Qiagen/Microbial DNA qPCR Arrays/330261
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商品介绍
Microbial DNA qPCR Arrays
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For real-time PCR-based, application-specific microbial identification or profiling
- Profile microbial species, virulence genes, or antibiotic resistance genes
- Simple procedure for a variety of sample types
- Integrated controls ensure reliability of results
Microbial DNA qPCR Arrays are collections of assays to identify and profile microbial species, virulence factorgenes or antibiotic resistance genes from a variety of sample types. Species identification assays target bacterial 16S rRNA genes and fungal ribosomal RNA genes, and each array includes controls for host DNA, presence of bacterial DNA and success of the PCR reaction. Microbial qPCR Mastermixis also included in the array kit.The simplicity of the product format and operating procedure allow routine, reliable identification or profiling of microbial species and genes from samples in any research laboratory with access to a real-time PCR instrument.What is the difference between microbial identification and profiling? Identification is determining the microbe’s presence or absence in your sample which requires running a No Template Control during your analysis. Profiling is determining the microbe’s relative expression in two or more experimental conditions for which you will need to run a reference sample and a normalizer (provided by QIAGEN).
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Cat No./ID:330261 Microbial DNA qPCR Arrays Go to GeneGlobeArray plate and master mix for detection of microbial species or genes |
Microbial DNA qPCR Arrays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Linearity and dynamic range
Microbial DNA qPCR Assays display linear amplification across a range from 10 to 106 copies of DNAtemplate (see figure, Linearity and sensitivity of Microbial DNA qPCR Assays).Lower limit of quantification (LLOQ)
The LLOQ is the lowest concentration of template that still falls into the linear range of the standard curve (see figure, Limit of detection versus lower limit of quantification). Across all Microbial DNA qPCR Assays, 93% have an LLOQ of <100 gene copies (see figure, The LLOQ for all Microbial DNA qPCR Assays reveals high sensitivity). 92% of microbial identification assays meet this LLOQ, as do 95% of virulence factor gene assays and 97% of antibiotic resistance gene assays (see figures, The LLOQ for microbial identification Microbial DNA qPCR Assaysreveals high sensitivity,The LLOQ for virulence factorgene detection Microbial DNA qPCR Assaysreveals high sensitivity, andThe LLOQ for antibiotic resistance gene detection Microbial DNA qPCR Assays reveals high sensitivity).Specificity
Each Microbial DNA qPCR Assay is stringently tested to ensure that it detects only one target species or gene (see figure, Microbial DNA qPCR Assays are highly specific). For assays that do detect more than one target, a list of detected targets and in silico predictions is included on the product sheet.This specificity is maintained even when samples have high species complexity, such as in stool, sputum, and plaque (see figure,Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples), and is verifiable by sequencing methods (see figure,Specificity of the Antibiotic Resistance Genes Microbial DNA qPCR Array is confirmed by pyrosequencing).Reproducibility
Microbial DNA qPCR Assays are highly reproducible, both in intra- and inter-individual variability tests (see figure, Microbial DNA qPCR Arrays generate reliably reproducible results).Principle
Microbial DNA qPCR Assays are designed to detect bacterial 16S rRNA gene and fungal ribosomal rRNA gene sequences for species identification, as well as detecting virulence factor genes and antibiotic resistance genes using PCR amplification primers and hydrolysis-probe detection.Microbial DNA qPCR Arrays are 96- or 384-well plates comprising related assays; for example, microbial species associated with respiratory infections, or microbes that are studied for biodefense. Controls are integrated on each plate to test for the presence of fungal DNA, bacterial DNA, host genomic DNA, and success of the PCR reaction, ensuring confidence in the results.
Procedure
The Microbial DNA qPCR Array procedure is simple and can be carried out in any laboratory with a real-time PCR instrument. DNA is isolated using the QIAamp kit that is suited for the sample type, and then mixed with the appropriate Microbial qPCR Mastermix. The mixture is aliquotted across the array plate, and real-time PCR is performed to obtain the raw CT values for each assay. The complimentary data analysis software is then used to profile genes or species in the sample.
Applications
Microbial DNA qPCR Arrays are highly suited for identification or profiling of microbial species or genes in a variety of sample types.For example, the Vaginal Flora Microbial DNA qPCR Array can be used to explore the underlying causes of bacterial vaginosis (see figures,The Vaginal Flora Microbial DNA qPCR Array provides accurate profiling for cervical swab samples andVaginal samples positive for Gardnerella vaginalis also show changes in commensal and bacterial vaginosis-related microbes compared to healthy samples), while the Antibiotic Resistance Genes Microbial DNA qPCR Array canidentify these genes insamples from the gut or from sewage (see figures,The Microbial DNA qPCR Array screens gut microbiota for the presence of antibiotic resistance genes and The Antibiotic Resistance Genes Microbial DNA qPCR Array identified antibiotic resistance genes in sewage samples).
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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