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商品详细Qiagen/miScript miRNA模拟/219600

Qiagen/miScript miRNA模拟/219600

商品编号： 219600

品牌： Qiagen

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

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产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

qPCR Assays & Instruments
CNV qPCR Assays & Arrays3
Food Safety Testing26
Microbial DNA qPCR Assays & Panels8
miRNA qPCR Assay & Panels28
mRNA/IncRNA qPCR Assays & Panels31
Somatic Mutations qPCR Assays & Panels3

miScript miRNA Mimics

For studies on miRNA function and gene regulation using synthetic miRNA

Ready-to-transfect mimics behave like endogenous miRNAs
Available for all human, mouse, and rat miRNAs in miRBase
Highly flexible formats, scales, and grades
Custom design available for miRNAs not listed in miRBase
Easy to order via GeneGlobe

miScript miRNA Mimics are chemically synthesized, double-stranded RNAs which mimic mature endogenous miRNAs after transfection into cells. miScript miRNA Mimics are available at cell-culture grade (>90% purity) or animal grade (HPLC purified; for in vivo applications). For flexible screening, miScript miRNA Mimic Plates enable researchers to order any predesigned or custom-designed miScript miRNA Mimics in their choice of layout in 96-well or 384-well plates. miScript miRNA Inhibitors are suitable for similar experiments. For transfection, QIAGEN provides HiPerFect Transfection Reagent for miRNA Mimic transfection and Attractene Transfection Reagent for miRNA Mimic-DNA cotransfection.

Please beware that miScript miRNA Mimics will be discontinued on July 1, 2019. Products will still be available for reordering, but the product webpage will be removed. We strongly recommend miRCURY LNA miRNA Mimics which have superior performance.

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Cat No./ID:219600

miScript miRNA Mimic

Go to GeneGlobe

1 nmol (cell-culture grade), 5 nmol (cell-culture grade), or 20 nmol (animal grade) miScript miRNA Mimic, provided in tubes

miScript miRNA Mimics are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

miScript miRNA Mimic stable for 72 hours.

HeLa S3 cells (6 x 10⁴ cells/well) were transfected with a negative control or an miR-1 Mimic using HiPerFect Transfection Reagent.Twenty-four hours prior to measurement, cells were transfected with a luciferase reporter construct carrying an miR-1 binding site using Attractene Transfection Reagent. After the time points indicated, miR-1 levels were detected by measuring luciferase activity.

Downregulation at protein level after miR-1 mimic transfection.

HDAC4 is predicted to be a target of miR-1 and has 2 miR-1 binding sites in the 3" UTR (Chen, J.F. et al. [2006] The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nat. Genet. 38, 228.). HeLa S3 cells (8 x 10⁴ cells/well) were transfected using HiPerFect Transfection Reagent anda range of concentrations ofmiR-1 Mimic. miR-1 #1, miR-1 #2, and miR-1 #3 are different batches of miR-1 Mimic.After 24 and 48 hours, [A] HDAC4 protein level was analyzed by western blotting. Tubulin was also analyzed as an internal control (not shown).No effect was observed after 24 hours.After 48 hours, protein level was significantly decreased. [B] HDAC4 transcript level was analyzed by real-time PCR using the miScript System after transfection using 50 nM miR-1 Mimic. The negative control was AllStars Negative Control. Results were normalized to GAPDH and to the negative control and are presented as 2^-ΔΔCT. Transcript levels did not change significantly at either the 24-hour or 48-hour time point.

Comparable downregulation by endogenous miRNA and miScript miRNA Mimic.

miR-1 is not endogenously expressed in HeLa S3 cells. miR-16 is endogenously expressed at high levels in HeLa S3 cells (Lagos-Quintana, M. et al. [2001] Identification of novel genes coding for small expressed RNAs. Science 294, 853.). Using HiPerFect Transfection Reagent, HeLa S3 cells (6 x 10⁴ cells/well) were transfected with a luciferase reporter construct with a binding site for miR-1 in the 3" UTR; cotransfected with a luciferase reporter construct with a binding site for miR-1 in the 3" UTR and with a miR-1 Mimic; or transfected with a luciferase reporter construct with a binding site for miR-16 in the 3" UTR. Twenty-four hours later, luciferase activity was measured. miR-1 Mimic resulted in a similar decrease in luciferase level as endogenous miR-16.

