QuantiTect Virus Kits
- High sensitivity in single and multiplex assays
- Detection of viral RNA and/or DNA in the same reaction
- Clear detection of weak positive signals
- Fast universal 2-step protocols
- 5x master mix for higher sensitivity with more sample input
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:211011 QuantiTect Virus Kit (50) $254.00 Trial kit for 50 x 50 µl reactions: QuantiTect Virus Master Mix (contains ROX dye), QuantiTect Virus RT Mix, RNase-Free Water, QuantiTect Nucleic Acid Dilution Buffer |
Cat No./ID:211013 QuantiTect Virus Kit (200) $939.00 For 200 x 50 µl reactions: QuantiTect Virus Master Mix (contains ROX dye), QuantiTect Virus RT Mix, RNase-Free Water, QuantiTect Nucleic Acid Dilution Buffer |
Cat No./ID:211015 QuantiTect Virus Kit (1000) $3,684.00 For 1000 x 50 µl reactions: QuantiTect Virus Master Mix (contains ROX dye), QuantiTect Virus RT Mix, RNase-Free Water, QuantiTect Nucleic Acid Dilution Buffer |
Cat No./ID:211033 QuantiTect Virus +ROX Vial Kit (200) $939.00 For 200 x 50 µl reactions: QuantiTect Virus NR Master Mix (without ROX dye), ROX Dye Solution, QuantiTect Virus RT Mix, RNase-Free Water, QuantiTect Nucleic Acid Dilution Buffer |
Cat No./ID:211035 QuantiTect Virus +ROX Vial Kit (1000) $3,684.00 For 1000 x 50 µl reactions: QuantiTect Virus NR Master Mix (without ROX dye), ROX Dye Solution, QuantiTect Virus RT Mix, RNase-Free Water, QuantiTect Nucleic Acid Dilution Buffer |
Product Details
Amplification using QuantiTect Virus Kits provides steep sigmoidal curves over a range of dilutions, even for low template amounts with high CT values (see figure "Unambiguous determination of CT values over a wide dynamic range"). This enables accurate CT value determination for quantification of viral nucleic acids in real-time PCR.
Multiplex assays enable the detection of multiple viral RNA and/or DNA targets plus internal controls over a wide linear range without loss of sensitivity (see figures "Reliable detection of viral RNA over a wide linear range" and "Improved detection of low amounts of viral RNA").
QuantiTect Nucleic Acid Dilution Buffer, supplied with the kits, stabilizes RNA and DNA standards during dilution and reaction setup and prevents loss of nucleic acids on plastic surfaces, such as tubes or pipet tips. The buffer enables reliable dilution of standards used to quantify viral nucleic acids, giving a wide linear range, from low to high CT values and ensures longer storage of standards without degradation (see figure "Reliable dilution and storage of RNA standards").
QuantiTect Virus Kits provide highly sensitive detection of viral nucleic acids in single or multiplex assays on the first attempt (see flowchart "QIAGEN multiplex kits"). The optimized master mix ensures that PCR products in a multiplex reaction are amplified with the same efficiency and sensitivity as PCR products in a corresponding single-amplification reaction.
Amplifying control and target genes in the same reaction, instead of separate reactions, increases the reliability of gene quantification by minimizing handling errors. The QuantiTect Virus Buffer contains a balanced combination of K+ and NH4+ ions as well as unique synthetic Factor MP stabilizes, which together promote stable and efficient annealing of primers and probes to the nucleic acid template, enabling high PCR efficiency (see figure "Unique PCR buffer"). In addition, the unique formulation of Sensiscript Reverse Transcriptase ensures highly sensitive reverse transcription of viral RNA, while HotStarTaq Plus DNA Polymerase provides a stringent hot start, preventing the formation of nonspecific products.
