Qiagen/mericonDNA细菌（Plus）试剂盒/快速裂解缓冲液/69525
市场价：
¥2460.00
美元价：
1476.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
mericon DNA Bacteria (Plus) Kit
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For extraction of high-quality DNA from microbial enrichment culture
- Fast and efficient lysis of both Gram-negative and Gram-positive bacteria
- Protocols based on mechanical and thermal lysis
- Efficient removal of PCR inhibitors
The mericon DNA Bacteria Kit and mericon DNA Bacteria Plus Kit are designed to enable extraction of high-quality nucleic acids from Gram-positive and Gram-negative bacteria. The kits form part of the comprehensive QIAGEN food testing portfolio, which also features assays for pathogen and GMO DNA detection and ingredient authentication.
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- Product Details
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Cat No./ID:69525 mericon DNA Bacteria Kit (100) $123.00 Fast Lysis Buffer |
Cat No./ID:69534 mericon DNA Bacteria Plus Kit (50) $124.00 50 Pathogen Lysis Tubes L, Fast Lysis Buffer |
The mericon DNA Bacteria Kit and mericon DNA Bacteria Plus Kits are intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The thermal lysis procedure using the mericon DNA Bacteria Kit is optimized for the majority of easily lysed Gram-negative bacteria (e.g., Salmonella spp., Camplylobacter spp., and Cronobacter spp.), whereas the advanced features of the mechanical sample preparation using the mericon DNA Bacteria Plus Kit create the lysis conditions needed for difficult Gram-positive bacteria, such as Listeria spp. (see Figure "Efficient DNA extraction from Gram-negative or Gram-positive bacteria").
Principle
To date, the predominant method to test food for the presence of pathogens has been via culture preparation and/or ELISA-based assays. Molecular-based methods, specifically real-time PCR, offer an informative complement to these well-established methods. In addition, real-time PCR detection delivers results in less time (see Figure "Comparison of time to result of different methods for pathogen detection"), affords greater sensitivity and specificity, and has the robustness to cope with difficult food matrices (see Figure "Highly sensitive pathogen detection"). Finally, real-time PCR is a safe method for detecting challenging pathogen strains.
Procedure
To provide a streamlined and straightforward method for microbial sample preparation from pathogen enrichment cultures, QIAGEN has designed protocols based on mechanical lysis (using beads) and thermal lysis (by boiling) that deliver optimal disruption force for different bacteria types.
Thermal lysis is highly suited for the majority of easily lysed Gram-negative bacteria, whereas mechanical sample preparation creates the lysis conditions needed for difficult-to-lyse Gram-positive bacteria.
Carefully formulated chemistry included in the mericon DNA Bacteria Kit and the mericon DNA Bacteria Plus Kit maximizes lysis efficiency, stabilizes the extracted DNA, and removes inhibitors that may interfere with downstream applications.
Applications
The mericon DNA Bacteria and mericon DNA Bacteria Plus Kits are designed for the extraction of total genomic DNA from Gram-negative bacteria (mericon DNA Bacteria Kit) and Gram-positive bacteria (mericon DNA Bacteria Plus Kit) from pathogen enrichment cultures.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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