Qiagen/RT2 lncRNA PCR Arrays/330721
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商品介绍
RT2 lncRNA PCR Arrays
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For lncRNA expression analysis by pathway and disease using laboratory-verified SYBR® Green qPCR assays
- Enables expression analysis of multiple related lncRNAs at one time
- Use for screening, or verification of RNA-seq and microarray studies
- All qPCR assays laboratory-verified for qPCR performance
- Available for 384- and 96-well plate and 100-well ring disc formats
RT2 lncRNA PCR Arrays combine real-time PCR performance and a panel of long noncoding RNAs related to a disease state or biological pathway to deliver sensitive, specific lncRNA profiling. These arrays are especially suited for researchers who prefer RT-PCR technology and require the capacity to study the expression of multiple lncRNAs at once.
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Cat No./ID:330721 RT2 lncRNA PCR Array Go to GeneGlobePCR arrays for pathway or disease profiling |
RT2 lncRNA PCR Arrays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Principle
RT2 lncRNA PCR Arrays are panels of RT2 lncRNA qPCR Assays for long noncoding RNAs related by pathway or disease. Assays are designed using a computer algorithm which has been developed using an in vitro assay to ensure that the resulting primer sequences generate a single PCR product of the predicted size without primer-dimers or off-target amplification in 33 cycles of PCR amplification. The assay also ensures that the amplification efficiency of the primers is 100 +/– 10%. As a result, the algorithm designs highly effective primer sequences for SYBR Green based real-time PCR detection. For optimal performance, RT2 lncRNA PCR Arrays should be used together with the RT2 First Strand Kit for cDNA synthesis and RT2 SYBR Green Mastermixes for qPCR. These reagents have been formulated and pretested together with RT2 lncRNA PCR Arrays. The RT2 First Strand Kit includes a proprietary genomic DNA elimination step to remove any residual contamination in RNA samples before reverse transcription, thereby eliminating false positive signals. In addition, an artificial RNA control template is also included which can be used to follow the reverse transcription efficiency across samples and monitor against RNase contamination. Each of the real-time instrument-specific RT2 SYBR Green Mastermixes contains SYBR Green and an appropriate reference dye to match the instrumentation available in your laboratory. RT2 SYBR Green Mastermixes are available for all real-time PCR instruments from QIAGEN, Applied Biosystems, Bio-Rad, Stratagene, Eppendorf, Roche, and other major suppliers.
Procedure
Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. RT2 lncRNA PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche, and Stratagene instruments. Flexible layout and controlsRT2 lncRNA PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and are used to monitor the expression of 84 lncRNA genes related to a disease state or pathway, plus 5 reference genes. Each RT2 lncRNA PCR Array also includes control elements for:
- Data normalization
- Genomic DNA contamination detection
- RNA sample quality
- General PCR performance
Applications
RT2 lncRNA PCR Arrays are highly suited for biomarker studies and other studies that require simultaneous, sensitive analysis of lncRNA expression.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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