| Kit component | Feature | Benefits | |
|---|---|---|---|
| 5x QuantiTect Virus Master Mix | Concentrated master mix | Highly concentrated and optimized for sensitive virus detection | Larger volumes of template can be added to the assay for increased sensitivity |
| HotStarTaq Plus DNA Polymerase | 5 min activation at 95ºC | Set up of qPCR reactions at room temperature | |
| QuantiTect Virus Buffer | Balanced combination of NH4+ and K+ ions | Specific primer annealing ensures reliable PCR results | |
| Synthetic Factor MP | Reliable multiplexing analysis of up to 4 genes in the same tube | ||
| Additional kit components | QuantiTect Virus RT Mix | Contains a unique formulation of Sensiscript Reverse Transcriptase | Optimized for highly sensitive detection of viral RNA |
| QuantiTect Nucleic Acid Dilution Buffer | Proprietary buffer formulation for dilution and storage of nucleic acid standards. | Stabilizes RNA and DNA standards during dilution and reaction setup and prevents loss of nucleic acids on plastic surfaces, such as tubes or pipet tips |
QuantiTect Virus Kits provide highly sensitive real-time PCR analysis of viral nucleic acids (RNA and/or DNA) and internal controls using sequence-specific probes. Reactions can be carried out with or without a reverse-transcription step, enabling flexible design of multiplex assays to detect RNA targets, DNA targets, or both RNA and DNA targets. Follow the protocol in the handbook for fast and reliable results.
Kits are available with or without ROX passive reference dye in the master mix (see table).
| ROX dye | Kit | Compatible cyclers |
|---|---|---|
| Supplied in master mix | QuantiTect Virus Kit | All cyclers from Applied Biosystems except Applied Biosystems 7500 |
| Supplied in separate tube | QuantiTect Virus +ROX Vial Kit | Applied Biosystems 7500 and cyclers fromBio-Rad, Cepheid, Eppendorf, QIAGEN, Roche, Agilent, and other suppliers |
For fast and highly sensitive end-point one-step RT-PCR applications, including virus detection, we recommend using the QIAGEN OneStep RT-PCR Kit.
Specifications
Features | Specifications |
| Applications | Virus detection |
| Reaction type | Reverse Transcription and PCR |
| Real-time or endpoint | Real-Time |
| Sample/target type | RNA and/or DNA targets |
| Single or multiplex | Single or multiplex |
| SYBR Green I or sequence-specific probes | Sequence-specific probes |
| Thermal cycler | Most real-time cyclers (except capillary cyclers e.g. LightCycler® 1.x and 2.0) |
| With or without ROX | Available with ROX in master mix and ROX as a separate vial |
Product Resources
Customers who bought these products also bought
- Cat No./ID:990399
Spin Column Adapter Rings (25)
Spin column adapter rings for use with spin columns that have screw caps; not required for all protocols$149.00Add To Cart - Cat No./ID:204484
QuantiFast Probe RT-PCR Plus Kit (400)
For 400 x 25 µl reactions: 1.3 ml 2x QuantiFast Mix 1 (x2), 1.3 ml 2x QuantiFast Mix 2 (x 2), 100 µl RT Mix, 210µl ROX Dye Solution, 210 µl High-ROX Dye Solution, 1.9ml RNase-Free Water$712.00Add To Cart - Cat No./ID:965662
QIAamp Virus BioRobot 9604 Kit (12)
For 12 x 96 nucleic acid preps: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), carrier RNA, caps$5,244.00Add To Cart - Cat No./ID:290013
mericon Salmonella spp Kit (24)
For 24 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution$209.00Add To Cart - Cat No./ID:211822
Certal CHO Detection Kit (100)
