mericon Ingredient Authentication Assays
For real-time PCR authentication of food and animal feed ingredients
- Highly sensitive and specific authentication of ingredients
- Single, streamlined protocol for all targets
- Dedicated mericon DNA isolation kit available
- PCR run time of 82 minutes on the QIAGEN Rotor-Gene Q
- Full license for PCR without additional costs
mericonIngredient AuthenticationAssays are a suite of kits for target-specificauthentication of food andanimal feed ingredients and certain pharmaceutical product components of animal and plant origin. All of the assays use the same real-time PCR-based protocol. They are part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits, and assays forpathogen and GMO DNA detection. mericonassays perform optimally on the Rotor-Gene Q, but have also been validated for block thermal cyclers.Please note: The product 293015:mericonSoy Kit (96) is phasing-out by December 31, 2015 and available only while stock lasts. Please contact your customer care department. Please use product 293013: mericon Soy Kit (24)as alternative.
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Cat No./ID:292013 mericon Pig Kit (24) $292.00 For 24 reactions: PCR Assay Pig, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water |
Cat No./ID:292015 mericon Pig Kit (96) $1,057.00 For 96 reactions: PCR Assay Pig, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water |
Cat No./ID:292025 mericon Cattle Kit (96) $1,057.00 For96 reactions: PCR Assay Cattle, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution |
Cat No./ID:292033 mericon Chicken Kit (24) $286.00 For 24 reactions: PCR Assay Chicken, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution |
Cat No./ID:292035 mericon Chicken Kit (96) $1,065.00 For96 reactions: PCR Assay Chicken, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution |
Product Details
mericon Apricot Kernels Kit
The mericon Apricot Kernels Kit is for the detection of matter from apricot kernels in food and animal feed. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for apricot DNA. In an experiment using 50 ng of tested DNA, no cross-reactivity was observed with matter from other plant and animal species (see the table "Results of mericon Apricot Kernels Kit cross-reactivity study").
mericon Cattle Kit
The mericon Cattle Kit is for the detection of matter of cattle origin in food and animal feed. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for cattle DNA. In an experiment using 50 ng of tested DNA, no cross-reactivity was observed with matter from other animal and plant species, including water buffalo (see the table "Results of mericon Cattle Kit cross-reactivity study").
mericon Chicken Kit
The mericon Chicken Kit is for the detection of matter of chicken origin in food and animal feed. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for chicken DNA. In an experiment using 50 ng of tested DNA, no cross-reactivity was observed with matter from other animal and plant species (see the table "Results of mericon Chicken Kit cross-reactivity study").
mericon Corn Kit
The mericon Corn Kit is for the detection of corn in food and animal feed. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for corn DNA. In an experiment using 50 ng of tested DNA, no cross-reactivity was observed with matter from other plant and animal species (see the table "Results of mericon Corn Kit cross-reactivity study").
mericon Pig Kit
The mericon Pig Kit is for the detection of matter originating from domestic pig (Sus scrofa domestica) and wild boar (Sus scrofa) in food and animal feed. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for pig and wild boar DNA. In an experiment using 50 ng of tested DNA, no cross-reactivity was observed with matter from other animal and plant species (see the table "Results of mericon Pig Kit cross-reactivity study").
mericon Soy Kit
The mericon Soy Kit is for the detection of soy (Glycine max) in food and animal feed. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for soy and Roundup Ready soy DNA. In an experiment using 50 ng of tested DNA, no cross-reactivity was observed with matter from other plant and animal species (see the table "Results of mericon Soy Kit cross-reactivity study").
mericon Turkey Kit
The mericon Turkey Kit is for the detection of matter of turkey origin in food and animal feed. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for turkey DNA. In an experiment using 50 ng of tested DNA, no cross-reactivity was observed with matter from other animal and plant species (see the table "Results of mericon Turkey Kit cross-reactivity study").
