RT2 SYBR® Green FAST Mastermixes
- Designed for 100-ring discRT2 Profiler /RT2 lncRNA PCR Arrays
- Available with ROX, fluorescein, or without dye
- Well-suited for use with self-designed PCR assays
- Exceptional qPCR performance in instruments with fast cycling conditions
RT2 SYBR Green FAST Mastermixes are specially formulated for use with the 100-ring disc format of theRT2 Profiler andRT2 lncRNA PCR arrays on the Rotor-Gene Q. This mastermix is suited for use with self-designed qPCR assays on other types of real-time PCR instruments with fast cycling conditions.RT2 SYBR Green FAST Mastermixes are available with ROX, fluorescein, or without reference dyes.
- Buy Products
- Product Details
Buy Products
Cat No./ID:330600 RT² SYBR Green FAST Mastermix (2) $204.00 Contains 2 x 1.35 ml tubes: for 2 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 250 x 20 µl reactions |
Cat No./ID:330601 RT² SYBR Green FAST Mastermix (8) $792.00 Contains 8 x 1.35mltubes: for 8 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1000 x 20 µl reactions |
Cat No./ID:330602 RT² SYBR Green FAST Mastermix (12) $1,128.00 Contains 12 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1500 x 20 µl reactions |
Cat No./ID:330603 RT² SYBR Green FAST Mastermix (24) $1,684.00 Contains 24 x 1.35 mltubes: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 3000 x 20 µl reactions |
Cat No./ID:330620 RT² SYBR Green ROX FAST Mastermix (2) $210.00 Contains 2 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 250 x 20 µl reactions |
Cat No./ID:330621 RT² SYBR Green ROX FAST Mastermix (8) $767.00 Contains 8 x 1.35 ml tubes: for 8 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1000 x 20 µl reactions |
Cat No./ID:330622 RT² SYBR Green ROX FAST Mastermix (12) $1,128.00 Contains 12 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1500 x 20 µl reactions |
Cat No./ID:330623 RT² SYBR Green ROX FAST Mastermix (24) $1,658.00 Contains 24 x 1.35 ml tubes: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 3000 x 20 µl reactions |
Cat No./ID:330629 RT² SYBR Green ROX FAST Mastermix (25 ml) $1,562.00 Contains 1 bottle of 25 ml mastermix: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 2500 x 20 µl reactions |
Product Details
RT² SYBR Green FAST Mastermixes contain all of the optimized reagents and buffers needed for SYBR Green based real-time PCR with RT2 qPCR Primer Assays and RT2 Profiler PCR Arrays on all real-time PCR instruments.
The high-performance real-time PCR enabled by RT² SYBR Green FAST Mastermixes demonstrates high amplification efficiencies (see figure "High amplification efficiency over a wide dynamic range") and high levels of sensitivity and specificity (see figure "Tighter control of polymerase activity yields greater specificity").
In addition, dissociation curves obtained using RT2 SYBR Green FAST Mastermixes have lower background and a sharper shape compared with dissociation curves generated by regular cycling conditions (see figure "Sharper dissociation curves with RT2 SYBR Green Fast Mastermix").
RT2 SYBR Green FAST Mastermixes contain real-time PCR buffer, a high-performance, HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. Some mastermixes contain either fluorescein or ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate SYBR Green results by preventing the amplification of primer–dimers and other nonspecific products. RT2 SYBR Green FAST Mastermixes are optimized to use a 10-second denaturation step and 30-second annealing/extension step PCR protocol, without compromising specificity or sensitivity.
Mastermixes without ROX or fluorescein
RT2 SYBR Green FAST Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation not requiring a reference dye, including Bio-Rad models CFX96, CFX384; Bio-Rad/MJ Research Chromo4, DNA Engine Opticon, DNA Engine Opticon 2; Roche LightCycler 480 (96-well and 384-well); Eppendorf Mastercycler ep realplex without ROX filter set; and Cepheid SmartCycler.
Mastermixes with fluorescein
RT2 SYBR Green Fluor FAST Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses fluorescein as a reference dye, including the Bio-Rad models iCycler, iQ5, MyiQ, and MyiQ2.
Mastermixes with ROX
RT2 SYBR Green ROX FAST Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses ROX as a reference dye, including QIAGEN’s Rotor-Gene Q; Applied Biosystems models 5700, 7000, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast 96-well block, 384-well block), StepOnePlus, ViiA 7 (Standard and Fast 96-well block, 384-well block); Eppendorf Mastercycler ep realplex with or without ROX filter set; Stratagene models Mx3000P, Mx3005P, Mx4000; and Takara TP-800.
