Qiagen/RT2PreAMP cDNA合成试剂盒/RT2Nano PreAMP cDNA合成试剂盒/330451
市场价：
¥10920.00
美元价：
6552.00
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3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
RT2 PreAMP cDNA Synthesis Kit
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For preamplification of cDNA from small or FFPE samples of RNA for analysis on RT2 Profiler PCR Arrays
- Preamplification from nanogram amounts of RNA
- Amplification of 89 gene-specific cDNA targets
- Minimal nonspecific reactions in downstream PCR arrays
The RT2 PreAMP cDNA Synthesis Kit is designed for preamplification of cDNA from nanogram amounts of RNA (1–100 ng) specifically for analysis using RT2 Profiler PCR Arrays.
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Cat No./ID:330451 RT2 PreAMP cDNA Synthesis Kit (12) $546.00 RT2 Nano PreAMP cDNA Synthesis Kit |
The RT2 PreAMP cDNA Synthesis Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Principle
RT² PreAMP technology utilizes multiplex, tandem PCR to preamplify gene-specific cDNA with minimal bias. The RT2 PreAMP cDNA Synthesis Kit is designedfor preamplification of first strand cDNA from 1–100 ng total RNA. Following standard reverse transcription, the kit works with an array-specific RT2 PreAMP Pathway Primer Mix to preamplify an array-specific set of targets from the cDNA before gene expression analysis using an RT² Profiler PCR Array. Each RNA sample will generate sufficient cDNA for gene expression analysis on up to 4 different RT² Profiler PCR Arrays.
Procedure
The procedurecomprises 2 simple steps:
- First strand cDNA synthesis: The kit can beused to synthesizecDNA from up to 12 different RNA samples.The built-in external RNA control template, which is detected by the reverse transcription control (RTC) assays in the RT² Profiler PCR Arrays, allows the detection of reverse transcription inhibitorsandensures efficient first strandsynthesis.
- Preamplification of cDNA for pathway-specific genes: Each first strand cDNA synthesis reaction from 1–100 ng of total RNA can be amplified using up to 4 different sets of PCR array-specific primer mixes, allowing gene expression analysis on up to 4 different RT² Profiler PCR Arrays. The Side Reaction Reducer included in the kit eliminates the residual primers from preamplification, enabling accurate detection on PCR arrays.
Applications
cDNA templates generated using the RT2 PreAMP cDNA Synthesis Kit in conjunction with RT2 PreAMP Pathway Primer Mixes are ready for gene expression profiling using RT2 Profiler PCR Arrays.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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