Performance

miScript miRNA Mimics cause a decrease in gene expression similar to that observed with endogenous miRNAs (see figure "Comparable downregulation by endogenous miRNA and miScript miRNA Mimic"). The observed decrease in gene expression is often mediated at the translational level (see figure "Downregulation at protein level after miR-1 Mimic transfection"). miScript miRNA Mimics efficiently inhibit gene expression for up to 72 hours after transfection (see figure "miScript miRNA Mimic stable for 72 hours").

Principle

Transfection of mimics followed by downstream gene expression analysis or phenotypic analysis, is performed to elucidate the targets and roles of particular miRNAs. These experiments enable study of the biological effects of misregulation of individual miRNAs as well as confirmation of specific genes as targets of individual miRNAs. Reduced gene expression after transfection of mimic provides evidence that the miRNA under study is involved in regulation of that gene. miScript miRNA Inhibitors can also be used for similar studies. Typically, such experiments involve transfection of mimic or inhibitor, or alternatively cotransfection with a vector construct which carries the miRNA-binding site fused to a reporter gene. Downstream analysis can include reporter assays, real-time PCR, microarray analysis, or protein analysis.

Procedure

miScript miRNA Mimics are available for every human, mouse, rat, and virus miRNA in the current version of miRBase, the microRNA database. QIAGEN"s GeneGlobe database is continually updated to ensure that mimic and inhibitor designs match the most up-to-date version of miRBase. This ensures that you are always working with the most accurate and current miRNA sequence information possible. If the miRNA of interest is not available in miRBase, a mimic for your human, mouse, rat, or virus miRNA can be custom synthesized. Simply visit the custom miScript miRNA product design page, enter a name for the mimic, provide the target sequence, and our design tool will provide a custom mimic ready to order.

Flexible formats enable either low-throughput analysis of small numbers of mimics or high-throughput screening of hundreds of mimics. miScript miRNA Mimics are available in tubes (1 nmol or 5 nmol) at cell-culture-grade (>90% purity provided by proprietary synthesis and purification processes). For in vivo applications, HPLC-purified, animal-grade miScript miRNA Mimics can be ordered at the 20 nmol scale (provided in tubes). For medium- to high-throughput applications, 96-well plates (1 nmol or 5 nmol), or 384-well plates (1 nmol) are available to suit your experimental requirements. Simply upload an Excel sheet of the predesigned (listed in miRBase) or custom (not listed in miRBase) miScript miRNA Mimics in your preferred plate layout, and then view and order the plate.

Applications

miRNA research
Gene regulation research

Specifications

Features	Specifications
Design	Predesigned and custom design avaliable
Format	Single tubes, 96-well & 384 well plates
Modification	None
Scale	1 nmol, 5 nmol, 20 nmol
Sequence provided	No
Species	Human, mouse, rat

Product Resources

Application Notes (1)

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	(EN) - Serum miRNA and lncRNA detection as a potential biomarker of lung cancer	Show details

Brochures & Guides (3)

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	microRNA Sample and Assay Technologies - (EN)	Show details
	Total RNA Discovery - (EN) Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow	Show details
	microRNA Sample and Assay Technologies (eBook)	View

FAQs (5)

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	Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors? FAQ ID -1984	View
	What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays? FAQ ID -1985	View
	What kind of molecules are miScript miRNA Mimics? FAQ ID -1986	View
	What is the "seed sequence" of a miRNA? FAQ ID -2262	View
	Where can I find QIAGEN products for a specific gene or gene product? FAQ ID -803	View

Kit Handbooks (2)

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	(EN) - Guidelines for experiments using miScript Target Protectors For miRNA function research	Show details
	(EN) Guidelines for miRNA mimic and miRNA inhibitor experiments	Show details

Articles (1)

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	Analysis of the role of let-7 miRNAs in cell proliferation using miScript miRNA Mimics and Cignal Reporter Assays	View

Safety Data Sheets (1)

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	MSDS miScript miRNA Mimic

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品牌介绍

QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。

公司介绍

品牌分类

发现与翻译研究人类识别与取证 Diagnostics & Clinical Research 仪器与自动化

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服务热线：4000-520-616

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QQ ：1570468124

手机：18915418616

官网：http://www.qiagen.com/

miScript miRNA Mimics

Buy Products

Product Details

Specifications

Product Resources

Customers who bought these products also bought

AllStars Negative Control siRNA (5 nmol)

miScript Inhibitor Neg. Control (5 nmol)

miScript Primer Assay (100)

HiPerFect Transfection Reagent (0.5 ml)

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AllStars Neg. Control siRNA (20 nmol)

miScript SYBR Green PCR Kit (200)