Product Resources
Customers who bought these products also bought
- Cat No./ID:330404
RT2 First Strand Kit (50)
For fifty 20 µl first strand cDNA synthesis reactions; Buffer GE (100 µl), 5x Buffer BC3 (200 µl), RE3 Reverse Transcriptase Mix (100 µl), Control P2 (50 µl), Nuclease-Free Water (1 ml)$506.00Add To Cart - Cat No./ID:1027415
FlexiTube siRNA (1 nmol)
1 nmol siRNA delivered in tubesSelect Targets - Cat No./ID:330411
RT² HT First Strand Kit (96)
RT2 HT First Strand Kit$594.00Add To Cart - Cat No./ID:330401
RT2 First Strand Kit (12)
Fortwelve 20 µl first strand cDNA synthesis reactions; Buffer GE (24 µl), 5x Buffer BC3 (48 µl), RE3 Reverse Transcriptase Mix (24 µl), Control P2 (12 µl), Nuclease-Free Water (1 ml)$248.00Add To Cart - Cat No./ID:218075
miScript SYBR Green PCR Kit (1000)
For 1000 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer$2,068.00Add To Cart - Cat No./ID:330001
RT2 qPCR Primer Assay (200)
RT2 qPCR Primer AssaySelect Targets - Cat No./ID:47016
DNeasy PowerSoil Pro Kit (250)
For the isolation of microbial genomic DNA from all soil types.$1,614.00Add To Cart - Cat No./ID:80284
AllPrep DNA/RNA Micro Kit
For 50 micropreps: AllPrep DNA Spin Columns, RNeasy MinElute Spin Columns, Collection Tubes, Carrier RNA, RNase-Free Water and Buffers$766.00Add To Cart - Cat No./ID:203443
HotStarTaq Master Mix Kit (250 U)
3 x 0.85 ml HotStarTaq Master Mix (contains 250 units HotStarTaq DNA Polymerase, PCR Buffer with 3 mM MgCl2, and 400 µM of each dNTP)and 2 x 1.7 ml RNase-Free Water$227.00Add To Cart - Cat No./ID:73224
RNeasy Protect Animal Blood Kit (50)
For 50 preps: RNeasy MinElute Spin Columns, QIAshredder Spin Columns, Collections Tubes, ProteinaseK, RNase-Free DNase, and RNase-Free Reagents and Buffers; to be used with RNAprotect Animal Blood Tubes$520.00Add To Cart - Cat No./ID:981106
Strip Tubes and Caps, 0.1 ml (2500)
10 x 250 strips of 4 tubes and caps for 10,000 reactions of 10–50 µl$2,039.00Add To Cart - Cat No./ID:970902
PyroMark Q24 Advanced Reagents (4 x 24)
Enzyme mix, substrate mix, nucleotides, and buffers for Pyrosequencing assays; intended for use with PyroMark Q24 Advanced$376.00Add To Cart - Cat No./ID:330701
RT2 lncRNA qPCR Assay
For 200 reactions; mix of 2 gene-specific primers provided in solution (200 µl), with 10 µM of each primerSelect Targets - Cat No./ID:990522
200 µl Conductive Filtered Tips
Carbon-impregnated conductive tips (960 tips) for use with liquid-level sensing; tips contain high-set filters; for use with Tip Rack Holder (cat. no. 9018949)$176.00Add To Cart - Cat No./ID:55114
QIAamp Circulating Nucleic Acid Kit (50)
For 50 preps: QIAamp Mini Columns, Tube Extenders (20 ml), QIAGEN Proteinase K, Carrier RNA, Buffers, VacConnectors, and Collection Tubes (1.5 ml and 2 ml)$1,158.00Add To Cart - Cat No./ID:79656
QIAshredder (250)
250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps$406.00Add To Cart - Cat No./ID:929551
QX DNA Size Marker FX174/HaeIII (50 ul)
DNA size marker with fragments of 72, 118, 194, 234, 271, 281, 310, 603, 872, 1078, and 1353 bp; concentration 100 ng/ul$83.80Add To Cart - Cat No./ID:333714
QIAseq 12-Index I (48)
Box containing combinatorial dual-indexed adapters, for indexing up to 12 samples for QIAseq Targeted Panel sequencing on Illumina platforms; enough to process a total of 48 samples
$1,176.00Add To Cart - Cat No./ID:180473
QIAseq FX DNA Library Kit (24)
Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 24 combinatorial dual index adapters with different bar codes (pierceable foil seal, allowing usage of defined parts of plate)
$864.00Add To Cart - Cat No./ID:218161
miScript II RT Kit (50)
For 50 cDNA synthesis reactions: miScript Reverse Transcriptase Mix, 10x miScript Nucleics Mix, 5x miScript HiSpec Buffer, 5x miScript HiFlex Buffer, RNase-Free Water$522.00Add To Cart
