Qiagen/miRCURY LNA miRNA定制PCR面板/339330
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商品介绍
miRCURY LNA miRNA Custom PCR Panels
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Custom 96- and 384-well plates of LNA-enhanced, SYBR® Green-based miRNA qPCR assays
- Combine predesigned verified and custom-designed LNA PCR Assays in single-use, ready-to-use plates
- Customize for your favored plate type, layout, PCR instrument and batch size
- Great for sensitive, specific and reproducible miRNA profiling of a medium to large number of samples
- Use our new easy-to-use online design tool
miRCURY LNA miRNA Custom Panels are ready-to-use 96- and 384-well plates containing individual miRCURY LNA miRNA PCR Assays according to your particular requirements. Both forward and reverse PCR amplification primers are miRNA-specific and are optimized with LNA technology, enabling extremely sensitive and specific miRNA quantification with the miRCURY LNA miRNA PCR System. Each well contains sufficient primer set for one 10 µl reaction.
Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!
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Cat No./ID:339330 miRCURY LNA Custom PCR Panel (8) Go to GeneGlobe8 identical, ready-to-use 96- or 384-well plates; each well contains primers sufficient for one 10 µl reaction; for SYBR® Green-based detection |
Cat No./ID:339332 miRCURY LNA Custom PCR Panel Additional Plates (4) Go to GeneGlobeAdditional miRCURY LNA Custom PCR Panel plates; set of 4 plates; only available in addition to the base plates ordered through the core product (cat. no. 339330) |
miRCURY LNA miRNA Custom PCR Panels are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Top performance in specificity
The miRCURY LNA miRNA PCR System is the only miRNA quantification platform with absolute specificity (see the miRQC study published in Nature Methods) and out-performed another probe-based miRNA qPCR platform in specificity tests (see figure Specificity test alongside competitor probe-based miRNA qPCR system). This eliminates false positives and ensures only robust and reliable miRNA signals. The easy-to-follow, 3-hour protocol saves you both time and effort in the laboratory. Simply dilute the synthesized cDNA, combine it with the miRCURY LNA SYBR Green Master Mix and aliquot into the PCR plate, which is then ready to run in your real-time PCR instrument. The number of pipetting steps is reduced to a minimum, reducing technical variation. As a result, you can achieve extremely high reproducibility from day-to-day and even site-to-site (see figuresExcellent day-to-day reproducibility and Excellent site-to-site correlation). High reproducibility is even possible when profiling difficult samples, such as RNA purified from serum (see figure Excellent reproducibility between RT reactions on total RNA from serum).Principle
Design your own 96- or 384-well miRNA qPCR plates based on a fully flexible layout and several convenient, predefined layouts. Place predesigned miRCURY LNA miRNA PCR Assays or your own Custom miRCURY PCR Assays into the panel using an intuitive and easy-to-use online plate configurator. The panels are delivered ready-to-use, with one single assay per well for a 10 µl reaction volume. Just add cDNA and miRCURY LNA SYBR Green Master Mix.
Coverage
Custom miRNA PCR Panels are available in 2 different formats:- 96-well, ready-to-use: design your panels from one of three available layouts. Choose from our vast selection of experimentally validated LNA miRNA PCR primer sets and reference gene primer sets for human, mouse and rat miRNA quantification.
- 384-well, ready-to-use: design your panels from one of four available layouts. Choose from our vast selection of experimentally validated LNA miRNA PCR primer sets and reference gene primer sets for human, mouse and rat miRNA quantification.
Add custom assays
It is now possible to add custom assays to your custom plate. Custom assays are designed through one of our custom assay design tools. Design LNA-enhanced PCR primers for sequences not available among our predesigned assays, e.g., for novel miRNAs identified using next-generation sequencing.Procedure
miRNA expression profiling with miRCURY LNA miRNA PCR Panels is straightforward and robust. First, prepare cDNA using the miRCURY LNA RT Kit. Second, add a premix of cDNA, miRCURY LNA SYBR Green PCR Master Mix and RNase-free water to a miRCURY miRNA PCR Panel. Third, run the reaction in a real-time PCR cycler.
Applications
miRCURY LNA Custom PCR Panels are used as part of the miRCURY LNA miRNA PCR System for:
- Pathway- and disease-focused miRNA profiling
- Biomarker discovery
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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