| Species | Result | Species | Result |
|---|---|---|---|
| Apricot | + | Rice | – |
| Almond | – | Potato | – |
| Peach | – | Barley | – |
| Plum | – | Wheat | – |
| Sargent cherry | – | Rye | – |
| Cherry | – | Cattle | – |
| Hazelnut | – | Pig | – |
| Peanut | – | Sheep | – |
| Walnut | – | Goat | – |
| Pecan nut | – | Horse | – |
| Corn | – | Lupine | – |
| Canola | – | Celery | – |
| Soy | – | Mustard | – |
| Species | Result | Species | Result |
|---|---|---|---|
| Cattle | + | Deer | – |
| Water buffalo | – | Cod | – |
| Turkey | – | Salmon | – |
| Chicken | – | Rabbit | – |
| Goose | – | Corn | – |
| Duck | – | Soy | – |
| Pig | – | Rice | – |
| Sheep | – | Wheat | – |
| Goat | – | Potato | – |
| Horse | – |
| Species | Result | Species | Result |
|---|---|---|---|
| Chicken | + | Deer | – |
| Turkey | – | Cod | – |
| Goose | – | Water buffalo | – |
| Duck | – | Salmon | – |
| Ostrich | – | Rabbit | – |
| Cattle | – | Corn | – |
| Pig | – | Soy | – |
| Sheep | – | Rice | – |
| Goat | – | Wheat | – |
| Horse | – | Potato | – |
| Species | Result | Species | Result |
|---|---|---|---|
| Corn | + | Hazelnut | – |
| Soy | – | Pecan nut | – |
| Canola | – | Brazil nut | – |
| Rice | – | Almond | – |
| Potato | – | Pig | – |
| Barley | – | Cattle | – |
| Wheat | – | Sheep | – |
| Rye | – | Goat | – |
| Lupine | – | Horse | |
| Mustard |
| Species | Result | Species | Result |
|---|---|---|---|
| Domestic pig | + | Chicken | – |
| Wild boar | + | Sheep | – |
| Turkey | – | Goat | – |
| Duck | – | Cattle | – |
| Goose | – | Horse | – |
| Deer | – | Ostrich | – |
| Kangaroo | – | Reindeer | – |
| Moose | – | Cod | – |
| Salmon | – |
| Species | Result | Species | Result |
|---|---|---|---|
| Soy | + | Wheat | – |
| Roundup Ready soy | + | Corn | – |
| Potato | – | MON 810 corn | – |
| Rye | – | CGH351 corn | – |
| Barley | – | Bt176 corn | – |
| Rice | – | CaMV | – |
| Canola | – | Cattle | – |
| Roundup Ready rice | – | Goat | – |
| T25 corn | – | Pig | – |
| NK603 corn | – | Sheep | – |
| Bt11 corn | – | Horse | – |
| Species | Result | Species | Result |
|---|---|---|---|
| Turkey | + | Deer | – |
| Chicken | – | Cod | – |
| Goose | – | Water buffalo | – |
| Duck | – | Salmon | – |
| Ostrich | – | Rabbit | – |
| Cattle | – | Corn | – |
| Pig | – | Soy | – |
| Sheep | – | Rice | – |
| Goat | – | Wheat | – |
| Horse | – | Potato | – |
Authenticating ingredients using real-time PCR is based on the amplification of a specific region of the relevant target genome. The amplified product is detected using target-specific fluorescent probes that bind to the amplified product. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes after the PCR run.
mericon PCR Assays include an internal control to control potential inhibiton. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").
The PCR primer set for each assay is highly specific and targets either a genetic construct that is commonly used in GMOs or a DNA sequence that is specific for a particular GMO. These targets were verified bioinformatically and experimentally.
| Internal control amplified | Tested DNA amplified | Meaning |
|---|---|---|
| + | + | Sample is positive for the target DNA |
| + | – | Sample is negative for the target DNA |
| – | – | PCR failed due to inhibition |
DNA is extracted from a sample of food or animal feed using a dedicated sample preparation kit. QIAGEN"s DNeasy mericon Food Kit is suitable for a whole range of food matrices, including those with high inhibitor contents.
The isolated, pure DNA is assayed using a streamlined real-time PCR protocol that returns results in 82 minutes on QIAGEN"s Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.
The results are displayed as easy-to-interpret PCR curves.
mericon Ingredient Authentication Assays are for real-time PCR-based detection of DNA segments from specific animal or plant material in food, animal feed, and pharmaceutical products.
Specifications
Features | Specifications |
| Quantitative/qualitative | Qualitative |
| Recommended sample prep | DNeasy mericon Food Kit or DNeasy Plant Kits |
| RUO/CE/ASR | RUO |
| Sample type | Food |
| Thermal cycler | Rotor-Gene Q and other cyclers |
| What detected | DNA from soy and pig respectively |
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