogenes | – | Campylobacter jejuni | – |
| Yersinia enterocolitica | – | Legionella pneumophila | – |
| Shigella flexneri | – | VTEC stx1 | – |
| Escherichia coli | – | VTEC stx2 | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Shigella spp. | + | Listeria monocytogenes | – |
| Staphylococcus aureus | – | Escherichia coli | – |
| Salmonella spp. | – | Bacillus cereus | – |
| Clostridium perfringens | – | Yersinia enterocolitica | – |
| Campylobacter jejuni | – | Legionella erythra | – |
| VTEC stx1 | – | VTEC stx2 | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Staphylococcus aureus | + | Listeria monocytogenes | – |
| Cronobacter sakazakii | – | Escherichia coli | – |
| Salmonella spp. | – | Legionella erythra | – |
| Clostridium perfringens | – | Bacillus cereus | – |
| Legionella pneumophila | – | Yersinia enterocolitica | – |
| Shigella flexneri | – | VTEC stx1 | – |
| Campylobacter jejuni | – | VTEC stx2 | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| VTEC stx1 | + | VTEC stx2 | + |
| Campylobacter jejuni | – | Escherichia coli | – |
| Salmonella spp. | – | Legionella erythra | – |
| Clostridium perfringens | – | Bacillus cereus | – |
| Legionella pneumophila | – | Yersinia enterocolitica | – |
| Staphylococcus aureus | – | Listeria monocytogenes | – |
| Pathogen | Result | Pathogen | Result |
|---|---|---|---|
| Yersinia enterocolitica | + | Listeria monocytogenes | – |
| non-pathogenic Yersiniaenterocolitica | – | Escherichia coli | – |
| Staphylococcus aureus | – | Bacillus cereus | – |
| Clostridium perfringens | – | Salmonella spp. | – |
| Campylobacter jejuni | – | Legionella erythra | – |
| VTEC stx1 | – | VTEC stx2 | – |
Detection of pathogens using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.
mericon PCR Assays include an internal control to control potential inhibiton. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").
The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.
| Internal control amplified | Tested DNA amplified | Meaning |
|---|---|---|
| + | + | Sample is positive for the target DNA |
| + | – | Sample is negative for the target DNA |
| – | – | PCR failed due to inhibition |
DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit is for the isolation of bacterial DNA from Gram-negative bacteria. The mericon Bacteria Plus Kit is for the isolation of bacterial DNA from Gram-positive bacteria.
The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN"s Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.
The results are displayed as easy-to-interpret PCR curves.
mericon Pathogen Detection Assays are for real-time PCR-based detection of specific bacterial pathogens in food, animal feed, and pharmaceutical products.
Specifications
Features | Specifications |
| Quantitative/qualitative | Qualitative (Quantitative for Quant L pneumophilia and Quant Legionella spp) |
| Recommended sample prep | mericon DNA Bacteria Kit (Gram-negative bacteria) or mericon DNA Bacteria Plus Kit (Gram-positive bacteria) or QIAamp UCP Pathogen Mini Kit (Legionella-specific) |
| RUO/CE/ASR | RUO |
| Sample type | Bacterial preculture from food and water samples after concentration, e.g. filtration (for Legionella- specific Kits) |
| Thermal cycler | Rotor-Gene Q and other cyclers |
| What detected | DNA of respective pathogen |
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