Qiagen/qBiomarker Copy Number PCR Assays/337812
市场价：
¥3440.00
美元价：
2064.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
qBiomarker Copy Number PCR Assays
Print
For locus-specific analysis of copy number variations and alterations
- Over 10 million assays available, targeting all regions of the genome
- Wet bench-tested assays
- Simple real-time PCR procedure
- Complimentary web-based data analysis Software
qBiomarker Copy Number PCR Assays provide a method for obtaining specific, accurate, reproducible, and easy-to-interpret copy number change results for an individual gene or region of interest (GOI or ROI). Every assay has been bench-verified and is ready-to-use in microarray follow-up studies, specific target screening, and related studies.
- Buy Products
- Product Details
Buy Products
Cat No./ID:337812 qBiomarker Copy Number PCR Assays $172.00 Laboratory-verified qPCR assays for measuring changes in copy number |
qBiomarker Copy Number PCR Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
All qBiomarker Copy Number PCR Assays are wet bench-tested for several characteristics affecting the accuracy of real-time PCR results: specificity, wide dynamic range, and uniformly high amplification efficiency. Laboratory verification of assay quality ensures that qBiomarker Copy Number PCR Assays deliver reliable results.
qBiomarker Copy Number PCR Assays accurately identified aneuploidy in cell lines containing chromosomal aberrations previously identified by cytogenetic methods. Assays forAR and MECP2, which are both on theX chromosome, correctly quantified gene copy number in cell lines with 1, 3, and 4 copies of the X chromosome.
Principle
All qBiomarker Copy Number PCR Assays are designed in unique regions of the genome. The qBiomarker Multicopy Reference Copy Number PCR Assay (MRef), which can be purchased separately, provides superior normalization and is recommended for use with the qBiomarker Copy Number PCR Assays. The MRef assay recognizes a stable sequence that appears in the human genome over 40 times, and whose copy number is not affected or minimally affected by local genomic changes. Inclusion of this reference assay during testing allows use of the ΔΔCT method to accurately make copy number calls or relative copy number change calls for specific targets. The all-in-one, ready-to-use qBiomarker SYBR® Green PCR Mastermixes are optimized to provide maximum locus-specific amplification with minimal primer dimers.
Procedure
Isolate genomic DNA from fresh, frozen, or FFPE samples. Combine an aliquot of each sample with the appropriate ready-to-use qBiomarker SYBR Green Mastermix and qBiomarker Copy Number PCR Assay. In another tube, mix an aliquot of each sample with the mastermix and the qBiomarker Multicopy Reference Copy Number PCR Assay. Run in a real-time cycler and determine copy number changes by the ΔΔCT method by comparing the test sample with a reference genome using the complimentary data analysis tool.
Applications
qBiomarker Copy Number PCR Assays are highly suited for accurate detection of copy number alterations or variations at individual loci using fresh, frozen, or FFPE samples.
Product Resources
Customers who bought these products also bought
- Cat No./ID:337820
qBiomarker SYBR Green ROX Mastermix (2)
For two 96-well qBiomarker Copy Number PCR Arrays or two-hundred-and-fifty 25 µl reactions$249.00Add To Cart - Cat No./ID:337822
qBiomarker SYBR Green ROX Mastermix (12)
For twelve 96-well qBiomarker PCR Arrays$1,310.00Add To Cart - Cat No./ID:19781
QIAfilter Mega-Giga Cartridges (5)
5 QIAfilter Mega-Giga Cartridges, Buffer FWB2$172.00Add To Cart - Cat No./ID:19112
Buffer MTL (54 ml)
Buffer MTL (54 ml)$40.50Add To Cart - Cat No./ID:75144
RNeasy Midi Kit (50)
50 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents and Buffers$708.00Add To Cart - Cat No./ID:205111
Omniscript RT Kit (50)
For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water$288.00Add To Cart - Cat No./ID:330001
RT2 qPCR Primer Assay (200)
RT2 qPCR Primer AssaySelect Targets - Cat No./ID:73504
RNeasy FFPE Kit (50)
50 RNeasy MinElute Spin Columns, Collection Tubes, Proteinase K, RNase-Free DNase I, DNase Booster Buffer, RNase-Free Buffers, RNase-Free Water$468.00Add To Cart - Cat No./ID:205311
QuantiTect Rev. Transcription Kit (50)
For 50 x 20 µl reactions: 100 µl 7x gDNA Wipeout Buffer, 50 µl Quantiscript Reverse Transcriptase, 200 µl 5x Quantiscript RT Buffer, 50 µl RT Primer Mix, 1.9 ml RNase-Free Water$391.00Add To Cart - Cat No./ID:19101
RNase A (17,500 U)
2.5 ml (100 mg/ml; 7000 units/ml, solution)Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.$226.00Add To Cart - Cat No./ID:74134
RNeasy Plus Mini Kit (50)
For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers$374.00Add To Cart - Cat No./ID:69504
DNeasy Blood & Tissue Kit (50)
50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)$171.00Add To Cart - Cat No./ID:79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