For 100 x 25 µl reactions: 1.25 ml Certal Residual DNA PCR Master Mix, Certal Internal Control DNA and Assay, CHO DNA Positive control and CHO-Assay, 100 µl Uracil-N-Glycosylase, 210 µl High-ROX Dye Solution, 210 µl ROX Dye Solution, Nucleic Acid Dilution Buffer, TE Buffer$1,719.00Add To Cart - Cat No./ID:1017647
Carrier RNA(poly A) 12x1350µg-15-25°C,KG
Carrier RNA(poly A) 12x1350µg-15-25°C,KG$234.00Add To Cart - Cat No./ID:208354
QuantiNova Probe RT-PCR Kit (500)
For 500 x 20 µl reactions: 3 x 1.7 ml QuantiNova Probe RT-PCR Master Mix, 100 µl QuantiNova Probe RT Mix, 100 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 1 ml ROX Reference Dye, 2 x 1.9 µl RNase-Free Water$663.00Add To Cart - Cat No./ID:204356
QuantiFast Probe PCR +ROX Vial Kit (2000)
For 2000 x 25 µl reactions: 25 ml 2x QuantiFast Probe PCR Master Mix (without ROX dye), 1.05 ml ROX Dye Solution, 20 ml RNase-Free Water$1,972.00Add To Cart - Cat No./ID:204456
QuantiFast Probe RT-PCR Kit (2000)
For 2000 x 25 µl reactions: 25 ml 2x QuantiFast Probe RT-PCR Master Mix (contains ROX dye), 0.5 ml QuantiFast RT Mix, 20 ml RNase-Free Water$2,840.00Add To Cart - Cat No./ID:57704
QIAamp MinElute Virus Spin Kit (50)
For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml)$295.00Add To Cart - Cat No./ID:204843
QuantiTect Multiplex RT-PCR NR Kit (200)
For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect Multiplex RT-PCR NoROX Master Mix (without ROX dye), 100 µl QuantiTect Multiplex RT Mix, 2 x 2 ml RNase-Free Water$1,006.00Add To Cart - Cat No./ID:981103
Strip Tubes and Caps, 0.1 ml (250)
250 strips of 4 tubes and caps for 1000 reactions of 10–50 µl$250.00Add To Cart - Cat No./ID:204001
Rotor-Gene SYBR Green PCR Demo Kit (80)
For 80 reactions: 2x Rotor-Gene SYBR Green PCR Master Mix, 10x QuantiTect Primer Assay for GPER, Standards, Unknown Samples, Buffer TE, RNase-Free Water$343.00Add To Cart - Cat No./ID:204745
QuantiTect Multiplex PCR NoROX Kit (1000)
For 1000 x 50 µl reactions: 25 ml 2x QuantiTect Multiplex PCR NoROX Master Mix (without ROX dye), 20 ml RNase-Free Water$2,983.00Add To Cart - Cat No./ID:208552
QuantiNova Multiplex RT-PCR Kit (100)
For 100 x 20 µl reactions: 0.5 ml 4x QuantiNova Multiplex RT-PCR Master Mix, 20 µl QuantiNova Multiplex RT-Mix, 20 µl QuantiNova IC RNA, 500 µl QuantiNova Yellow Template Dilution Buffer, 250 µl QN ROX Reference Dye, 1.9 ml RNase-Free Water$221.00Add To Cart - Cat No./ID:208652
QuantiNova Pathogen +IC Kit (100)
For 100 x 20 µl reactions: 1x 500 µl QuantiNova Pathogen Master Mix, 1 x 500 µl Yellow Template Dilution Buffer, 1 x 250 µl ROX Reference Dye, 1 x 1.9 µl RNase-Free Water, 1 x 1500 µl Nucleic Acid Dilution Buffer, 1 x 100 µl Internal Control RNA, 1 x 100 µl Internal Control DNA, 1 x 200 µl IC Probe Assay$302.00Add To Cart - Cat No./ID:204343
QuantiTect Probe PCR Kit (200)
For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect Probe PCR Master Mix, 2 x 2 ml RNase-Free Water$479.00Add To Cart - Cat No./ID:204954
QuantiFast Multiplex RT-PCR +R Kit (400)
For 400 x 25 µl reactions: 3 x 1.7 ml 2x QuantiFast Multiplex RT-PCR Master Mix (without ROX dye), 100 µl QuantiFast RT Mix, 210 µl ROX Dye Solution, 2 x 2 ml RNase-Free Water$1,037.00Add To Cart - Cat No./ID:204556
QuantiFast Probe RT-PCR +ROX Vial Kit (2000)
For 2000 x 25 µl reactions: 25 ml 2x QuantiFast Probe RT-PCR Master Mix (without ROX dye), 1.05 ml ROX Dye Solution, 0.5 ml QuantiFast RT Mix, 20 ml RNase-Free Water$2,781.00Add To Cart - Cat No./ID:15055-50
RNeasy PowerLyzer Tissue & Cells Kit (50)
Isolate total RNA from animal tissues or cells, optimized for use with bead-based homogenizers.$250.00Add To Cart
