mericon E. coli O157 Detection Kits
- Screen for E. coli serotype O157 and virulence genes with Screen Plus Kit
- Detect O157:H7 and differentiate from other STEC-containing E. coli strains with STEC O-Type Kit
- Integrated internal control to ensure successful testing
- Screen virulence genes with Screen Plus Kit before testing with STEC O-Type Kit
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- Product Details
- Specifications
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Cat No./ID:290233 mericon E. coli STEC O-Type Kit (24) $246.00 For 24 reactions: PCR Assay E. coli O157 STEC O-Type, Internal Control, Multiplex PCR Master Mix, RNase-Free Water |
Cat No./ID:290405 mericon E. coli O157 Screen Plus Kit (96) Inquire For96 reactions: PCR Assay E. coli O157 Screen Plus, Internal Control, Multiplex PCR Master Mix, RNase-Free Water |
Product Details
The mericon E. coli Screen Plus Kit is designed for the qualitative detection of the E. coli serotype O157 and E. coli virulence genes intimin (eae) and Shiga toxin-like proteins (stx1 and stx2). The presence of eae and stx1/stx2 suggests that further investigation into the E. coli O-serotype O157, or the non-O157 O-serotypes (O26, O45, O103, O111, O121, and O145) is required in food and animal feed samples after enrichment. The assay can detect as few as 10 copies of E. coli DNA in a reaction and it exhibits high specificity for pathogens of this species. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see table "Results of mericon E. coli O157 Screen Plus Kit cross-reactivity study" for exclusivity/inclusivity data).The mericon E. coli STEC O-Type Kit exhibits high specificity for E. coli strain O157:H7, O26, O45, O103, O111, O121, and O145. The kit was tested against an extensive inclusivity panel of E. coli O157:H7 and non-O157:H7 serotype strains, and an exclusivity panel of other E. coli and non-E. coli strains. All tested O157:H7 and non-O157:H7 serotype strains (O26, O45, O103, O111, O121, and O145) were successfully detected and no cross-reactivity was observed with other pathogens using 2500 copies of tested DNA (see table "Results of mericon E. coli STEC O-Type Kit cross-reactivity study").
| Pathogen/gene | Result | Pathogen/gene | Result |
|---|---|---|---|
| eae | + | Enterobacter sakazakii | – |
| E. coli O157:H7 | + | Escherichia coli O6 | – |
| VTEC stx1 | + | Listeria monocytogenes | – |
| VTEC stx2 | + | Shigella flexneri | – |
| Bacillus cereus | – | Staphylococcus aureus | – |
| Camplyobacter jejuni | – | Yersinia enterocolitica | – |
| Clostridium perfringens | – |
| Pathogen | Strain no. | ResultOrange channel | ResultGreen channel |
|---|---|---|---|
| E. coli O26:H– | DSM 8695 | – | + |
| E. coli O45 | SSI 81886 | – | + |
| E. coli O103 | NC09103 | – | + |
| E. coli O103 | SSI 82109 | – | + |
| E. coli O111:H– | DSM 8698 | – | + |
| E. coli O111:H– | DSM 10234 | – | + |
| E. coli O121 | NC09121 | – | + |
| E. coli O145 | SSI 82280 | – | + |
| E. coli O157:H7 | DSM 8579 | + | – |
| E. coli O157:H7 | DSM 13526 | + | – |
| E. coli O157:H7 | DSM 17076 | + | – |
| E. coli O157:H7 | DSM 19206 | + | – |
| E. coli O1:H7 | DSM 30083 | – | – |
| E. coli O1:K1:H7 | ATCC11775 | – | – |
| E. coli O1:K1:H7 | DSM 10750 | – | – |
| E. coli O2:K1:H4 | DSM 10777 | – | – |
| E. coli O7:K1:H7 | DSM 10858 | – | – |
| E. coli O11:H10 | DSM 11751 | – | – |
| E. coli O12:H2 | DSM 8703 | – | – |
| E. coli O13:H11 | DSM 1058 | – | – |
| E. coli O18ac:K1:H7 | DSM 10723 | – | – |
| E. coli O19:H7 | DSM 11752 | – | – |
| E. coli O25:H4 | DSM 22664 | – | – |
| E. coli O29:H10 | DSM 9026 | – | – |
| E. coli O104 | NC09104 | – | – |
| E. coli O107:H5 | DSM 9033 | – | – |
| E. coli O112ac:H– | DSM 9027 | – | – |
| E. coli O113 | SSI 82121 | – | – |
| E. coli O124:H30 | DSM 9031 | – | – |
| E. coli O126:H2 | DSM 8701 | – | – |
| E. coli O143:H– | DSM 9028 | – | – |
| E. coli RM-HYG118 | VTEC isolate | – | – |
