QuantiTect SYBR® Green PCR Kits
For real-time PCR and two-step RT-PCR using SYBR Green
- High PCR specificity with integrated hot start
- Reliable quantification of low-abundance transcripts
- Accurate quantification over several logs of template
- Available with or without uracil-N-glycosylase (UNG)
- No need to optimize reaction and cycling conditions
The QuantiTect SYBR Green PCR Kit provides highly specific quantification of gDNA and cDNA targets by real-time PCR and two-step RT-PCR using SYBR Green I detection. The combination of a hot start and a unique qPCR buffer system ensures highly specific and sensitive real-time quantification of gDNA and cDNA targets. The dNTP mix includes dUTP, allowing optional treatment with UNG. For convenience, the master mix can be stored at 2–8°C.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:204143 QuantiTect SYBR Green PCR Kit (200) $459.00 For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water |
Cat No./ID:204145 QuantiTect SYBR Green PCR Kit (1000) $1,890.00 For 1000 x 50 µl reactions: 25 ml 2x QuantiTect SYBR Green PCR Master Mix, 20 ml RNase-Free Water |
Cat No./ID:204163 QuantiTect SYBR Green PCR +UNG Kit (200) $546.00 For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 100 µl UNG Solution, 2 x 2 ml RNase-Free Water |
Product Details
QuantiTect SYBR Green PCR Kits allow specific quantification over a wide linear range (see figure "Specific quantification over a wide linear range"). HotStarTaq DNA Polymerase provides the most stringent hot start compared with other polymerases, further increasing the specificity of the reaction (see figure "Highly specific amplification"). The unique composition of PCR buffer and HotStarTaq DNA Polymerase enable quantification of even low-abundance transcripts — as few as 5 copies of a target can be accurately detected (see figure "Highly sensitive quantification"). When used in combination with the QuantiTect Reverse Transcription Kit and QuantiTect Primer Assays, the QuantiTect SYBR Green PCR Kit delivers sensitive and reliable results (see figure "Specific and sensitive quantification").
The combination of the specially optimized UNG solution and the proven PCR master mix in the QuantiTect SYBR Green PCR +UNG Kit ensures effective elimination of carried-over PCR products together with reliable quantification of target sequences (see figure "High specificity in real-time PCR").
QuantiTect SYBR Green PCR Kits contain an optimized, ready-to-use master mix for highly specific and sensitive real-time quantification cDNA targets using SYBR Green I. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target-specific labeled probes. A balanced combination of K+ and NH4+ ions in the PCR buffer promotes specific primer annealing and enables high PCR specificity and sensitivity (see figure "Specific primer annealing"). In addition, HotStarTaq DNA Polymerase provides a stringent hot start, preventing the formation of nonspecific products.
QuantiTect SYBR Green PCR master mix also contains dUTP, enabling pretreatment with uracil-N-glycosylase (UNG) prior to starting PCR, which ensures that any contaminating PCR products do not affect subsequent PCR reactions.
| Component | Features | Benefits |
|---|---|---|
| HotStarTaq DNA Polymerase | 15 min activation at 95ºC | Set-up of qPCR reactions at room temperature |
| QuantiTect SYBR Green PCR Buffer | Balanced combination of NH4+ and K+ ions | Specific primer annealing ensures reliable PCR results |
| dNTP mix | Includes dUTP, which partially replaces dTTP and enables optional UNG treatment of reactions | Eliminates contamination from carryover of PCR products by optional UNG treatment |
| SYBR Green I dye | Yields a strong fluorescent signal upon binding double-stranded DNA | Highly sensitive quantification |
| ROX dye | For normalization of fluorescent signals on Applied Biosystems and, optionally, Agilent instruments | Precise quantification on cyclers that require ROX dye. Does not interfere with reactions on other real-time cyclers |
QuantiTect SYBR Green PCR Kits overcome the need for optimization of reaction conditions, which can be tedious and time-consuming. Simply add primers and DNA template to the ready-to-use PCR master mix, and start the reaction (see flowchart "Two-step RT-PCR"). Follow the protocol in the handbook to get fast and reliable results on any real-time cycler. If required, reactions can be pretreated with uracil-N-glycosylase (UNG) to eliminate carryover of PCR products from previous reactions.
For optimal results in real-time two-step RT-PCR, we recommend synthesizing cDNA using the QuantiTect Reverse Transcription Kit. The kit provides fast cDNA synthesis in just 20 minutes with integrated removal of genomic DNA contamination.
Highly specific results in gene expression analysis are guaranteed when QuantiTect SYBR Green PCR Kits are used in combination with QuantiTect Primer Assays. These are genomewide, bioinformatically validated primer sets for detecting transcripts from human, mouse, rat, and many other species. QuantiTect Primer Assays can be easily ordered online at GeneGlobe.
The QuantiTect SYBR Green PCR Kit is for use in gene expression analysis of cDNA targets and quantitative gDNA analysis. QuantiTect SYBR Green PCR Kits are compatible with all available real-time cyclers, including instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent. For the Rotor-Gene Q and other Rotor-Gene cyclers, we recommend using the Rotor-Gene SYBR Green PCR Kit, which has been specially developed for fast cycling on these instruments.
