Qiagen/QuantiNova Probe PCR Kit/For 100 x 20 µl reactions: 1 ml 2x QuantiNova Probe PCR Master Mix , 250 µl QN ROX Reference Dye, 500 µl QuantiNova Yellow Template Dilution Buffer, 1.9 ml Water/208252
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¥2220.00
美元价：
1332.00
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3392242852
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4000-520-616
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商品介绍
QuantiNova Probe PCR Kit
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For highly sensitive, specific, and ultrafast, probe-based real-time PCR
- Accurate detection of rare targets down to one copy
- Duplex capability for more results per reaction
- Reaction stability of up to 100 hours at 30°C for flexible workflows
- Visual indication of correct pipettingresulting infewer pipetting errors
- High specificity for better results
- Increased throughput due to ultrafast cycling
Avoid the deleterious effects of mispriming at lower temperatures with the QuantiNova Probe PCR Kit. Based on a novel, antibody-mediated, hot-start mechanism, the kit enhances the specificity and efficiency of probe-based real-time PCR to provide accurate, singleplex or duplex, cDNA or gDNA analysis on various real-time PCR cyclers. The added convenience of extreme stability for up to 100 hours at room temperature, without a need for any cooling agent, makes it ideal for handling of high-throughput samples and automated workflows. An in-built tracking system for visual identification of correct pipetting gives you absolute peace of mind while the extreme sensitivity for even low target amounts ensures reliable qPCR results every time.
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Cat No./ID:208252 QuantiNova Probe PCRKit (100) $111.00 For 100 x 20 µl reactions: 1 ml 2x QuantiNova Probe PCR Master Mix , 250 µl QN ROX Reference Dye, 500 µl QuantiNova Yellow Template Dilution Buffer, 1.9 ml Water |
Cat No./ID:208254 QuantiNova Probe PCRKit (500) $423.00 For 500 x 20 µl reactions: 3 x 1.7 ml 2x QuantiNova Probe PCR Master Mix, 1 ml QN ROX Reference Dye, 500 µl QuantiNova Yellow Template Dilution Buffer, 1.9 ml Water |
Cat No./ID:208256 QuantiNova Probe PCR Kit (2500) $1,638.00 For 2500 x 20 µl reactions: 15 x 1.7 ml 2x QuantiNova Probe PCR Master Mix, 5 x 1 ml QN ROX Reference Dye, 5 x 500 µl QuantiNova Yellow Template Dilution Buffer, 5 x 1.9 ml Water |
Cat No./ID:208257 QuantiNova Probe PCR Kit (7500) $3,516.00 For 7500 x 20 µl reactions |
The QuantiNova Probe PCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Built-in visual control for correct pipetting
The master mix supplied with the QuantiNova Probe PCR Kit contains an inert blue dye that does not interfere with the real-time PCR, but increases visibility in the tube or well. The QuantiNova Yellow Template Dilution Buffer contains an inert yellow dye. When the template nucleic acid, diluted with the QuantiNova Yellow Template Dilution Buffer, is added to the master mix, the color of the solution changes from blue to green (see figure Built-in pipetting control), providing a visual indication of correct pipetting and reaction setup.Reaction stability of up to 100 hours
The real-time PCR mix can be stored at 30°C for up to 100 hours without impairing the performance of subsequent reactions (Table 1). The outstanding stability, even after extended storage at room temperature without the use of any cooling agent, makes the QuantiNovaProbe PCR Kit ideal for high-throughput reaction setup and plate-stack handling (see table "Effect of storage on reaction stability").| Mean CT values | |||||
| QuantiNova Probe PCR Kit | Probe PCR kit from supplier L | ||||
| Amount in ng | 0 h | 100 h | 0 h | 100 h | |
| 10 | 24.37 | 24.49 | 24.35 | 27.49 | |
| 1 | 27.78 | 27.90 | 27.77 | 30.97 | |
| 0.1 | 31.16 | 30.98 | 31.37 | 34.48 | |
| No template control | Not detected | Not detected | Not detected | Not detected | |
Accurate and robust detection of a single DNA molecule
The high sensitivity of the QuantiNova Probe PCR Kit results in accurate and robust detection of even a single target copy (see figure Sensitive and robust detection of a single target copy). The special master mix supplied with these kits enables accurate quantification of 2 targets having widely differing abundance in a single tube. Thissaves time, money, and reduces the amount of sample material needed. Moreover,the duplex PCR data obtained is comparable with that obtained from a singleplex PCR (see figure Comparable results in duplex and singleplex PCR).Superior performance for difficult targets
The unique combination of QIAGEN"s proprietary and well-proven buffer technology along with the new QuantiNova DNA Polymerase, QuantiNova Antibody, and QuantiNova Guard, ensures real-time PCR success at the first attempt, without the need for costly and time-consuming optimization, even with challenging real-time PCR assays (see figure Superior results with challenging assays).Highly consistent results on all real-time cyclers
The QuantiNova Probe PCR Kit can be used on any real-time cycler. ROX is provided in a separate tube and can be added if using a cycler that uses ROX as a passive reference dye. The kit delivers highly consistent results among different cyclers in terms of sensitivity, reproducibility, and efficiency. The consistency of the results is maintained, despite varying fast-cycling capacities of the different cyclers that result in different cycling protocols (see figure Consistent resultswithvarious real-time PCR cyclers).Principle
The QuantiNova Probe PCR Kit delivers singleplex or duplex, cDNA or gDNA analysis with highest specificity because of a novel, antibody-mediated, hot-start mechanism (see figure Novel antibody mediated hot-start mechanism). At low temperatures, the QuantiNova DNA Polymerase is kept in an inactive state by the QuantiNova Antibody and a novel additive, QuantiNova Guard, that stabilizes the complex. This improves the stringency of the hot-start and prevents extension of nonspecifically annealed primers and primer–dimers. Within 2 minutes of raising the temperature to 95°C, the QuantiNova Antibody and QuantiNova Guardare denatured and the QuantiNova DNA Polymerase is activated enabling the PCR amplification.
Procedure
QuantiNova Probe PCR Kits contain ready-to-use master mixes that eliminate the need for optimization of reaction and cycling conditions. Simply add template gDNA or cDNA, primers, and probe to the master mix and follow the protocol in the handbook to get fast and reliable results on any real-time cycler. ROX passive reference dye is provided as a separate tube and the concentrations of the dye can be adjusted depending on the type of cycler used, enabling use of the QuantiNova Probe PCR kits on virtually any real-time cycler. Due to the optimized ROX concentrations, detection of even low copy numbers is achieved through automatic data analysis.
Applications
QuantiNova Probe PCR Kits can be used for probe-based gene expression analysis of cDNA targets and quantitative gDNA analysis on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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