Qiagen/miRCURY LNA miRNA Custom PCR Assays/339317
市场价：
¥4780.00
美元价：
2868.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
miRCURY LNA miRNA Custom PCR Assays
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For SYBR® Green qPCR-based miRNA quantification using LNA-optimized miRNA PCR primer sets
- Design your own LNA-enhanced miRNA PCR primer sets for any miRNA
- Sensitive and specific detection and quantification of miRNA
- Fast and simple two-step protocol takes less than 3 hours
miRCURY LNA miRNA Custom PCR Assays are Custom-designed miRNA PCR primer sets that enable extremely sensitive and specific miRNA quantification with the miRCURY LNA miRNA PCR System. Both forward and reverse PCR amplification primers are miRNA-specific and are optimized with LNA technology. Each tube contains sufficient assays for 200 reactions.
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Cat No./ID:339317 miRCURY LNA miRNA Custom PCR Assay $239.00 Contains forward and reverse primers for 200 reactions; for SYBR® Green-based detection |
miRCURY LNA miRNA Custom PCR Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Unmatched sensitivity
Compared with other miRNA real-time PCR systems that use either stem-loop or standard DNA primers, the LNA-enhanced primers offer significantly increased sensitivity, especially for AT-rich miRNAs (see figure LNA-enhanced primers result in greatly increased sensitivity compared to DNA primers).The low sample requirements also enable miRNA quantitation using total RNA purified from difficult samples such as LCM samples and serum/plasma (see figuresDetection of differential expression of miRNAs in LCM specimens from FFPE tissue sections and Differences in miRNA expression between serum samples).Fast, easy and reproducible
The easy-to-follow, 3-hour protocol saves you bothtime and effort in the laboratory. By using the same RT reaction as the template in all subsequent PCR reactions, the procedure is greatly simplified compared with systems that require miRNA-specific first-strand synthesis. The number of pipetting steps is reduced to a minimum, and technical variation is minimized. This makes it possible to achieve extremely high reproducibility from day-to-day and even site-to-site.miRCURY LNA miRNA PCR Assays have been optimized for use with the miRCURY LNA RT Kit and the miRCURY LNA SYBR Green PCR Kit. Use of other reagents will affect the quality of the results.Principle
The design process
The miRCURY LNA miRNA Custom PCR Assay design tool lets you easily design highly sensitive and specific LNA-enhanced PCR primer sets for any miRNA not available as a predesigned product. The advanced algorithm evaluates approximately 3,000 primer pair designs based on more than 60 different criteria to find LNA optimal primer sets for your miRNA within a few minutes. The tool has been designed for miRNAs but can also be used for other small RNAs 14–27 nucleotides in length. The design criteria include:- Optimization of melting temperatures by varying the LNA distribution of the primers to ensure high amplification efficiency and specificity.
- Calculations and adjustments of self-hybridization and cross-hybridization scores for efficient PCR reactions.
- Adjustments to avoid potential primer–dimer formation in the PCR reaction.
- Intelligent positioning of LNA bases in the primers based on our vast knowledge of LNA oligonucleotide design.
- miRBase searches to identify potential miRNAs with high sequence similarity. This ensures that the primer sets are highly specific for the small RNA for which they were designed.
A unique system for miRNA profiling
miRCURY LNA miRNA PCR Assays offer the best combination of performance and ease-of-use available on the miRNA real-time PCR market by combining universal RT with LNA PCR amplification (see figure Schematic outline of the miRCURY LNA miRNA PCR System). Universal RT makes it possible to use one first-strand cDNA synthesis reaction as the template for multiple miRNA real-time PCR assays. This saves precious samples, reduces technical variation and saves time in the laboratory. Plus, both the forward and reverse PCR amplification primers are miRNA specific and optimized with LNA. This provides 1) exceptional sensitivity and extremely low background, enabling accurate quantitation of very low miRNA levels and 2) highly specific assays that allow discrimination between closely related miRNA sequences.Coverage
Over 20,000 assays are available covering all organisms in miRBase 20. Over 1,400 assays are fully wet-lab validated for sensitivity, specificity, efficiency and background on both synthetic as well as different biological samples. The remaining assays are in silico-validated using a comprehensive design algorithm that ensures high-quality, species-specific, LNA-enhanced assays with optimal sensitivity and specificity within each organism. This means that several different assays may target the same sequence. Ultimately, the assay for each species is selected based on the genetic background of the organism. If you are working with novel miRNAs, such as from an NGS experiment, custom-designed LNA miRNA primer sets for any miRNA are also available.Procedure
The miRCURY LNA miRNA PCR Assay system is an miRNA-specific, LNA-based system designed for sensitive and accurate detection of miRNA by quantitative real-time PCR using SYBR Green. The first step of the procedure is universal reversetranscription, followed by real-time PCR amplification with LNA-enhanced primers (see figure miRCURY LNA miRNA PCR System at a glance).
Applications
miRCURY LNA miRNA PCR Assays are used as part of the miRCURY LNA miRNA PCR System for:
- Mature miRNA quantification
- snoRNA detection
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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