| E. coli RM-HYG119 | VTEC isolate | – | – |
| E. coli RM-HYG120 | VTEC isolate | – | – |
| Bacillus cereus | ATCC 11778 | – | – |
| Campylobacter jejuni | ATCC 33560 | – | – |
| Clostridium perfringens | ATCC 13124 | – | – |
| Enterobacter sakazakii | DSM 4485 | – | – |
| Escherichia coli | ATCC | – | – |
| Escherichia coli | NCTC 9002 | – | – |
| Legionella erythra | Sifin | – | – |
| Listeria monocytogenes | ATCC 7644 | – | – |
| Shigella flexneri | ATCC 12022 | – | – |
| Staphylococcus aureus | ATCC 25923 | – | – |
| Vibrio vulnificus | ATCC 43478 | – | – |
| Yersinia enterocolitica | ATCC 9610 | – | – |
According to national regulations such as the USDA FSIS regulations for the US beef industry, all non-intact products (beef trimmings) are required to be tested, or should be tested, for 6 strains of shiga toxigenic E. coli (STEC) — the "big 6" — before they can be shipped to the food distribution chain.
The mericon E. coli testing workflow provides a complete STEC screening and confirmation solution for a variety of food products. The assays are run on QIAGEN Rotor-Gene Q real-time PCR instruments able to detect at least 4 channels: Rotor-Gene Q 5plex, Rotor-Gene Q 5plex HRM, and Rotor-Gene Q 6plex. The workflow detects the "big 6" STECs, as well as E. coli O157:H7. The first step of the workflow —screening for potentially positive samples —is carried outusing the mericon E. coli O157 Screen Plus Kit. The second step of the workflow —identification of E. coli O157:H7 and distinction from other non-O157 O-type strains —is carried outusing the mericon E. coli STEC O-Type Kit.
The mericon E. coli O157 Screen Plus Kitis designed for the qualitative detectionof the E. coli serotype O157 and E. coli virulence genes intimin (eae) and Shiga toxin-like proteins (stx1 and stx2). The presence of eae and stx1/stx2 suggests that further investigation into the presence of E. coli O-serotype O157, or the non-O157 O-serotypes (O26, O45, O103, O111, O121, and O145) is required in food and animal feed after enrichment. Themericon E. coli O157 Screen Plus Kitis designedto alloweasy decision making in the next stepof the workflow and interpretation can be summarized by a simple table (see the table "Interpretation of results with the mericon E. coli O157 Screen Plus Kit").
| No. | OrangeO157 | Green stx1/stx2 | Crimson eae | Yellow IC | Next action |
|---|---|---|---|---|---|
| 1 | – | – | – | Valid | No further action |
| 2 | – | + | – | Valid | No further action |
| 3 | – | – | + | Valid | No further action |
| 4 | + | – | – | Valid | Additional testing required |
| 5 | – | + | + | Valid | Additional testing required |
| 6 | + | + | + | Valid | Additional testing required |
| 7 | – | – | – | Invalid | Dilute sample and repeat test |
| No. | O157:H7 Orange channel | Non-O157:H7 serotypes Green channel | IC Yellow channel | Next action |
|---|---|---|---|---|
| 1 | – | – | Valid | No further action |
| 2 | – | + | Valid | Additional testing required |
| 3 | + | – | Valid | Additional testing required |
| 4 | + | + | Valid | Additional testing required |
| 5 | – | – | Invalid | Dilute sample and repeat test |
DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit or the QIAsymphony mericon Bacteria Kit are recommendedfor the isolation of bacterial DNA from Gram-negative bacteria.
The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol thatdelivers results in 73 minutes on QIAGEN"s Rotor-Gene Q.
Results are displayed as easy-to-interpret PCR curves.
Specifications
Features | Specifications |
| Quantitative/qualitative | Qualitative |
| Recommended sample prep | mericon DNA Bacteria Kit or QIAsymphony mericon DNA Bacteria Kit (Gram-negative bacteria) |
| RUO/CE/ASR | RUO |
| Sample type | Bacterial preculture from food |
| Thermal cycler | Rotor-Gene Q 5plex, 5plex HRM, or 6plex |
| What detected | DNA of respective pathogen |
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