Qiagen/miRCURY LNA miRNA miRNome PCR Panels/339322
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商品介绍
miRCURY LNA miRNA miRNome PCR Panels
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For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBR® Green-based miRNA qPCR
- Unmatched sensitivity enables profiling 752 miRNAs using only 40 ng total RNA
- Available for human, mouse and rat miRNAs
- Fast and easy to use with no pre-amplification required
- Reliable discrimination of closely related miRNAs and mature and precursor miRNAs
miRCURY LNA miRNA miRNome PCR Panels enable exceptionally sensitive and specific miRNA expression profiling using LNA technology and the miRCURY LNA miRNA PCR System. These predesigned PCR primer sets are pre-aliquoted in 384-well PCR plates and are ready for use. Available for human, mouse and rat miRNAs, the miRCURY LNA miRNA miRNome PCR Panels allow you to profile up to 752 human or rodent miRNAs using only 40 ng total RNA, with no required pre-amplification.
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Cat No./ID:339322 miRCURY LNA miRNA miRNome PCR Panels Go to GeneGlobemiRCURY LNA miRNA PCR Panels for PCR-based miRNome profiling, available in 384-well format; for SYBR® Green-based detection |
miRCURY LNA miRNA miRNome PCR Panels are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Top performance in specificity
The miRCURY LNA miRNA PCR platform is the only miRNA quantification platform with absolute specificity (see the miRQC study published in Nature Methods) and out-performed another probe-based miRNA qPCR platform in specificity tests (see figure Specificity test alongside competitor probe-based miRNA qPCR system). This eliminates false positives and ensures only robust and reliable miRNA signals.Fast andreproducible results from minimal sample amounts
The miRCURY LNA miRNA miRNome PCR Panels enable profiling of 752 miRNAs from just 40 ng of total RNA, so you canperform high-quality miRNA expression profiling in samples that contain very little total RNA, such as FFPE sections and serum/plasma (see figuresExpression profiling of 730 miRNAs using 40 ng total RNA from tumor and normal FFPE sections and Expression profiling of 368 miRNAs using total RNA from 35 μl serum). The easy-to-follow, 3-hour protocol saves you both time and effort in the laboratory. Simply dilute the synthesized cDNA, combine itwith the miRCURY LNA SYBR Green Master Mix and aliquot into the 384-well PCR plate, which is then ready to run in your real-time PCR instrument. The number of pipetting steps is reduced to a minimum, reducing technical variation. As a result, you can achieve extremely high reproducibility from day-to-day and even site-to-site (see figuresExcellent day-to-day reproducibility and Excellent site-to-site correlation). High reproducibility is even possible when profiling difficult samples, such as RNA purified from serum (see figure Excellent reproducibility between RT reactions on total RNA from serum).Principle
Features
The miRCURY LNA miRNA miRNome PCR Panelsenable exceptionally sensitive, high-throughput expression profiling from minimal amounts of starting material. The combination of a universal cDNA synthesis reaction and ready-to-use PCR panels provides fast and easy miRNA profiling. The panels are provided in 384-well plates thatcontain dried-down primers for one reaction per welland simply require addition of cDNA and miRCURY LNA SYBR Green Master Mix prior to real-time PCR amplification.TheLNA-enhanced, miRNA-specific PCR primers offer exceptional sensitivity and extremely low background, enabling accurate quantitation of very low miRNA levels. Plus, high specificity allows discrimination between closely related miRNA sequences, with a quick and easy protocol that gives you results in just 3 hours.Coverage
Two panels covering 752 human miRNAs and two panels covering 752 mouse and rat miRNAs are available. Panel I for human and mouse/rat contains high-priority miRNA primer sets. These miRNAs are generally more highly expressed, more likely to be differentially expressed in disease or more often cited in the literature. Panel II contains primer sets for the most important miRNAs not available on Panel I. To view the list of primer sets in each panel, download the plate layout files.The miRNome PCR Panels also include 6 potential reference gene primer sets –three small RNA reference genes (U6, SNORD38B and SNORD49A for human and U6 snRNA, RNU5G, RNU1A for mouse and rat) and three miRNA reference genes (miR-103-3p, miR-191-5p and hsa-miR-423-5p or mmu-miR-423-3p) – all found on Panel I.Panel I also contains qPCR assays for the 5 synthetic RNAs in the RNA Spike-in Kit (cel-miR-39-3p, UniSp2, UniSp4, UniSp5 and UniSp6) that may be used for monitoring the cDNA synthesis or RNA purification. Finally, each panel contains an inter-plate calibrator in triplicate and an empty negative control.Version update
The updated miRCURY LNA miRNA miRNome PCR Panels include 54 updated assays on Panel I and 33 updated assays on Panel II. The assays target the same miRNAs as v3 panels. In addition, the two spike-in primer sets UniSp5 and UniSp6 have been updated for improved performance. Their respective target templates will be the same.Procedure
The easy-to-follow, 3-hour protocol saves you both time and effort in the laboratory. Simply dilute the synthesized cDNA, combine it with the miRCURY LNA SYBR Green master mix and aliquot into the 384-well PCR plate containing the pre-aliquoted miRCURY LNA miRNA miRNome Panels. The plate is nowready to run in your real-time PCR instrument.
Applications
miRNA quantification in serum, plasma, exosomes, urine and other liquid biopsies; biomarker discovery
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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