Specifications
Features | Specifications |
| Applications | Real-time quantification of genomic DNA or cDNA targets |
| Reaction type | Real-time PCR and two-step RT-PCR |
| Real-time or endpoint | Real-time |
| Sample/target type | DNA, cDNA |
| Single or multiplex | Single |
| SYBR Green I or sequence-specific probes | SYBR Green I |
| Thermal cycler | All real-time cyclers (e.g. LC, RG, ABI) |
| With or without ROX | With ROX |
Product Resources
Product citations in scientific articles
Customers who bought these products also bought
- Cat No./ID:205311
QuantiTect Rev. Transcription Kit (50)
For 50 x 20 µl reactions: 100 µl 7x gDNA Wipeout Buffer, 50 µl Quantiscript Reverse Transcriptase, 200 µl 5x Quantiscript RT Buffer, 50 µl RT Primer Mix, 1.9 ml RNase-Free Water$391.00Add To Cart - Cat No./ID:158489
Gentra Puregene Blood Kit Plus (1000 ml)
For 1000 ml blood: RBC Lysis Solution, RNase A Solution, and Reagents$1,638.00Add To Cart - Cat No./ID:204243
QuantiTect SYBR Green RT-PCR Kit (200)
For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green RT-PCR Master Mix, 100 µl QuantiTect RT Mix, 2 x 2 ml RNase-Free Water$932.00Add To Cart - Cat No./ID:205314
QuantiTect Rev. Transcription Kit (400)
For 400 x 20 µl reactions: 800 µl 7x gDNA Wipeout Buffer, 400 µl Quantiscript Reverse Transcriptase, 1.6 ml 5x Quantiscript RT Buffer, 400 µl RT Primer Mix, 8 x 1.9 ml RNase-Free Water$2,420.00Add To Cart - Cat No./ID:929500
QX Intensity Calibration Marker (1 ml)
1 ml QX Intensity Calibration Marker$81.40Add To Cart - Cat No./ID:218161
miScript II RT Kit (50)
For 50 cDNA synthesis reactions: miScript Reverse Transcriptase Mix, 10x miScript Nucleics Mix, 5x miScript HiSpec Buffer, 5x miScript HiFlex Buffer, RNase-Free Water$522.00Add To Cart - Cat No./ID:205313
QuantiTect Rev. Transcription Kit (200)
For 200 x 20 µl reactions: 4 x 100 µl 7x gDNA Wipeout Buffer, 4 x 50 µl Quantiscript Reverse Transcriptase, 4 x 200 µl 5x Quantiscript RT Buffer, 4 x 50 µl RT Primer Mix, 4 x 1.9 ml RNase-Free Water$1,300.00Add To Cart - Cat No./ID:204256
QuantiFast Probe PCR Kit (2000)
For 2000 x 25 µl reactions: 25 ml 2x QuantiFast Probe PCR Master Mix (contains ROX dye), 20 ml RNase-Free Water$2,033.00Add To Cart - Cat No./ID:74104
RNeasy Mini Kit (50)
50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers$330.00Add To Cart - Cat No./ID:158902
RBC Lysis Solution (450 ml)
450 ml RBC Lysis Solution$97.00Add To Cart - Cat No./ID:929704
QX Color 0.2 ml 12-Tube Strip (80)
80 x QX Color 0.2 ml 12-Tube Strips$118.00Add To Cart - Cat No./ID:249900
QuantiTect Primer Assay (200)
For 200 x 50 µl reactions or 400 x 25 µl reactions: 10x QuantiTect Primer Assay (lyophilized) supplied in single tubeSelect Targets - Cat No./ID:981311
Rotor-Disc 100 (30)
30 individually wrapped discs for 3000 reactions of 15–25 µl$375.00Add To Cart - Cat No./ID:204076
Rotor-Gene SYBR Green PCR Kit (2000)
For 2000 x 25 µl reactions: 25 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 20 ml RNase-Free Water$1,608.00Add To Cart - Cat No./ID:204074
Rotor-Gene SYBR Green PCR Kit (400)
For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water$471.00Add To Cart - Cat No./ID:981601
Rotor-Disc Heat Sealing Film (60)
60 films for sealing Rotor-Disc 100 or Rotor-Disc 72 discs$120.00Add To Cart - Cat No./ID:929520
QX Alignment Marker 15 bp/500 bp (1.5 ml)
Alignment marker with 15 bp and 500 bp fragments$97.00Add To Cart - Cat No./ID:210212
QIAGEN OneStep RT-PCR Kit (100)
For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)$615.00Add To Cart - Cat No./ID:239125
GelPilot Wide Range Ladder (100)
Ready-to-use DNA marker; 600 µl (100 lanes), 7 fragments: 200–4500 bp$148.00Add To Cart - Cat No./ID:9018901
Loading Block 72 x 0.1 ml Tubes
Aluminum block for manual reaction setup with a single-channel pipet in 72 x 0.1 ml tubes$345.00Add To Cart
